Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 162 for “"RNAseq"”.
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L'analisi RNAseq della canna comune rivela dinamiche del trascrittoma fogliare se sottoposto ad un lungo periodo di stress salino
Le bioenergie possono fornire un contributo fondamentale nel soddisfare la crescente richiesta di produzione di energia sostenibile. La possibilità di assegnare terreni marginali alla coltivazione di piante da bioenergia rappresenta la principale strategia utile a superare il conflitto tra uso del …
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Genome Mining for Actinomycete Biosynthetic Gene Clusters
… of DSM4137 was analysed using total RNASeq and the results were used to inform analysis of HPLC/MS data of extracts under the same conditions. There was shown to be good correlation between the RNASeq results and the pattern of metabolites produced, suggesting that RNASeq may be a …
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Alternative isoform regulation in myotonic dystrophy
… developed a method for studying transcriptomes, RNAseq, which provides a high resolution, digital inventory of gene and isoform expression. By applying RNAseq to human tissues and cell lines, we discovered that essentially 92-94% of all human genes are alternatively spliced, 86% of them with a …
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Gene expression profiling approach towards enhancement of malaria vaccine development
… experiments using RNA sequencing technology (RNASeq) applied to samples collected during clinical trials performed at the CAUCASECO research center in Cali, Colombia. In each case, peripheral blood samples from volunteers and patients enrolled in studies addressing different aspects of …
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Within the skin: Grape berries during the mature stages of ripening
… whole genome oligonucleotide microarray and RNAseq technologies, proteins by nano-liquid chromatography-mass spectroscopy, and metabolites by gas chromatography-mass spectroscopy and liquid chromatography-mass spectroscopy. Principal components analysis of each of five Omic technologies …
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GENE REGULATION CONTROLLED BY THE CRBS/R TWO-COMPONENT SYSTEM
… genes under the control of the CrbS/R TCS. RNAseq analysis showed us that <em>acsA </em>and <em>actP, </em>which encodes an acetate permease, which is responsible for bringing acetate across the cell membrane,<em> </em>are upregulated by the TCS. Furthermore, in <em>P. aeruginosa </em>and …
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Small RNA studies in Drosophila melanogaster, Stylophora pistillata and Symbiodinium sp.
… the small RNAome of both organisms via small RNAseq. As the presence of a thick cell wall in Symbiodinium sp. poses a unique challenge to RNA extraction, and compounded by the dearth of literature regarding RNA extraction from the dinoflagellate, we optimised a procedure that consistently …
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SCI-Induced Chronic Pain is Driven by a Central to Peripheral IL-1 Mediated Signaling Pathway
… and hairy skin for 7 days following SCI. The RNAseq data shows that the expression of IL-1 family of cytokines, particularly IL-1β, was highly enriched at 1 day post injury in the SC and DRG at the level of injury but not below. Expression of the receptor IL-1R1 was greatly enriched in SC and …
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Protective Role of Mesenchymal MyD88 Signaling Under Homeostasis and Initiation of Inflammation in the Colon
… and cellular responses were analyzed by using RNAseq, qRT-PCR, ELISA, western blot, cytokine/chemokine multiplex array, flow cytometry, and confocal microscopy. In our first aim, I found that deletion of MyD88 within mesenchymal stromal cells in vivo resulted in inflammatory changes and …
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Molecular mechanisms of neuronal homoeostasis in vivo
… Kir2.1 and control neurones. Analysis of the RNAseq data revealed molecular candidates involved in homeostatic plasticity. In summary, I show that Kir2.1 over expression causes change in excitability and subsequent homeostatic plasticity in vivo. The mechanism of adaptation differs between …
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Partial GSK3 knockdown improves muscle performance in mdx mice: investigating the cellular mechanisms
… also being measured in the soleus as well. RNASeq analysis was conducted in gastrocnemius muscles obtained from mdx and mdx/GSK3KD mice. We saw an increase in glycogen content in both the EDL and soleus, an increase in the proportion of oxidative fibre types, along with a reduction in …
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Genomic Instability and Gene Dosage Obscures Clues to Virulence Mechanisms of F. tularensis species
… sequencing technologies. Additionally, RNASeq analysis was performed to identify differentially expressed genes between the different strains. This comprehensive genomic analysis revealed the critical role of transposable elements in inducing genomic instability resulting in large …
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Investigation of endogenous and adoptively transferred T cell function in a genetic mouse model of lung adenocarcinoma
… lungs of tumor bearing mice using single cell RNAseq (scRNAseq).
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The Neurogenomic State Underlying Selfish and Altruistic Aggression in Honey Bees (Apis mellifera)
… with selfish aggression of queens. Using RNAseq I compared the neurotranscriptome of at-rest and aggressive workers and queens. Classifying the differentially expressed genes (DEGs) of workers and queens as taxonomically-restricted or conserved as well as comparing these DEGs to that of …
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Zebrafish Posterior Lateral Line Organogenesis Regulation by Notch Signaling
… and the Hippo pathway. Transplantation and RNASeq analyses revealed that Notch signaling induces cell adhesion and tight junction proteins that interact with cytoskeleton causing cells to self‐organize into fewer larger organs rather than several smaller ones. Thus, Notch plays an essential …
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Computational Approaches to Understand Chemoresistance & Tumor Evolution Using Longitudinal Clinical Data and Lineage Tracing
… tumors pre and post-chemotherapy (N=131), using RNASeq and whole-exome sequencing, we interrogated switching of ART-types, changes in transcriptomic phenotypes, and genomic evolution. Androgen response subtype was the most stable, not impacted by chemotherapy, while high proliferating subtypes …
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Functional and Transcriptional Heterogeneity of the Human Haematopoietic Stem Cell Pool at Steady-State and Under Inflammation
… cell functional assays in vitro, RNA-sequencing (RNAseq) and in vivo assays to i) study lineage commitment heterogeneity within the HSC compartment of cord blood (CB) and foetal liver (FL) ii) to explore the role of inflammatory signals in HSC differentiation, using an in vitro model of human …
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Functions of The Trna Splicing Endonuclease and Other Adventures In Rna Processing
… genetic screens were used to complement our RNAseq approach to finding the other essential function of TSEN. A spontaneous suppressor screen identified mutations in Dbr1 as suppressors of only the other essential function a mutant sen2. Because mutations in Dbr1 could only complement a …
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Molecular and Cellular Investigations of Prader-Willi Syndrome
… and cycloid psychosis. Using RNA sequencing (RNAseq), we found a global decrease in mitochondrial transcript expression and a mitochondrial aggregate phenotype in PWS UPD +ASD neurons. In Chapter 3, we optimized a bioluminescent assay to visualize the circadian rhythms in PWS neurons. …
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Identification of gene expression changes in Drosophila models of mammalian prion diseases
… caused a specific toxicity. RNA sequencing (RNAseq) analyses demonstrated that, while there were some similarities in altered gene expression between the Drosophila model of ovine scrapie and those of gCJD and RML mouse-adapted scrapie, there were substantial differences at the same …
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