Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 39 for “"RNAi screen"”.
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Large-Scale RNAi Screen to Identify Genes Involved in Axon Guidance in Caenorhabditis elegans
… beschriebene Methode der RNA Interferenz (RNAi) im Wurm zur Identifikation von neuen Genen der axonalen Wegfindung. Allerdings ist das Nervensystem von C. elegans resistent gegen systemische RNAi und Transport von doppelsträngigen RNA Molekülen in benachbarte nicht-neuronale Zellen …
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A Genome-Wide RNAi Screen Identifies CDC-42 and GDI-1 as In Vivo Regulators of Invadopodia
… and AC invasion. Using a sensitized genome-wide RNAi screen, classical genetics, and timelapse imaging of invadopodia at the AC-BM interface, Chapter 3 presents two in vivo invadopodia regulators that function by distinct mechanisms. This is the first in vivo evidence that the RhoGTPase CDC-42 …
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Epidermis and Re-epithelialization in <i>Schmidtea mediterranea</i>
… cell behaviors. In the pilot functional (RNAi) screen an array of transcription factors with a tissue maintenance phenotypes were identified. Our work established tools for subsequent functional studies of other epidermal expressed genes and paved the way to dissect the mechanisms of the …
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LDB-1 AND SWI/SNF CHROMATIN REMODELING COMPLEX PARTICIPATE IN THE TRANSCRIPTIONAL ACTIVATION BY C. ELEGANS BLIMP1/PRDM1
… as a transcriptional activator, we performed a RNAi screen and identified three possible interacting partners of BLMP-1. Specifically, we found that F58A3.1/LDB-1, F25H8.2 and ZK1128.5/HAM-3 positively regulate bed-3 in a BLMP-1 dependent manner, and that they do not act upstream of BLMP-1. …
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Muscle-derived cytokines: novel regulators of skeletal myogenesis
… are mostly unexplored. We performed a functional RNAi screen in order to better understand the roles of these muscle-derived cytokines in myogenesis, which is described in chapter II of this thesis. After screening 134 cytokine genes, we were able to categorize the resulting 29 positive hits into …
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Abdominal-B Neurons Control Drosophila Virgin Female Receptivity
… in this behavior. Using a genome-wide neuronal RNAi screen, we identified a requirement for Abdominal-B (Abd-B), a homeobox transcription factor, in virgin female sexual receptivity. Silencing adult Abd-B neurons in the abdominal ganglion and reproductive tract decreased female receptivity. …
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Experiments concerning the mechanism of cytokinesis in Caenorhabditis elegans embryos
… microtubule asters. To this end, I performed an RNAi screen, which identified a role for LET-99 in cytokinesis: LET-99 appeared to be required for aster-positioned cytokinesis but not midzone-positioned cytokinesis. LET-99 localizes as a cortical band that overlaps with the cytokinesis furrow. …
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Post-genomic studies on the neuropeptide signalling system of Juvenile <i>Fasciola hepatica</i>
… forward into a larger-scale RNA interference (RNAi) screen which revealed a high level of transcript knockdown and a profound growth phenotype for one member of the Neuropeptide-F/Y-like (NPF/Y) family. Profound motility and growth phenotypes were seen as a result of knocking down neuropeptide …
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Cellular functions and enzymatic activity of the poly(ADP-ribose) polymerase protein family
… each PARP. First a family-wide localization and RNAi screen was performed to identify novel functions for PARP family members. From this work, we determined that the majority of PARPs are cytoplasmic and discovered new cytoplasmic functions for PARPs including actin cytoskeleton regulation and in …
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Examining the Regulation and Function of Cancer Testis Antigens
… in CTAs after a pan-genomic-loss-of-function RNAi screen identified several CTAs as chemo-sensitizers. Acrosin Binding Protein (ACRBP) was identified in this screen and further analysis confirmed ACRBP's function as a microtubule stabilizer that protects cancer cells from the mitotic defects …
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Separate Roles of FAN1 and Fanconi Anemia Proteins in DNA Interstrand Crosslink Repair and Human Disease
… yeast to humans. It was first identified in an RNAi screen for proteins necessary for ICL repair and also as a direct interacting partner of FANCI/FANCD2 (ID2) complex of the Fanconi anemia pathway. Using cells isolated from human KIN patients and Fan1-deficient mice, we showed that ICL repair …
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Regulation of Wingless secretion, distribution and signaling
… on the cell surface. Importantly, from a genetic RNAi screen, I identified two essential components in the control of Wls ubiquitiation, the E3 ligase Su(dx) and the deubiquitinating enzyme USP8. My work suggests that Wls ubiquitination is involved in the trafficking of Wls and the sorting of Wg …
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Mechanisms of Type IV Collagen Targeting to Developing Basement Membranes
… conditional knockdown, misexpression, and RNAi screening techniques to investigate how the sole C. elegans type IV collagen molecule is recruited to the BMs of growing gonadal and pharyngeal organs during larval development. In Chapter 1, I review BM structure and functions, focusing on …
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Identification of the molecular pathways that mediate cytokine induced immunopathology of the gut epithelium
… by this cytokine combination. A human kinome RNAi screen and a drug repurposing screen identified the Janus kinases (JAKs) JAK1 and JAK2 as the major kinases required for IFN-γ + TNF-α dependent synergistic cell death. While TNF receptor-1 (TNFR1) was also required, there was no direct …
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RNA localizations of proteins that interact with the chromosome passenger complex
… compared throughout mitosis to that of Xist. An RNAi screen was used to identify that MCAK, a known target of AURKB’s kinase activity, is another player in the dispersal of Xist. Our results also demonstrated that nuclear envelope breakdown precedes the release of ncRNAs into the cytoplasm, and …
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ERK1/2 signalling and protein ubiquitylation in the control of apoptosis
… than specific inhibition of USP30. Finally, an RNAi screen, targeting 94 DUBs, and 8 sentrin/SUMO-specific proteases (SENPs), in the human genome, was performed to identify DUBs that modulate cell death induced by MEK1/2 or mTOR inhibition. As such, inhibitors of identified ‘hit’ DUBs might be …
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The Role of RUVBL1/RUVBL2 and Their Potential as Therapeutic Targets in Non-Small Cell Lung Cancer
… we performed a viability-based RNA interference (RNAi) screen targeting nuclear receptors, their coregulators and chromatin remodelers. This screen identified RUVBL1 and RUVBL2 (collectively referred to as RUVBL1/2) as differentially required for the viability of NSCLC. We show that RUVBL1/2 …
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Investigating toxicity in Drosophila models of Huntington's Disease and Huntington's Disease-Like 2
… HD. I also present the results from an in vivo RNAi screen to search for novel suppressors of toxicity in our HD and HDL2 models. Analyzing top RNAi suppressors from both models indicates different pathogenic pathways are at play in these two polyglutamine diseases, but some mechanisms may be …
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Regulation of the Kcnq1ot1 Imprinting Domain in Mouse
… selection, loss-of-function RNA interference (RNAi) screen. Depletion of candidates Ezh1, Smarca5 and Smarcad1 resulted in loss of imprinted expression of Cdkn1c and Kcnq1 but not Osbpl5 and Slc22a18, suggesting epigenetic modifiers identified here function at genes proximal to the Kcnq1ot1 ICR …
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