Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 5535 for “"RNa"”.
-
A GPU accelerated RNA-RNA interaction program
RNA-RNA interaction (RRI) is important in processes like gene regulation, and is known to play roles in diseases including cancer and Alzheimer's. Large RRI computations run for days, weeks or even months, because the algorithms have time and space complexity of, respectively, O(N3M3) and O(N2M2), …
-
Modification of Transfer Rna With T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of transfer RNAs. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of a tRNA resulting in a tRNA molecule one nucleotide longer with a 3' phosphate. Under appropriate conditions the reaction is quantitative and, if …
-
The structural basis of RNA-catalyzed RNA polymerization
… chemically identical to a single turnover of RNA-dependent RNA polymerization. Such an activity would have been requisite for the emergence of a self-replicase ribozyme, an enzyme that, according to the RNA World hypothesis, would be fundamental for the emergence of life. Demonstrating the …
-
RNA recognition: controlling RNA-protein complexes with small molecules
… I. Investigation of Small Molecule Binding to an RNA Hairpin Loop Containing a Dangling End PART II. Unraveling the Interaction of Pathogenic RNAs with the MBNL1 Protein and Complex Inhibition by Small Molecules PART I. RNA plays important and versatile roles in gene expression by both carrying …
-
Algorithm development for RNA structure prediction in RNA viruses
RNA structures play many different roles in the life cycles of RNA viruses, including in directing translational control, genome replication, subgenomic RNA synthesis, and encapsidation. However, predicting these structures can be challenging, especially when it comes to pseudoknots, long-range …
-
Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
An RNA-dependent RNA polymerase from ribosomes of maturing chicken erythrocytes was investigated. Concurrent with an increase in globin and RNA synthesis during phenylhydrazine induced anaemia, the total and specific activities of ribosome bound RNA-dependent RNA polymerase increased 40 and 9.4 …
-
Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
… were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA molecule (acceptor) to form a phosphodiester bond and the AMP portion is released. E. coli and D. melanogaster 5S RNA, yeast …
-
Computational Modeling of Rna-Small Molecule and Rna-Protein Interactions
<p>The past decade has witnessed an era of RNA biology; despite the considerable discoveries nowadays, challenges still remain when one aims to screen RNA-interacting small molecule or RNA-interacting protein. These challenges imply an immediate need for cost-efficient while predictive …
-
RNA CoMPASS: RNA Comprehensive Multi-Processor Analysis System for Sequencing
… pipeline for processing next-generation RNA sequencing (RNA-seq) data. RNA-seq experiments generate hundreds of millions of short reads for each DNA/RNA sample. There are many existing bioinformatics tools developed for the analysis and visualization of this data, but very large studies …
-
[RNA polymerase ribozymes]
The RNA World is a hypothetical ancient evolutionary era during which RNA was both genome and catalyst. During that time, RNA was the only kind of enzyme yet in existence, and one of its chief duties was the replication of RNA. This scenario presupposes that among all possible RNA sequences, there …
-
RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
The poliovirus (PV) RNA-dependent RNA polymerase (RdRP) is a small, single-subunit, enzyme that is responsible for the replication of the viral genome. PV genome replication is much more involved than just repetitively incorporating a single nucleotide into an oligonucleotide primer. The polymerase …
-
Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
… and encodes thousands of long noncoding RNAs (lncRNAs). Defining the mechanism of action of lncRNAs has been a critical challenge and priority for understanding their biological functions. An important example of this is the lncRNA NORAD, which is a highly conserved lncRNA that is …
-
Sequence and structure requirements of Y RNA-derived small RNA biogenesis
Numerous small non-coding RNAs have been identi�ed in mammalian cells, including microRNAs and piwi-interacting RNAs. The patterns of gene expression within cells can be altered in response to cellular stress. To examine the e�ects of cellular stress on all small RNA types, a number of human cell …
-
Targeting RNA With Small Molecules: SL3 of HIV -1 Psi-Rna
SL3 RNA of HIV-1 is the principal packaging determinant through its interaction with viral nucleocapsid protein, NCp7. Since SL3 is not a target of current anti-HIV therapeutics, the discovery of small molecules that target SL3 would not only expand the understanding of RNA recognition by small …
-
The Role of Rna and Rna Polymerases in The Aging Process
Radioactive uracil incorporated into the total RNA of old Rhizoctonia solani cells was twofold less than that in young cells, but there was no change in RNA label following a chase with unlabeled uracil. As the concentration of labeled uracil in the medium increased, the amount of uracil label in …
-
Ice as a medium for RNA-catalysed RNA synthesis and evolution
… self-replication and evolution. According to the RNA World hypothesis, this could have been an RNA polymerase ribozyme capable of generating copies of itself from simple nucleotide precursors. In vitro evolution experiments have provided modern examples of such ribozymes, such as the R18 RNA …
-
Development of site-specific RNA labeling strategies to probe alternative RNA splicing
… was to develop a method for the labeling of RNA at specific locations using non-natural base pairs. This thesis details the efforts made towards this objective through the development of a modular synthetic platform for an expanded genetic alphabet and its evaluation using transcriptional …
-
Studies of Plant Viroid RNA and Other RNA Species of Unusual Function
… dissertation concerns studies of plant viroid RNA and other RNA species of unusual function. By "RNA species of unusual function" we mean RNA species which do not function as transfer, ribosomal or messenger RNA. In the studies to be reported here we have laid the experimental and theoretical …
Page 1 of 277