Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 165 for “"RNase"”.
-
Foamy Virus RNase H - Aktivität, Struktur und Funktion
… beinhaltet die Protease-, die Polymerase- und RNase H-Domäne. Letztere ist für den Abbau der RNA im entstehenden RNA/DNA Hybrid verantwortlich. Während die FV PR-RT als Monomer vorliegt, besteht die HIV-1 RT aus einem p66/p51-Heterodimer. Erstaunlicherweise ist die isolierte HIV-1 RNase H im …
-
Structural Studies of the Mammalian RNase H2 Heterotrimeric Complex
RNase H enzymes are endonucleases that specifically catalyze the hydrolysis of ribonucleotides in RNA:DNA hybrids. They are classified into type 1 or type 2 based on amino acid sequence similarity, structure, and biochemical properties. Type 1 RNase Hs require a minimum of four ribonucleotides …
-
Zusammensetzung eukaryotischer RNase P aus pflanzlichen Zellkernen und Plastiden
Ribonuklease P (RNaseP) ist eine essentielle Endonuklease, welche die 5'-Flanke von pre-tRNAs entfernt. In nahezu allen bisher untersuchten Organismen und Organellen besteht das Holoenzym aus einer RNA-Untereinheit und einer Protein-Komponente. Nur die Zusammensetzung des Enzyms in den …
-
Substratbindung und Katalyse in RNase P RNA vom cyanobakteriellen Typ
Ribonuklease P (RNase P) ist eine essentielle Endonuklease, welche die 5'-Flanke von pre-tRNAs entfernt. Die RNase P RNA des Cyanobakteriums Prochlorococcus marinus ist in vitro katalytisch aktiv und bevorzugt in heterologen Prozessierungssystemen Substrate mit vollständigem 3’-CCA-Ende. Diese …
-
Human RNase MRP complexes: The Rpp25-Rpp20 dimer makes the difference
Contains fulltext : 30946_humarnmrc.pdf (Publisher’s version ) (Open Access)
-
Restriction of Retrotransposition and Retroviral Infection by RNase L and APOBEC3G
The OAS/RNase L system is one of the major restriction pathways activated during the type I interferon response to viral infections. In this thesis I primarily explored its non-canonical functions beyond blocking viruses through degrading viral and cellular single-stranded RNA. Using …
-
Uncovering pathways necessary for the survival of RNase H-deficient Escherichia coli
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01
-
C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated …
-
DDI1/2 and RTF2-Dependent Regulation of RNASE H2 at the Replisome Ensures Genome Stability
… replisomes deficient for RTF2 concurrently lack RNase H2, a heterotrimeric enzyme responsible for removing RNA in the context of RNA-DNA heteroduplexes. Like RTF2, RNase H2 is necessary for normal replication speed. RTF2- deficient cells exhibit phenotypes consistent with loss of RNase H2 and an …
-
Discovery and biochemical characterization of Casl3b, a Type VI-B CRISPR-Associated RNA-Guided RNase
Targeted methods to perturb nucleic acid sequences or epigenetic modifications enable better understanding of nucleic acid function or dysfunction in disease. Particular CRISPR-associated (Cas) proteins are used for targeted DNA editing because they are easy to use, precise, and specific. …
-
Molekularbiologische Gewinnung von RNase 40-124 Fragmenten zur Synthese von einheitlichen Glycoproteinen durch native chemische Ligation
… 1-124 entwickelt werden. Zur Synthese der RNase durch NCL wurde das Cys 40 als Ligationsstelle gewählt. Das Fragment 40-124 B mit N-terminalem Cys sollte mit dem Thioester Met-RNase 1-39 A bzw. glycosylierten Varianten über sequentielle Ligationsschritte zur RNase ligiert werden. Zur …
-
Kinetic Characterization of the Coupled Folding and Binding Mechanism of Bacterial RNase P Protein: an Intrinsically Unstructured Protein
… systems. The protein component of bacterial RNase P holoenzyme from Bacillus subtilis (P protein) was used as a model system to elucidate the general folding/unfolding of an intrinsically unstructured protein (IUP) both in the absence and presence of ligands.</p><p>P protein was previously …
-
Σχεδιασμός και Σύνθεση Νέων Ετεροκυκλικών Χηλικών Συμπλεκτικών αναλόγων με αντι-HBV δράση μέσω αναστολής της RNase H του ϊού
… της HBV πολυμεράσης, αυτής της ριβονουκλεάσης Η (RNase H). Σε προηγούμενες έρευνες έχουν αναφερθεί αναστολείς από διάφορες χημικές κατηγορίες, όπως α-υδροξυτροπολόνες, Ν-υδροξυϊμίδια καθώς και κάποια φυσικά προϊόντα. Η παρούσα έρευνα επικεντρώνεται στις Ν-υδροξυπυριδινοδιόνες με πολλούς …
-
Endonuclease processing in the rif region of the Escherichia coli chromosome
… it is thought that endonuclease processing by RNase E and RNase III plays some role in translational regulation and/or degradation of these transcripts. The purpose of this study was to investigate the effect that RNase E and RNase III cleavage had on the stability of transcripts derived from …
-
Characterization of the mechanism of action of new HIV-1 reverse transcriptase-associated ribonuclease H inhibitors
… DNA dependent (DDDP) manner, and Ribonuclease H (RNase H) . The RNase H activity catalyzes highly specific hydrolytic events on the RNA strand of the RNA/DNA replication intermediate, critical to the synthesis of integration-competent double-stranded proviral DNA. Because of its essential role, …
-
Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
Ribonuclease III (RNase III) is a double-stranded-RNA-specific nuclease of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the …
-
Exploring and Exploiting Ribonuclease 1: from Protein Biochemistry to Protein Engineering
Ribonuclease (RNase) 1 is a human protein with a remarkable ability to indiscriminately hydrolyze RNA. RNase 1 and its bovine homologue RNase A exhibit ubiquitous expression across tissues, a catalytic efficiency within the diffusion-limited regime, and minimal substrate sequence requirements. …
-
Studies of the MYM-type zinc finger protein ZMYM3
RNase H2 is a trimeric ribonuclease that specifically degrades the RNA moiety of DNARNA hybrids. Mutations in RNase H2 result in Aicardi–Goutières syndrome (AGS), a rare inflammatory disorder notable for being a ‘mendelian mimic’ of congenital viral brain infection. Some AGS-associated mutations in …
Page 1 of 9