Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"RNA-protein interactions"”.
-
Biochemical and Genetic Analyses of Rna-Protein Interactions
The coat protein from bacteriophage R17/MS2 functions to repress translation of replicase and functions to encapsidate the RNA genome of the phage. The coat protein accomplishes these functions by interacting with a 21 nucleotide stem-loop structure located within the translation initiation region …
-
Computational Modeling of Rna-Small Molecule and Rna-Protein Interactions
<p>The past decade has witnessed an era of RNA biology; despite the considerable discoveries nowadays, challenges still remain when one aims to screen RNA-interacting small molecule or RNA-interacting protein. These challenges imply an immediate need for cost-efficient while predictive …
-
"fine-tuning" of ribosomal structure and functions by pseudouridylation and rna-protein interactions
… structure and functions by pseudouridylation and RNA-protein interactions</p> <p>by</p> <p>JUN JIANG</p> <p>AUGUST 2012</p> <p>Advisor: Prof. John SantaLucia Jr.</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> <p>Ribosomal structure and functions appear to be …
-
The Development and Application of Methods to Study the Evolution of Specificity, Allostery, and RNA -Protein Interactions in Translation
… used to identify a putative class II cysteinyl-tRNA synthetase (CysRS) in several archaea that eluded previous annotation studies. This enzyme was found to be involved in the indirect pathway of cysteine aminoacylation that is shown to exist in the last universal common ancestral state. We have …
-
Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
RNA-protein interactions underscore a broad array of regulatory mechanisms throughout biology, and dysregulation of these interactions contributes to a broad range of human pathologies such as neurodegenerative disorders and many cancers. Recent studies have discovered thousands of RNA-binding …
-
Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
… aminoacylation, amino acid editing, and RNA-protein interactions.
-
Protein-Protein and Protein-Rna Interactions in the in Vitro Assembly of the R17 Bacteriophage
… R17 bacteriophage is a positive single stranded RNA virus that specifically infects male strains of E. coli. The role of RNA protein interactions in the in vitro assembly of the virus is examined in order to determine both what features of the RNA are important for its selective packaging by R17 …
-
The evolution, modifications and interactions of proteins and RNAs
Proteins and RNAs are two of the most versatile macromolecules that carry out almost all functions within living organisms. In this thesis I have explored evolutionary and regulatory aspects of proteins and RNAs by studying their structures, modifications and interactions. In the first chapter of …
-
Single molecule pull-down of viral RNA-protein complexes
Protein and RNA molecules interact with multiple protein partners to perform essential cellular processes such as post-transcriptional regulation of mRNA. Recently, a single molecule pull-down (SiMPull) assay was developed to isolate and study single protein complexes directly from cell lysates. …
-
1. X-ray crystallographic analyses of the gene V protein from Ff bacteriophage, a single-stranded DNA-binding protein, and its Y41 mutants 2. X-ray crystallographic analyses of several anticancer drugs and their interactions with DNA
In biological systems, DNA/RNA-protein interactions as well as drug-DNA interactions play important roles in genetic processes. A fundamental understanding of these processes requires a detailed structural knowledge of DNAs, proteins, drugs, as well as their complexes with each other. Gene V …
-
Establishing new approaches to unveil regulatory functions of tRNAs and their interactors
… study living systems. However, once the role of tRNAs was established in protein synthesis, the field moved on to other areas of the RNA world. With the mainstream application of systems-wide approaches, tRNAs have emerged as implicated in multiple regulatory networks beyond translation. These …
-
Multiple regulatory layers in the establishment of Rbfox2 splicing networks
Regulated alternative splicing is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define …
-
Relating structure and function: Discovery of novel inhibitors of myotonic dystrophy
… The structure of the expanded DNA and RNA repeats that cause myotonic dystrophy type 1 (DM1) present a unique opportunity to develop sequence-selective small molecule inhibitors that bind to the DNA or RNA and inhibit RNA synthesis or aberrant RNA-protein interactions directly as well …
-
Novel RNA Targets of the Spinal Muscular Atrophy Protein
Ribonucleoprotein complexes (RNPs) are involved in many essential cellular processes, of which the most prominent examples include the ribosome that functions in protein translation and the spliceosome which catalyzes pre-mRNA splicing. The biogenesis of RNPs often involves complex and elaborate …
-
Targeting HIV-1 RNAs with Medium Sized Branched Peptides Featuring Boron and Acridine-Branched Peptide Library Design, Synthesis, High-Throughput Screening and Validation
RNAs have gained significant attention in recent years because they can fold into well-defined secondary or tertiary structures. These three dimensional architectures provide interfaces for specific RNA-RNA or RNA-protein interactions that are essential for biological processes in a living system. …
-
M6A Reshapes the Folding and Recognition Landscape of RNAs
<p>Ribonucleic acid (RNA) is a versatile and dynamic biomolecule that serves as an indispensable component in the central dogma of molecular biology. The realization that RNA plays a wide variety of roles in gene expression and regulation has been accompanied by the discovery that virtually all …
-
Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… a trisegmented, single-stranded, negative-sense RNA genome, consisting of L, M and S segments. The virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA …
-
Inferring Transcriptional and Post-Transcriptional Network Structure by Exploiting Natural Sequence Variation
… has made it possible to use messenger RNA levels as quantitative traits in linkage studies. This has created new opportunities to study genetic variation at the level of gene regulatory networks rather than individual genes. This thesis consists of four parts, each of which outlines a …
Page 1 of 2