Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"RNA-dependent RNA polymerase"”.
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
The poliovirus (PV) RNA-dependent RNA polymerase (RdRP) is a small, single-subunit, enzyme that is responsible for the replication of the viral genome. PV genome replication is much more involved than just repetitively incorporating a single nucleotide into an oligonucleotide primer. The polymerase …
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Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
An RNA-dependent RNA polymerase from ribosomes of maturing chicken erythrocytes was investigated. Concurrent with an increase in globin and RNA synthesis during phenylhydrazine induced anaemia, the total and specific activities of ribosome bound RNA-dependent RNA polymerase increased 40 and 9.4 …
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Using Altered Fidelity Viruses to Study Viral Attenuation and Population Genetics During Vertebrate Host Infection
… diversity within the population is essential. RNA viruses contain an RNA dependent RNA polymerase (RdRp) that creates a population of closely related but slightly different variants following each replication cycle. These variants can interact with each other, allowing the viral population to …
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Genetic and Biochemical Properties of Arabidopsis RNA Polymerases IV and V
RNA Polymerases IV and V: Pol IV and Pol V) are plant-specific enzyme complexes with subunit homology to RNA Polymerase II: Pol II). The largest subunits in Pol IV and Pol V, NRPD1 and NRPE1 respectively, share a second largest subunit, NRPD2/NRPE2. The evolutionarily conserved Metal A and Metal B …
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Role of basic and hydrophobic residues in the poliovirus polymerase elongation complex and the structure of a coxsackievirus polymerase elongation complex
Picornaviruses encode for and require a viral RNA-dependent RNA polymerase (RdRP) for genome replication. This enzyme synthesizes negative-sense RNA from the infecting positive sense genome producing a replicative intermediate. The negative sense RNA then serves as a template for synthesis of …
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Adenosine triphosphate is an allosteric inhibitor of coxsackievirus B3 3Dpol
Picornaviruses pose a significant threat to human and animal health, but at present there are no drugs to prevent or treat picornaviral infections. However, intensive study of the picornaviral lifecycle has revealed several promising pharmacological targets, including the RNA-dependent RNA …
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Translating biomedical research data to knowledge through bioinformatics
… macrophages experienced mixed classic and alternative polarization/activation and whole spleen tissue experienced massive inflammatory response during visceral leishmaniasis. We proposed several mechanisms to understand the pathogenesis of L. donovani. Additionally, we investigated genomics …
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The Function and Mechanism of RNA Interference in Neurospora
RNA interference (RNAi) is a conserved gene silencing mechanism important for various biological processes, including developmental timing, genome defense, and heterochromatin formation. RNAi is triggered by double stranded RNA (dsRNA), which is processed by Dicer to siRNA. siRNA is loaded onto …
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Norovirus Translation and Replication
… major capsid protein VP1 interacts with viral RNA-dependent RNA polymerase (RdRp) in a genogroup-specific manner to enhance de novo initiation of RdRp, and to promote negative-strand RNA synthesis. To understand how VP1 regulates norovirus genome replication, chimeric MNVs with …
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Investigation of mosquito (diptera: culicidae)-borne viruses circulating in some selected areas of Lusaka district, Zambia.
Mosquito-borne viruses are RNA viruses cutting across different virus families and genera of emerging and re-emerging viruses that account for millions of disease incidences annually worldwide. Since the circulation of viruses in vectors heralds an outbreak of diseases, this present cross-sectional …
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Investigation of mosquito (diptera: culicidae)-borne viruses circulating in some selected areas of Lusaka district, Zambia.
Mosquito-borne viruses are RNA viruses cutting across different virus families and genera of emerging and re-emerging viruses that account for millions of disease incidences annually worldwide. Since the circulation of viruses in vectors heralds an outbreak of diseases, this present cross-sectional …
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GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae
… bushy stunt virus (TBSV) is a positive-sense RNA virus and its genome replication requires two viral proteins, p33 and RNA-dependent RNA polymerase p92. Previous studies showed that the 5 untranslated region (5UTR) of the genome is important, albeit not essential, for viral RNA replication in …
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Biochemical and Structural Characterization of Divergent Members of the Protein Kinase Family
… Glutamylation, and NiRAN-catalyzed RNA capping. A Legionella pneumophila effector SidJ adopts a kinase fold yet catalyzes calmodulin (CaM)-dependent glutamylation to inactivate the SidE ubiquitin ligases. SidJ becomes activated upon translocation into the host cell and uses cellular …
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Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… a trisegmented, single-stranded, negative-sense RNA genome, consisting of L, M and S segments. The virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA …
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Characterization of a Low-Fidelity Live-Attenuated Vaccine for Venezuelan Equine Encephalitis Virus
During RNA virus replication, there is the potential to incorporate mutations that affect virulence or pathogenesis. For live-attenuated vaccines, this has implications for stability, as replication may result in mutations that either restore the wild-type phenotype via reversion or compensate for …
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Biochemical mechanisms for selectivity governing RNA and DNA replication
Enzymes that catalyze DNA and RNA replication are biologically important and provide examples to understand enzyme specificity as the correct substrate and alternate substrates are well known. The DNA polymerase from bacteriophage T7 has been a model system for understanding high-fidelity DNA …
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Determinants of Core Shell Dependent Rotavirus Polymerase Activity
… of childhood mortality in many countries. The RV RNA-dependent RNA polymerase, VP1, synthesizes RNA during viral replication only in the presence of another RV protein, VP2, which comprises the innermost core shell layer of the virion. Though these VP1-VP2 interactions are essential for RV …
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The Mechanism of RNA Interference in Neurospora
In the canonical RNA interference (RNAi) pathway, small-interfering RNA (siRNA) duplexes generated by Dicer are incorporated into the RNA-induced-silencing complex (RISC), and subsequently converted to single-stranded siRNA. Generation of single stranded siRNA is a pre-requisite for recognition and …
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Factors That Influence Mammalian Enteric Virus Infection
RNA viruses are a common cause of emerging diseases due to their vast genetic diversity. This diversity is largely attributed to mutations generated by the error-prone viral RNA-dependent RNA polymerase during replication. Despite the ability to acquire mutations beneficial to the virus, most …
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
<p>Influenza is a segmented negative strand RNA virus. Each RNA segment is encapsulated by viral nucleoprotein (NP) and bound by the viral RNA dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also …
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