Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"RNA-FISH"”.
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Visualizing Allele Specific Expression In Single Cells
Single molecule RNA FISH techniques have enabled the quantification of gene expression at the single cell level, revealing significant variability that has been previously obscured by techniques measuring population averages. These techniques, however, have been limited in that they cannot classify …
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Using Single-Cell Analyses to Uncover Transcriptional Heterogeneity in Plasmodium falciparum
… on sex-specific expression independent of stage. RNA fluorescent in situ hybridization (RNA-FISH) validated the mutually exclusive expression pattern of male and female transcripts in gametocytes. These analyses uncovered novel male and female markers that are expressed as early as stage III …
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Characterizing slc8a4b as an Osteoblast Marker in Danio rerio
… is an emerging marker for osteoblasts in zebrafish, enabling further study of healthy bone development, bone regeneration, and skeletal diseases. To guide future use of slc8a4b as an osteoblast marker, this study aims to identify which osteoblast functions slc8a4b expression is most closely …
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Piezo1-dependent mechanical regulation of chemical signalling in the developing Xenopus laevis brain
… First, I adapted hybridisation chain reaction RNA fluorescence in situ hybridisation (HCR RNA-FISH) to Xenopus laevis brains. I then probed the expression of different chemical cues upon knockdown of the mechanosensitive ion channel Piezo1, which transduces mechanical signals into intracellular …
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Single Cell Transcriptomics and Spatial Localization in Mouse Neurons
… neuronal molecular function, I used single-cell RNA sequencing to characterize the dental primary afferent (DPA) neurons, a subset of the trigeminal ganglion that innervates the dental pulp and plays a significant role in dental hypersensitivity; I found that the DPA neurons were heterogenous and …
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Characterising functions of long non-coding RNAs in Drosophila embryogenesis
… of functional non-coding ribonucleic acids (RNAs). In recent years, the number of studies in the field of long non-coding RNA (lncRNA) biology has increased dramatically. Transcriptomic analyses of the eukaryotic genome revealed that the genome is pervasively transcribed and contains a vast …
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Post-transcriptional regulation of poly(A) binding proteins direct multiple facets of cardiac development and hypertrophy
… size in response to a variety of prolonged, external stimuli. Similar to maturational hypertrophy, cellular hypertrophy of existing cardiomyocytes is responsible for the increase in size of the heart during disease. The healthy adult heart is unique from other tissues in that the rate of protein …
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Quantitative mRNA detection with advanced nonlinear microscopy
… information on quantity and localization of key mRNA transcripts in single-cell level are critical to the assessment of cancer risk, therapy efficacy, and effective prevention strategies. While current techniques are not capable to visualize single mRNA transcript beyond the diffraction limit. In …
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Nurture is Nature: The Molecular Basis of Caregiver Genotype Effects on Larval Growth and Caste Development in the Clonal Raider Ant
… the molecular basis of this IGE, I conducted RNA-sequencing on larval and adult tissues from a cross-fostering experiment, identifying a modest set of larval genes whose expression is influenced by caregiver genotype. These included neuropeptide genes known to regulate ecdysone synthesis in …