Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"RNAP"”.
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A PLATFORM FOR THE DISCOVERY AND CHARACTERIZATION OF PROTEINS THAT ASSOCIATE WITH PSEUDOMONAS AERUGINOSA RNA POLYMERASE
… discovery of novel drug targets. RNA polymerase (RNAP) is an essential enzyme controlling gene regulation, and its activity is modulated through a plethora of transcription factors or other proteins. The regulation of gene expression has been best studied in <em>E. coli</em>.</p> <p>The aim of …
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Cryo-EM Studies of Bacterial RNA Polymerase
<p>In bacteria, a single RNA polymerase (RNAP) performs all transcription. The overall structure of bacterial RNAP resembles a crab claw with pincers comprising the β' and β subunits and a large cleft where the active site sits. Structural information about this essential enzyme has mainly been …
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Structural Studies of Three Factors That Affect the Prokaryotic Transcription Cycle; Microcin J25, LAMBDA Q and T4 GP33
… cycle; Microcin J25, a peptidyl inhibitor of RNAP; Q antiterminator protein, a bacteriophage protein that modifies RNAP to readthrough termination signals in phage late genes; and Gp33, a T4 phage protein that is a co-activator of T4 phage late gene transcription. We determined that the …
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Mechanistic studies on transcription activation via DNA looping in a prokaryotic promoter-enhancer system
… with the alternative sigma factor sigma54 (RNAP-sigma54), and the transcription activator protein NtrC (nitrogen regulatory protein C), which binds to a remote enhancer region to the promoter. Enhancer-bound NtrC contacts the RNA polymerase at the promoter by means of DNA looping (closed …
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Mechanism of Transcription Arrest By The Nun Protein of Bacteriophage HK022
… et al, 2008). Escherichia coli RNA polymerase (RNAP) and its auxiliary transcription factors have long been studied as a model for understanding regulation of elongation, but biochemical insights into some of these mechanisms still must be elucidated. In this study I explored the mechanism of …
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A Structural Perspective on Bacterial Transcription
… process. In the first step, RNA polymerase (RNAP) contacts with the promoter DNA are established and then must be broken for the enzyme to transition into the elongation phase of RNA production; a process known as promoter escape. While single-molecule and biochemical observations report that …
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Engineering the interface between cellular chassis and synthetic biological systems
… chassis expressing both T7 RNA polymerases (RNAP) and orthogonal ribosomes that are unused by the chassis but are available for use by an engineered system. I implement a network in which the orthogonal ribosomal RNA and the gene encoding T7 RNAP are transcribed by T7 RNAP. In turn, the …
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Redefining the Coordination of Gene Expression Machineries in Bacillus subtilis
… as the ribosome binding site emerges from the RNAP, the ribosome can initiate translation, and both physically associate and kinetically coordinate with the RNAP over the course of transcription. This close proximity between the RNAP and ribosome allows the ribosome to modulate the fate of the …
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Structural and Functional Studies of Mycobacterial General Transcription Factors RbpA and CarD
… have used Escherichia coli (Eco) RNA polymerase (RNAP) as a model for studying prokaryotic transcription initiation. However, properties of Eco RNAP are not representative of RNAPs from other bacterial species. Transcription in mycobacteria is distinct from Eco by its formation of unstable …
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Characterization of the Omega Subunit of Borrelia Burgdorferi’s RNA Polymerase
… gene expression carried out by RNA polymerase (RNAP). The RNAP holoenzyme consists of two ɑ subunits, a β subunit, and a β’ subunit. Additional proteins have been implicated in the function of RNAP including RpoZ (i.e. omega subunit, ω). Investigations of RpoZ homologues from other bacteria have …
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Transcription Coupled DNA Repair in Saccharomyces Cerevisiae: the Interplay of Facilitators and Repressors
… facilitators interplay with RNA polymerase II (RNAP II) to carry out TCR in yeast Saccharomyces cerevisiae. By site-specific incorporation of the unnatural amino acid p-benzoyl-L-phenylalanine, we mapped interactions between Spt5 and RNAP II in S. cerevisiae. Through its KOW4-5 domains, Spt5 …
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New mechanisms in transcription regulation and chromosome organization in Caulobacter crescentus
… that interact with DNA or RNA polymerase (RNAP) or both, and can change the preference of RNAP for target promoters. Additionally, DNA is tightly compacted and organized inside cells. In bacteria, nucleoid-associated proteins (NAPs) play critical roles in shaping and compacting the …
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Characterization of Bacterial Transcriptional Regulatory Networks through Genome-Wide in vitro Run-Off Transcription RNA-Seq (ROSE)
… DNA, ribonucleotides, RNA polymerase (RNAP) core enzyme, and a specific sigma (σ) factor that recognizes the corresponding promoters that have to be analyzed. ROSE was carried out on Escherichia coli K-12 MG1655 genomic DNA using the E. coli RNAP holoenzyme, which includes σ70. This …
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Die Regulation der prokaryontischen Transkription auf molekularer Ebene
… fünf Untereinheiten bestehende RNA Polymerase (RNAP) katalysiert. Für die Bildung der Phosphodiesterbindung in der RNA sind die beta- und beta’-Untereinheiten verantwortlich. Die aminoterminalen Domänen der beiden alpha-Untereinheiten sind für den korrekten Aufbau zuständig, während die durch …
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A model for sigma factor competition in bacterial cells
… for binding to a limited pool of RNA polymerase (RNAP) core enzymes, providing a mechanism for cross talk between genes or gene classes via the sharing of expression machinery. To quantitatively analyze the contribution of sigma factor competition to global changes in gene expression, we develop a …
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The Modulation of Innate Immunity by Cellular and Viral Noncoding RNAs
… RNAs are RNA polymerase III transcripts (RNAP III) which when dysregulated trigger the innate immune system. In the second part of this thesis, we show that the RNA binding protein YBX1 prevents the activation of inflammation in the absence of exogenous immune stimuli. Notably, YBX1 …
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Structural Analysis of an RNA Polymerase Sigma Factor and Its Anti-sigma: sigma28/FLGM
… is a dissociable subunit of RNA polymerase (RNAP), and sequence-specifically recognizes promoters, but only after associating with RNAP to form the holoenzyme. In the holoenzyme, the major sigma domains (sigma2, sigma3, and sigma4) are spread across the surface, with the promoter binding …
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Protein coding transcription and control of vsp gene expression in the protozoan parasite Giardia lamblia
… inhibitor of eukaryotic RNA Polymerase II (RNAP II), and is commonly used to study RNAP II transcription. Therefore, we measured the sensitivity of G. lamblia RNAP II transcription to α-amanitin and found that unlike most other eukaryotes, RNAP II transcription in Giardia is resistant to 1 …
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Regulation of transcription in Plasmodium falciparum, the causative agent of severe malaria: initial characterisation of PfTBP and PfTFIIA
… assembly and function of the RNA polymerase II (RNAP-II) transcription initiation complex composed of RNAP-II and the general transcription factors (GTFs). Bioinformatics analyses show that the RNAP-II GTFs in P. falciparum have greatly diverged from those studied in other eukaryotes, suggesting …
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Studies into Transcriptional Pausing Regulators and Drug Resistance in Mycobacterium Tuberculosis
… We are therefore studying RNA polymerase (RNAP), the central enzyme of transcription, and associated transcription factors. We aimed to study pausing, an essential regulatory step occurring during elongation. Elongation is not constant but is instead intermittently interrupted by “pausing” …
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