Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"RNA translation"”.
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… central dogma of molecular biology: DNA makes RNA, RNA makes protein. At that time the central dogma could only be imagined, but over the past two decades revolutionary advances in fluorescence microscopy have now made it possible to directly image transcription and translation in living cells …
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Genetic Analysis, Electrophoretic Behavior, and in Vitro Rna Translation of a Cowpea Mosaic Virus (Severe Subgroup) Causing a Severe Disease in Soybeans
Made available in DSpace on 2014-12-14T04:37:46Z (GMT). No. of bitstreams: 1 7913636.pdf: 4919420 bytes, checksum: bb6b397ff489dc218700bf350f2be612 (MD5) Previous issue date: 1978
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… the 5 untranslated region of HIV-2 genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR splicing and the CGI in HIV-2 translation and …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… the 5 untranslated region of HIV-2 genomic RNA. Two elements of interest are the 5 UTR intron and the proposed long distance base pairing interaction between the C-box and G-box termed the CGI. This research focuses on the effects of 5 UTR splicing and the CGI in HIV-2 translation and …
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Initiation of Translation of Eukaryotic Messenger-Rna at a Unique Site Lacking an AUG Codon
The initiation of translation of eukaryotic mRNA is believed to proceed by a "scanning mechanism" where the ribosome recognizes a 5$\sp\prime$ terminal cap structure (m$\sp7$GpppN ...) and then scans down the mRNA until reaching the first 5$\sp\prime$ terminal AUG or $\sp{\rm A}\sb{\rm …
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Investigating Interferon-Inducible Antiviral Effectors Through a Virological and Evolutionary Lens
… "dominant" ISGs, IFIT1, recognizes and binds to RNAs lacking the 5' features of host mRNAs, resulting in targeted suppression of viral RNA translation. This interaction creates significant host-driven evolutionary pressure, as many viruses have evolved mechanisms to evade IFIT1 antiviral …
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RNA regulatory elements and translational control
Regulation of RNA translation is crucial for optimal allocation of resources and co-ordinated production of cellular machinery, which allows maintenance of physiological functions, response to environmental stimuli, and control of virulence to establish an infection. In this work, the translational …
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Tools for RNA and cell-free synthetic biology
… and engineering biological systems. RNA synthetic biology and synthetic minimal cells are areas where design is limited by the availability of tools to observe, program, and manipulate the systems in question. In this work I present expanded toolsets to achieve these goals. The …
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Discovery and biochemical characterization of Casl3b, a Type VI-B CRISPR-Associated RNA-Guided RNase
… of endonuclease protein(s) with targeting guide RNAs that together provide interference against foreign nucleic acids. CRISPR-Cas systems exhibit wide diversity, with the ability to target DNA or RNA, and some large single effector Class 2 Cas proteins have been harnessed for genome engineering. …
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Molecular regulation of the induction of cytochrome P-450E in the estuarine fish Fundulus heteroclitus
… hepatic P-450E content, catalytic activity, and mRNA levels in Fundulus after exposure to a single dose of BNF. In a 20 day experiment, EROD was elevated in BNFtreated animals from Day 4 through Day 20. Increases in immunodetectable P-450E showed the same trend, with low control values and at …
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Viral and Host Genetic Determinants of Hepatitis C Virus Persistence and Interferon Resistance
… (IFN)-based therapy. The development of the HCV RNA replicon system has allowed the study of persistent HCV RNA replication in tissue culture. We evaluated HCV/IFN interactions within a long-term culture system of Huh7 cell lines harboring different variants of an HCV genotype 1b subgenomic RNA …
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Tracing RNA biography: in situ transcriptome profiling by novel spatial omics technologies
… attained through the precise regulation of mRNA: its metabolism, transport, and translation within individual cells across spatial and temporal dimensions. Therefore, it is critical to methodically delineate the spatially resolved post-transcriptional regulations within transcriptomes, …
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The Interaction Between Eukaryotic Translation Initiation Factor eIF4G and 3’ Cap Independent Translation Element of Barley Yellow Dwarf Virus Is Affected by Multiple Initiation Factors
… plant viruses, BYDV contains a cap independent translation element (CITE) in the 3’ untranslated region of the viral mRNA. BTE (Barley Yellow Dwarf Virus like cap-independent translation element) is one of the well characterized CITEs. BTE mediated translation primarily depends on eukaryotic …
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The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
<p>Under normoxic conditions, eukaryotes initiate translation of RNA through eIF4E recognition of the 5’ cap. However, under cellular stress, eukaryotic translation must be initiated through a 4E-independent, or “cap-independent” mechanism, involving eukaryotic initiation factor 4G (eIF4G) binding …
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The origin and evolution of the ribosome
… elements of the ribosome, namely ribosomal RNA (rRNA) and ribosomal proteins (r-proteins) provided a deep and the most comprehensive insight possible yet into the origins and evolution of the ribosome. Using phylogenetic methods that reconstruct the evolutionary history of complex RNA and …