Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"RNMT"”.
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
… group to the first transcribed nucleotide and RNMT methylates the guanosine cap at the N7-position.<br/>RNMT consists of a catalytic domain and an N-terminal domain that is absent in<br/>lower eukaryotes. Experiments presented in this thesis revealed that the N-terminus mediates RNMT …
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Investigating the cellular mechanisms that determine RNMT dependency in breast cancer cells
… In humans, RNA guanine-7 methyltransferase (RNMT) methylates the guanosine cap. RNMT expression is essential for cell viability and efficient gene expression.<br/><br/>This thesis demonstrates that a subset of breast cancer cells exhibit an enhanced dependence on RNMT for survival, in …
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Identification and functional characterisation of RAM, a novel and essential component of RNA guanine-7 methylation
… by the RNA guanine-7 methyltransferase, RNMT.<br/>RNA guanine-7 methylation is an essential process for cell viability and its up-regulation has been associated with cell transformation. However, the mechanistic details of RNMT function in mammalian cells remain elusive. In order to gain …
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The regulation and function of mRNA cap methylation in pluripotency and differentiation
… enzymes including the N7 RNA methyltransferase (RNMT), which along with its recently discovered activating subunit RAM, methylates the cap. RAM, which contains an RNA binding domain, is required to promote RNMT activity both in vitro and in vivo. Although the biochemical function of RAM has been …
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Investigating the role and regulation of mRNA capping in pluripotency and differentiation
… guanosine cap is methylated at N7 position by RNMT, which is in complex with its activating subunit RAM. Additionally, the first and second transcribed nucleotides can be methylated at ribose O2 position by CMTR1 and CMTR2 respectively. The mRNA cap protects transcripts from degradation and …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
… determination. The Cowling lab discovered that RNMT and RAM, which form the cap methyltransferase complex, are expressed at different levels in mouse primary tissues, and RAM is particularly highly expressed in mESCs. RAM is essential for pluripotency maintenance by controlling the expression of …
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Development and Application of Mass Spectrometry Based Methodologies to Study Ubiquitin-Like Modifiers
… substrate RNA guanine-7 methyltransferase (RNMT) revealed widespread SUMOylation at 16 different lysines by UBC9 despite lacking a consensus motif.<br/><br/>Finally, a quantitative proteomics workflow is presented that enriches whole proteome datasets by combining peptide identifications …