Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 547 for “"RNAi"”.
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RNAi-related genes in Toxoplasma gondii.
RNA interference (RNAi) is an evolutionarily conserved mechanism through which double-stranded RNA (dsRNA) triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced …
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Regulatory Mechanism of the RNAi Pathway
RNA interference (RNAi) is post-transcriptional gene silencing initiated by Dicer, a RNase III that processes double-stranded RNA (dsRNA) precursors into small interfering RNA (siRNA). In Drosophila, Dicer2 and R2D2 coordinately recruit duplex siRNA to the effector RNA-induced silencing complex …
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Biological Confinement of Zebrafish Using RNAi
… populations does not occur. RNA interference (RNAi) is an endogenous mechanism used to regulate gene expression by destroying targeted mRNA molecules. Manipulation of this biological process has been successfully utilized to knockdown specific genes through the introduction of synthetic …
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Application de la technique RNAi au silençage d’un rapporteur
Le système d’interférence par ARN (RNAi) a été découvert récemment. A la base, il semblerait avoir constitué un mécanisme de défense naturel de l’organisme en réponse à l’expression anormale de gènes, notamment ceux résultants de l’envahissement viral ou de transposons. A l’heure actuelle, il est …
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Development and characterization of an inducible RNAi system in Toxoplasma gondii
… have constructed a tetracycline based inducible RNAi vector which can be used for regulating gene expression in T. gondii. Upon the addition of anhydrotetracycline, the production of double stranded RNA homologous to the target gene is induced. The inducible vector was characterized using the …
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RNAi Nanotechnology: A Platform for siRNA Screening and Cancer Gene Therapy
… of applications, including RNA interference (RNAi) and the regulation of mRNA expression. These important biological functions have been implemented in gene therapy and for screening malignant gene targets. In spite of their therapeutic potential, naturally occurring siRNAs are limited by poor …
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Rapid Functional Dissection of Genetic Networks Via RNAi In Mouse Embryos
<p>The development and maintenance of epithelial tissues is regulated by a complex array of signal cues from adjacent cells, the extracellular milieu and intracellular signaling cascades. In the mammalian epidermis, these cues instruct the specification and invagination of hair follicles as well as …
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A gene signature for Alzheimer’s disease using RNAi in C. elegans
Alzheimer’s disease (AD) is a complex multifactorial disorder that is responsible for the large majority of the 50 million cases of dementia worldwide. This disease is still incurable, a situation caused at least in part by the fact that its genetics are incompletely known. In our laboratory, we …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… in the advent of the first RNA interference (RNAi) application in humans. RNAi solicits the use of a double-stranded RNA substrate, aptly named short-interfering RNA (siRNA), which binds to and triggers the degradation of a targeted complementary mRNA strand within the catalytic site of the …
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The role of RNAi genes in Neurospora crassa post-transcriptional gene silencing
Meiotic silencing was first identified in the bread mold Neurospora crassa (by our group and others) and has since been discovered in worms, mice, and humans. Neurospora is an excellent model organism in that it is inexpensive, has a relatively small number of genes, and a rapid life cycle with two …
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Investigation of factors involved in RNAi-mediated heterochromatic silencing in Schizosaccharomyces pombe
… formation can be targeted by RNA interference (RNAi), a conserved regulatory mechanism mediated by small RNAs. RNAi-mediated heterochromatin assembly has been extensively studied in the fission yeast Schizosaccharomyces pombe, where RNAi is strictly required for the establishment and maintenance …
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Elucidating the role of effector caspases in immune development using lentiviral RNAi
… them is important. We used RNA interference (RNAi) to examine their roles in immune cells. RNAi has revolutionized the field of mammalian genetics by expediting the interrogation of gene function. Endogenous genes are targeted for silencing by the introduction of double stranded RNAs, known as …
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Endogenous RNAi pathways are required in neurons for dauer formation in Caenorhabditis elegans
… that endogenous RNA interference (endo-RNAi) pathways play a pivotal role early in the life cycle of worms in the detection of environmental conditions and mediating the appropriate developmental decisions. Our findings illustrate that functional endo-RNAi pathways contribute to the …
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The Role of RNAi in Mammalian Cells in Response to Sindbis virus infection
… was verified by northern blotting. A functional RNAi assay was developed to assess the activity and function of the RNAi system in cells subjected to cellular stress, type I interferon, infection and dsRNA, and northern blotting was used to verify the sequencing data. I have found that certain …
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RNAi em Colletotrichum lindemuthianum: caracterização das proteínas-chave e desafios para a aplicação
… Neste contexto, o RNA de interferência (RNAi) surge como uma alternativa promissora, permitindo o silenciamento de genes essenciais para a patogenicidade do fungo. Com isso, o presente estudo teve como objetivos: caracterizar os principais componentes da maquinaria de RNAi em C. …
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An in vivo RNAi therapy screen identifies novel mediators of leukemia-microenvironment interaction
… experience a relapse. We have adapted an in vivo RNAi screening approach to perform novel longitudinal studies both in mice and in cultured cells to identify genetic mediators of response to the TKI dasatinib in a transplantable syngeneic mouse model of BCR-ABLI+ BCP-ALL. The unique combination of …
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Large-Scale RNAi Screen to Identify Genes Involved in Axon Guidance in Caenorhabditis elegans
… beschriebene Methode der RNA Interferenz (RNAi) im Wurm zur Identifikation von neuen Genen der axonalen Wegfindung. Allerdings ist das Nervensystem von C. elegans resistent gegen systemische RNAi und Transport von doppelsträngigen RNA Molekülen in benachbarte nicht-neuronale Zellen …
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