Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"RNase H2"”.
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Structural Studies of the Mammalian RNase H2 Heterotrimeric Complex
RNase H enzymes are endonucleases that specifically catalyze the hydrolysis of ribonucleotides in RNA:DNA hybrids. They are classified into type 1 or type 2 based on amino acid sequence similarity, structure, and biochemical properties. Type 1 RNase Hs require a minimum of four ribonucleotides …
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DDI1/2 and RTF2-Dependent Regulation of RNASE H2 at the Replisome Ensures Genome Stability
… replisomes deficient for RTF2 concurrently lack RNase H2, a heterotrimeric enzyme responsible for removing RNA in the context of RNA-DNA heteroduplexes. Like RTF2, RNase H2 is necessary for normal replication speed. RTF2- deficient cells exhibit phenotypes consistent with loss of RNase H2 and an …
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Ribonuclease H2, RNA:DNA hybrids and innate immunity
… of one of the AGS nucleases, the Ribonuclease H2 (RNase H2) complex, in innate immunity, and to establish if nucleic acid substrates of this enzyme were able to induce type I interferon production in vitro. The AGS nucleases may function as components of the innate immune response to nucleic …
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Studies of the MYM-type zinc finger protein ZMYM3
RNase H2 is a trimeric ribonuclease that specifically degrades the RNA moiety of DNARNA hybrids. Mutations in RNase H2 result in Aicardi–Goutières syndrome (AGS), a rare inflammatory disorder notable for being a ‘mendelian mimic’ of congenital viral brain infection. Some AGS-associated mutations in …
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Functions of RTF2 in DNA Replication and Checkpoint Control
… that RTF2 recruits the heterotrimeric enzyme RNase H2 to the replication fork. Downstream of failed RNase H2 recruitment to the replisome, RTF2-deficient cells accumulate genomically embedded ribonucleotides. In DDI1/2-depleted cells, retention of RTF2 and RNase H2 at the replication fork …
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Functional Roles of Nucleases in DNA Metabolism and Genome Stability
… specifically Exonuclease 5 and Ribonuclease H2. First, I describe the biochemical and molecular characterization of the novel Exonuclease 5 family of enzymes from S. cerevisiae, S. pombe, and humans. The Exo5 family consists of bi-directional single-strand DNA specific exonucleases that all …
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Understanding of Intrinsic Sources of DNA Damage that Drive Human Disease: A Foray into the Study of DNA Interstrand Crosslinks and Genome-Embedded Ribonucleotides
… thesis concentrates on the study of the enzyme RNase H2 and its actions in ensuring genomic stability by removing genome-embedded ribonucleotides.</p>