Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"RNA-Induced Silencing Complex"”.
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Characterization of the roles of Xrn1p in small-RNA-mediated gene-silencing pathways
RNA interference (RNAi) is a small-RNA-mediated gene-silencing pathway that is involved in viral defense, transposon silencing, heterochromatin formation, and post-transcriptional gene silencing. Most RNAi pathways are initiated by long, dsRNA which is processed by Dicer into small interfering RNAs …
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RNAi-related genes in Toxoplasma gondii.
RNA interference (RNAi) is an evolutionarily conserved mechanism through which double-stranded RNA (dsRNA) triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced …
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Application de la technique RNAi au silençage d’un rapporteur
Le système d’interférence par ARN (RNAi) a été découvert récemment. A la base, il semblerait avoir constitué un mécanisme de défense naturel de l’organisme en réponse à l’expression anormale de gènes, notamment ceux résultants de l’envahissement viral ou de transposons. A l’heure actuelle, il est …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… first successful Phase III clinical trial of a RNA interference (RNAi)-based therapeutic is a major achievement in the field. Synthetic RNAi therapeutic oligonucleotides are either first cleaved by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and …
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Building the Human Argonaute 2 Interaction Network
RNA interference (RNAi) is a system that has been largely studied and defined by its ability to affect gene expression and translation in the cytoplasm. However, Argonaute (AGO) proteins, which are the major catalytic component of the RNA-induced silencing complex (RISC), have been found to be …
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The Function and Mechanism of RNA Interference in Neurospora
RNA interference (RNAi) is a conserved gene silencing mechanism important for various biological processes, including developmental timing, genome defense, and heterochromatin formation. RNAi is triggered by double stranded RNA (dsRNA), which is processed by Dicer to siRNA. siRNA is loaded onto …
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Development and characterization of chemical modifications for siRNA strand selection
Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects one of the two strands as a guide to form the RNA induced silencing complex …
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Regulatory Mechanism of the RNAi Pathway
RNA interference (RNAi) is post-transcriptional gene silencing initiated by Dicer, a RNase III that processes double-stranded RNA (dsRNA) precursors into small interfering RNA (siRNA). In Drosophila, Dicer2 and R2D2 coordinately recruit duplex siRNA to the effector RNA-induced silencing complex …
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The synthesis and in vivo evaluation of chemically modified siRNAs that contain red-shifted internal azobenzene derivative spacers for selective and tunable photocontrol of activity without UV light
Short interfering RNAs (siRNAs) are biopolymers that are used for post-transcriptional gene regulation and act as endogenous defenses against attack from viruses and dsRNA parasites. The siRNA field is making advances for use as pharmaceuticals and biomolecular research tools for the study of gene …
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Modulation of Alphaviruses by Small RNAs
… biological transmission cycle that involves alternating virus replication in a susceptible vertebrate and mosquito host. Although infection of the vertebrate host is acute and often associated with disease, continual transmission of these viruses in nature depends on the establishment of a …
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The Mechanism of RNA Interference in Neurospora
In the canonical RNA interference (RNAi) pathway, small-interfering RNA (siRNA) duplexes generated by Dicer are incorporated into the RNA-induced-silencing complex (RISC), and subsequently converted to single-stranded siRNA. Generation of single stranded siRNA is a pre-requisite for recognition and …
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The Regulation of Microrna Biogenesis By Ribosome-Interacting Proteins
<p>MicroRNA (miRNA) are small, non-coding RNAs that affect gene expression through degradation of complementary mRNA targets or inhibition of translation. As they affect approximately 50% of all cellular processes, miRNA are tightly regulated by the cell through transcriptional and …
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RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6
… ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in …
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The Design, Synthesis, and Evaluation of Zwitterionic and Cationic Lipids for In Vivo RNA Delivery and Non-Viral CRISPR/Cas Gene Editing
… treatment of many genetic diseases. The use of RNA interference (RNAi) as a therapeutic is an exciting and rapidly developing field that offers a promising alternative to small molecule drugs for the treatment of dysregulatory diseases, including cancer. Small interfering RNA (siRNA) can be …
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The conjugation and evaluation of hydrophobic small molecules to trazole-linked siRNAs
… acid-based therapies, such as short interfering RNAs (siRNAs), have been developed to aid in the regulation of proteins. SiRNAs serve as substrates for the highly conserved RNA interference (RNAi) pathway, which gives rise to their high degree of mRNA target specificity and their catalytic …
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The Role of miR-126/126* in Microenvironmental Regulation of Cancer Metastasis
… an unbiased screen examining expression of miRNAs, and found that miR-126 and miR-126*, whose expression are regulated by methylation of the promoter of their host gene Egfl7 inside tumour cells, were significantly negatively correlated with metastatic potential. Using both mouse xenograft …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… in cancer research in the advent of the first RNA interference (RNAi) application in humans. RNAi solicits the use of a double-stranded RNA substrate, aptly named short-interfering RNA (siRNA), which binds to and triggers the degradation of a targeted complementary mRNA strand within the …
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Molecular requirements for FMRP and RNA helicase MOV10 in the translational regulation of co-bound mRNAs
… (FMRP), regulates translation of its bound mRNAs through an incompletely defined mechanism. Historically, FMRP has been known to directly associate with Argonaute (AGO), a key effector protein of the RNA induced silencing complex (RISC). MicroRNAs regulate key cellular processes in mammalian …
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Advances in molecular-based diagnostics and biomolecular interaction screening using silicon photonic microring resonators
… of a wide variety of assays quantifying DNA, RNA, and proteins, as well as the screening of more complex and subtle biomolecular interactions. Herein I describe assay developments using novel reagents and methodologies to specifically address the quantitation challenges associated with various …
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The Roles of miR-155 and miR-15/16 in Natural Killer Cell Function and Maturation
… increased risk of developing cancer.</p><p>Micro-RNAs (miRNAs) regulate numerous cellular functions. MiRNA genes are transcribed as long primary (pri-miRNA) transcripts that are subsequently 'cropped' by the Drosha/Dgcr8 complex into precursor miRNAs (pre-miRNA) that have a characteristic …