Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"RNA-binding proteins (RBPs)"”.
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Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
… of gene expression is mediated in part by RNA binding proteins (RBPs). Roles for RBPs have emerged in the immune system but remain largely unexplored. My project utilised high-throughput genetic screens to identify roles for RBPs in lymphocytes. Initially, I generated custom sgRNA libraries …
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Inferring Transcriptional and Post-Transcriptional Network Structure by Exploiting Natural Sequence Variation
… has made it possible to use messenger RNA levels as quantitative traits in linkage studies. This has created new opportunities to study genetic variation at the level of gene regulatory networks rather than individual genes. This thesis consists of four parts, each of which outlines a …
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Regulation of Pumilio RNA Binding Proteins by Long Noncoding RNA NORAD
… and encodes thousands of long noncoding RNAs (lncRNAs). Defining the mechanism of action of lncRNAs has been a critical challenge and priority for understanding their biological functions. An important example of this is the lncRNA NORAD, which is a highly conserved lncRNA that is …
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Computational analysis of cooperation and competition mechanisms between RNA-binding proteins
… regulatory trans-elements forming this network, RNA-binding proteins (RBPs) play key roles in post-transcriptional regulation of gene expression (PTR). Understanding RBPs behavior is therefore crucial given their participation in all stages of the mRNA life cycle. Although an increasing amount of …
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Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells
… assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), it remains a crucial question in the field to gain a better understanding of what specific/distinct function(s) SMN might have in motor neurons. We have identified novel interactions between SMN and two RNA-binding proteins (RBPs) …
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Development of Specific Inhibitors that Prevent hnRNPA1-Mediated Therapy Resistance in Small Cell Lung Cancer.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a diverse family of RNA-binding proteins (RBPs) that are involved in many steps in gene regulation. The hnRNP family consist of 20 members which are expressed in humans (A1 to U). The first member identified being hnRNP A1; this is a …
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Understanding regulation of mRNA by RNA binding proteins
Posttranscriptional regulation of mRNA by RNA-binding proteins plays key roles in regulating the transcriptome over the course of development, between tissues and in disease states. The specific interactions between mRNA and protein are controlled by the proteins' inherent affinities for different …
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The Role of the ORB2 RNA-binding Protein in Post-transcriptional Regulation
RNA-binding proteins (RBPs) are key components of the post-transcriptional regulatory framework and can determine the translational fate of their RNA targets. A wide variety of post-transcriptional regulatory mechanisms are employed during the early Drosophila embryo and it has been established as …
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CELF1, PTBP1, and RBFOX2-mediated alternative splicing regulation in cardiovascular diseases
Alternative splicing (AS) is dysregulated in Type 1 diabetic (T1D) and hypoplastic left heart syndrome (HLHS) patient hearts but the mechanisms responsible are unclear. Here, we provide evidence that in these patient’s hearts that dysregulation of the RNA binding proteins (RBPs) CELF1, PTBP1, and …
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Identification of RBPMS as a smooth muscle master splicing regulator via association of its gene with super-enhancers
Alternative splicing (AS) is primarily regulated by regulatory RNA-binding proteins (RBPs). It has been suggested that a small number of master splicing regulators might control cell-specific programs and these regulators could be identified via the association of their genes with transcriptional …
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Precise Temporal Regulation of Ribonucleoprotein Complexes during the Drosophila Maternal-to-Zygotic Transition
In early animal embryos, maternally loaded mRNAs and proteins regulate development prior to the handover of control to zygotically expressed gene products in a process known as the maternal-to-zygotic transition (MZT). RNA-binding proteins (RBPs) tightly regulate the translation, stability, and …
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… process. Regulation of genes transcribed by RNA polymerase II is coordinated by an elegant arrangement of protein machinery and chromatin architecture, and the resulting pre-mRNA is post-transcriptionally processed and regulated at every step to buffer, reduce, or amplify its protein output. …
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Microscopic characterization of a putative ER-membrane targeted Puf RNA-binding protein in Arabidopsis thaliana
Cellular messenger RNAs (mRNAs) are associated with RNA-binding proteins (RBPs) that form ribonucleoprotein (RNP) complexes. These RNP complexes are the functional units that participate in post-transcriptional gene regulation activities. One group of RBPs that plays an important role in regulating …
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The neuronal RNA binding protein HuB as a potential tumor suppressor in glioblastoma
… tumors, we correlated genomic alterations and mRNA levels of RNA-binding proteins (RBPs) in 372 publicly available GBM samples and identified 56 RBPs whose copy number alterations correlate with their altered expression levels in at least 15% of the samples. Among the genes identified using our …
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RNA Deregulation in Metastatic Breast Cancer
… modification of gene expression at the RNA level contributes substantially towards gain of metastatic potential. In eukaryotic cells RNA is subject to multiple layers of regulation that together generate extensive phenotypic and temporal complexity from a relatively small number of …
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Biochemical and functional characterization of human RNA binding proteins
RNA not only shuttles information between DNA and proteins but also carries out many other essential cellular functions. Nearly all steps of an RNA's life cycle are controlled by approximately one thousand RNA binding proteins (RBPs) that direct RNA splicing, cleavage and polyadenylation, …
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Investigating the Role of RNA-Binding Proteins in Tumor Response to DNA Damage-Inducing Chemotherapy
… and relapse. Recent studies indicate that RNA-binding proteins (RBPs) appear to play a particularly important role in cancer, and may contribute to chemoresistance, but the field is still at an early stage and nothing has yet progressed towards translational utility. This is at least …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
Regulated alternative splicing is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define …
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Mechanisms of Dysregulated Alternative Splicing in Cardiac and Skeletal Muscle under Diabetic Conditions
… Our lab determined a novel role for PKC in alternative splicing (AS) regulation during heart development. We found that PKC phosphorylates and controls RNA-binding proteins (RBPs) CELF1 and RBFOX2 that are involved in developmentally regulated AS in the heart. Since PKC is chronically activated …
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The evolution, modifications and interactions of proteins and RNAs
Proteins and RNAs are two of the most versatile macromolecules that carry out almost all functions within living organisms. In this thesis I have explored evolutionary and regulatory aspects of proteins and RNAs by studying their structures, modifications and interactions. In the first chapter of …
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