Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"RNase E"”.
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… the RNA degradosome include the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the glycolytic enzyme enolase, and the phosphorolytic exoribonuclease PNPase. The degradosome has also been previously shown to associate with the RNA chaperone Hfq to form a small RNA guided machinery that …
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Endonuclease processing in the rif region of the Escherichia coli chromosome
… it is thought that endonuclease processing by RNase E and RNase III plays some role in translational regulation and/or degradation of these transcripts. The purpose of this study was to investigate the effect that RNase E and RNase III cleavage had on the stability of transcripts derived from …
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Biophysical characterization of the Escherichia coli RNA degradosome
… RNA degradosome consist of the endoribonuclease RNase E, the DEAD-box helicase RhlB, the glycolytic enzyme enolase, and the exoribonuclease PNPase. The main scaffold component of the RNA degradosome, RNase E, contains an amphipathic alpha helix that recruits the degradosome to the cytoplasmic …
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Synchrony of harmony and chaos in a dynamic, bacterial riboregulatory machine
… where the main component of the assembly is RNase E, a homotetrameric endoribonuclease. Each monomer of RNase E can be divided into two domains: the N-terminal domain which carries out the catalytic activity, and the C-terminal domain, which is intrinsically disordered but punctuated by …
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Characterization of 50S Ribosomal Subunit Assembly Inhibition in Erythromycin Treated <em>Escherichia coli</em> Cells.
… <em>in vivo</em> and <em>in vitro</em>.</p> <p>RNase E has been implicated in the normal degradation and turnover of rRNA. A RNase E-mutant accumulated substantially more precursor to the 50S subunit than did control cells at either 37°C or 27°C. This precursor particle was also found to bind …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… protect sRNAs from other ribonucleases such as RNase E and RNase III, and potentially lend their KH-S1 portal as an interaction platform to promote sRNA/RNA target pairing. A fraction of PNPase in the cell associates with the endoribonuclease RNase E to form the E. coli ‘RNA degradosome’, a …
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Understanding the physiological role of the small RNA RydC
… RydC and ArrS act through masking an RNase E cleavage site in the cfa mRNA 5' untranslated region (UTR), and both sRNAs post-transcriptionally activate cfa. In contrast, CpxQ binds to a different site in the cfa mRNA 5' UTR and represses cfa expression in a mechanism that is dependent …
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An Investigation of Bacterial Ribonucleases as an Antibiotic Target
… is processed by bacterial ribonucleases (RNases). RNases are also involved in the degradation and turnover of this RNA during times of stress, such as the presence of an antibiotic. This makes ribonucleases a potential target for novel antibiotics.</p><p>It was shown that <em>Escherichia …
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Regulation of glucosamine-6-phosphate synthase synthesis by a hierarchical acting cascade composed of two small regulatory RNAs in <i>Escherichia coli</i>.
… Kotranskript innerhalb des glmU-Stopp-Kodons von RNase E geschnitten. Hierdurch entsteht ein monocistronisches glmS-Transkript. Die Menge dieses glmS-Transkripts wird in Antwort auf eine intrazelluläre Limitierung von GlcN6P durch einen Rückkopplungsmechanismus reguliert. Für diese Regulation sind …
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Regulation der Synthese der Glukosamin-6-Phosphat Synthase GlmS in Escherichia coli durch das neuartige Protein YhbJ
… Regulation identifiziert, sowie ein Einfluß von Rnase E und Hfq beschrieben werden. Diese Regulation stellt einen der ersten beschriebenen Fälle dar, in denen eine kleine RNA die Transkription eines Gens innerhalb eines Operons positiv beeinflusst. Des Weiteren konnte die intrazelluläre …
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Regulation of the Mannose PTS Operon by the small RNA SgrS
… Regulation of manX did not require the RNase E degradosome complex, suggesting that the primary mechanism of regulation is translational. An Escherichia coli ptsG mutant strain that is manXYZ(+) experiences stress when exposed to the glucose analogs α- methyl glucoside or 2-deoxyglucose. …
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Post-Transcriptional Regulation of Virulence Genes by GlmY and GlmZ in Enterohemorrhagic E. Coli
… from the rest that requires the endoribonuclease RNase E. Overexpression of either sRNA results in the downregulation of the latter fragment of both the LEE4 and LEE5 operons. In the case of LEE4, this is through direct binding of GlmZ to a region within the LEE4 mRNA. We also investigated the …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… expression of hilD expression via the 3’UTR. RNase E and ProQ also affect the hilD expression via the 3’ UTR. Deletion of the 3’ UTR increases the hilD expression, causing overactivation of the SPI1. I suggest that the hilD 3’ UTR functions as destabilizing element of the hilD mRNA. Although I …
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… target region(s) likely lead to occlusion of RNase E binding. This mechanism was translated for expression of a diaphorase enzyme, which has relevance to synthetic biology and metabolic engineering in in vitro systems. Results were successful, showing a greater than 75% increase in diaphorase …