Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"RNAscope"”.
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The role of estradiol in astrocyte and neuron communication
… used an in situ hybridization technique known as RNAscope to examine estrogen receptor (ER) expression in the nucleus accumbens core (NAcC) and CA1 of the hippocampus (Hipp) of adult female and male mice. I then used calcium imaging techniques in hippocampal slices to monitor astrocyte calcium …
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Effect of age and pregnancy on the murine melanocortin system in the female reproductive system
… chromogenic RNA in situ hybridisation method (RNAscope®) enabled MC3, MC5 and MRAP2 localisation in the hypothalamus, in the preoptic (rostral and medial) areas and arcuate nucleus. Those areas are known to contain the gonadotropin-releasing hormone (GnRH) neuronal network in the female mouse …
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Cooperative Genomic Events Driving Mutant TP53-Driven DCIS Progression
… in the filled duct, DCIS-like phenotype. RNAscope revealed increased expression of <em>Tmem267 </em>in DCIS as compared to normal ducts, and a statistically significant increase in <em>Tmem267 </em>expression in mammary tumors. We nominate <em>TMEM267</em> as a cooperating event with …
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Behavioural and cellular basis of the vulnerability to develop compulsive heroin seeking habits
… of hotspot analysis, quantitative PCR, RNAscope and western-blot analyses, the data presented demonstrate that compulsive habits are associated with a differential pattern of cellular plasticity within corticostriatal networks, and are preceded by diverse cellular adaptations, especially …
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The Role of Glutamate in the Medial Prefrontal Cortex in the Acquistion and Expression of Conditioned Approach
… comparison to the respective control groups. An RNAscope was employed to assess whether the CS – a food-paired light stimulus –activated more neurons than the light stimulus not explicitly paired with food (neutral stimulus). The results revealed that the CS resulted in significantly higher …
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Hypothalamic control of energy rheostasis during metabolic challenges, pregnancy, and lactation
… observed was further characterized with RNAscope in situ hybridization, showing that female and male MC3R cells have different transcriptional profiles in DMH. Chapters 3 and 4 investigate neural adaptations supporting the increased energy intake of pregnancy and lactation, unique …
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Investigating upstream regulators of FOXA1 in breast cancer
… including NXF1, a nuclear RNA export factor. RNAscope analysis showed that NXF1 controls FOXA1 protein levels via regulation of FOXA1 mRNA nuclear export. Mechanistic experiments further revealed that NXF1 associates with nascent RNA co-transcriptionally on chromatin. Unbiased proteomics …
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Neurotransmitter Modulation of Alcohol-Induced GABAergic Signaling in the Central Amygdala
… reduced hypothalamic OXT neuron numbers, while RNAscope analyses demonstrated that chronic alcohol altered OXTR and CRF receptor expression in the CeA. Females expressed higher levels of OXTR and CRF mRNA regardless of alcohol history, whereas males, especially dependent males, exhibited greater …
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ROLE OF OXYTOCIN AND OXYTOCINASE IN THE MATERNAL RECOGNITION OF PREGNANCY
… by either immunohistochemistry (IHC), the RNAscope technique or by liquid chromatography tandem mass spectrometry (LC-MS/MS). The presence of LNPEP was confirmed with IHC and LC-MS/MS.
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Neural Plasticity in the Amygdala Across the Development of an Incentive Cocaine Seeking Habit
… as a marker of cellular activity, I performed RNAscope on brain sections encompassing the PFC to explore the behavioural relevance of specific PFC-BLA projections. Taken together the results presented in this work shed light on the mechanisms that may cause the shift in control over behaviour …
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Progesterone Regulation of the GnRH Pulse Generator
… kisspeptin neurons throughout the cycle using RNAscope. This revealed that *Pgr* mRNA levels in kisspeptin neurons remain unchanged across the cycle. Building on these findings, I next assessed the impact of circulating P4 on the activity of the GnRH pulse generator using ARN kisspeptin neuron …
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Interrogation of the neural mechanisms by which male and female rats assess aversive and mixed valence stimuli in learning and performing a task
… and devalued tasks. We used a new technology, RNAScope, to identify a population of dopamine receptor-expressing neuronal subtypes that are activated in the process of balancing reward and aversion during CTA. We found that the central nucleus of the amygdala (CeA) responds to hedonic and …
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Ontogeny and biological function of epithelial cells in the chicken yolk sac and small intestine
… during the embryonic and early posthatch stages. RNAscope in situ hybridization was used to identify the distribution of cells expressing PepT1 mRNA in both the chicken yolk sac and small intestine. PepT1 mRNA was found to be expressed by epithelial cells in both the yolk sac and small intestine. …
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The role of puberty in the development of the adolescent rodent medial prefrontal cortex and behavior
… in pre- and recently post-pubertal females using RNAScope, and note that Esr2 is significantly decreased in the post-pubertal subjects. A decrease in this receptor could have implications for adolescent behaviors that are influenced by estrogens, such as fear extinction or cognitive behaviors. …