Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"RNAP II"”.
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Transcription Coupled DNA Repair in Saccharomyces Cerevisiae: the Interplay of Facilitators and Repressors
… and facilitators interplay with RNA polymerase II (RNAP II) to carry out TCR in yeast Saccharomyces cerevisiae. By site-specific incorporation of the unnatural amino acid p-benzoyl-L-phenylalanine, we mapped interactions between Spt5 and RNAP II in S. cerevisiae. Through its KOW4-5 domains, Spt5 …
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Protein coding transcription and control of vsp gene expression in the protozoan parasite Giardia lamblia
… selective inhibitor of eukaryotic RNA Polymerase II (RNAP II), and is commonly used to study RNAP II transcription. Therefore, we measured the sensitivity of G. lamblia RNAP II transcription to α-amanitin and found that unlike most other eukaryotes, RNAP II transcription in Giardia is resistant to …
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Regulation of transcription in Plasmodium falciparum, the causative agent of severe malaria: initial characterisation of PfTBP and PfTFIIA
… the assembly and function of the RNA polymerase II (RNAP-II) transcription initiation complex composed of RNAP-II and the general transcription factors (GTFs). Bioinformatics analyses show that the RNAP-II GTFs in P. falciparum have greatly diverged from those studied in other eukaryotes, …
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A Structural Approach to Unveil the Role of BRCA1 in the Context of Transcription
… intricate manner in which BRCA1 interacts with RNAP II during mRNA production utilizing a unique microchip system developed in our lab. We were first able to prove the effectiveness of our tunable system using a breast cancer model of patient derived triple negative breast cancer (TNBC) cells. …
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Prevalence And Physiological Significance Of Gene Looping In Saccharomyces Cerevisiae
… gene looping. In this study we showed that the RNAP II subunit Rpb4 has a significant effect on termination of transcription. Gene looping is disrupted in the absence of Rpb4. Rpb4 shows a strong physical interaction with the Mediator subunit Srb5. Mediator subunit Srb5 crosslinked to the 5' and …
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An integrated CRISPR-Cas toolkit for engineering human cells
… regulatory strategies including RNA Polymerase II (RNAP II) promoters, RNAtriple- helix structures, introns, microRNAs and ribozymes, we demonstrate efficient modulation of endogenous promoters and the implementation of tunable synthetic circuits such as multistage cascades and RNA-dependent …
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General Transcription Factors Play Dual Roles In Initiation And Termination
… is unknown. The general transcription factors TFIIB and TFIIH have been found to interact genetically with Ssu72, a component of CPF 3' end processing complex. Accordingly, we found that TFIIB and TFIIH localize to the distal ends of genes in a transcription dependent manner. TFIIB localization …
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The functions of the RNA polymerase II CTD in transcription and RNA processing
RNA polymerase II (RNAP II), transcribing messenger RNAs (mRNAs), small nuclear RNAs (snRNAs), and non-coding RNAs (ncRNAs), is composed of 12 subunits. Rpb1, the largest subunit with catalytic polymerase activity, possesses a unique c-terminal domain (CTD) that consists of tandem heptad repeats …
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
RNA Polymerase II (Pol II) is the primary actor in the transcription of mRNA from genes. Pol II is a complex composed of twelve protein subunits. This study focused on the changes in the interactome of Rbp3 in S. cerevisiae when the Pol II subunit Rpb9 is removed. Rpb3 is one of the core subunits …