Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"RNF43"”.
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Development of a new screening system for the identification of RNF43-related genes and characterisation of other PA-RING family members.
The E3 ubiquitin ligase RNF43 (RING finger protein 43) is an important negative modulator of the WNT signalling pathway that acts at the plasma membrane by targeting Frizzled and its co-receptor LRP for degradation. In the small intestine, this prevents uncontrolled expansion of the stem cell …
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Application and Development of Advanced Genetic Tools to Study Adult Stem Cells
… these processes remain poorly understood. Rnf43 and its paralogue Znrf3 (RZ) act as tumour suppressors in the intestine, but their role in the gastric epithelium has not been previously investigated. Using a novel unpublished stomach specific CreERT2 expressing mouse line I found that …
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Genome-wide CRISPR Screens to Uncover Novel Therapeutic Targets in Wnt-β-catenin Driven Gastrointestinal Cancers
… Genome-wide CRISPR screens were completed in RNF43 mutant pancreatic ductal adenocarcinoma (PDAC) cells lines, which rely on Wnt-β- catenin autocrine signaling for their proliferation. These screens revealed a single Frizzled receptor gene out of ten paralogs, FZD5, to be essential for the …
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Establishment of new human and mouse liver cancer models and their use to uncover the role of RNF43 and ZNRF3 in liver homeostasis and repair
… technology. Interestingly, after mutating TP53, RNF43 and ZNRF3 either alone or in combination, human organoids undergo genetic alterations and phenotypic changes that partially resemble the ones observed in tumoroids. This data suggests that this system could be used as a screening platform to …
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Epigenetic Modulation In Braf Mutated Metastatic Colorectal Cancer
… regulation of Wnt/β-catenin signaling like RNF43 and Axin2 were significantly underexpressed in BRAF V600E mutant tumors when compared to their wild-type counterparts. These genes were hypermethylated in the xenograft models, which could be reversed with a demethylating agent.</p> …