Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"RNA targeting"”.
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Principles of HuR-RNA targeting, interaction dynamics, and functional outcomes
… of the organizational logic of gene expression. RNA-binding proteins (RBPs) and non-coding RNAs are the regulatory molecules primary responsible for interaction with target mRNAs and thereby regulating post-transcriptional processes eventually influencing characteristics of the encoded protein. …
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Development of synthetic RNA-targeting catalysts from biostable nucleic acid analogues
RNAs are major biomolecular players in biology and disease, and are a rich source of novel drug targets for molecular medicine. However, therapeutic modulation of specific RNAs remains a significant technical challenge. Single-stranded nucleic acids can fold into complex three-dimensional …
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Characterization and application of type VI-B RNA-targeting CRISPR systems
… of nucleic acids using a single, multi-domain, RNA-guided endonuclease, have been particularly useful in this regard because they enable targeting of new sites through simple Watson-Crick base pairing rules. Recent computational studies have uncovered the existence of predicted RNA-targeting …
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Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system
RNA plays a significant role in human biology and disease, not only as messenger RNA encoding proteins but also as noncoding RNA regulating DNA, proteins, and other RNA species. Until recently, it has been challenging to target RNA in a simple, efficient manner. CRISPR-Cas (clustered regularly …
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Discovery of novel CRISPR enzymes for transcriptome engineering and human health
RNA plays important and diverse roles in biology, yet molecular tools to measure and manipulate RNA are limited. Recently, the bacterial adaptive immune system, CRISPR, has revolutionized our ability to manipulate DNA, but no known RNA-targeting versions exist. To discover parallel bacterial …
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Precise and expansive genomic positioning for CRISPR edits
… tools has been fashioned from the natural DNA/RNA-targeting ability of certain CRISPR-associated (Cas) proteins and their guide RNA, which work together to recognize and defend against infectious genetic threats. This straight-forward RNA-programmed sequence recognition by CRISPR has …
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Silencing Through Heterochromatin Formation: The Role of Piwi in Transposon Targeting
… an intriguing question. Models involving small RNAs, epigenetic inheritance of histone modifications, DNA binding proteins targeting specific motifs, and other mechanisms have been proposed.</p><p>Small RNA targeting of heterochromatin formation is an established mechanism in fission yeast: …
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Synthesis, Characterization, And Rna Reactivity Of Amino-Acid-Linked Cisplatin Analogues
<p>The essential role of ribosomal RNA (rRNA) in the cell life cycle is highlighted by protein synthesis; therefore, the ribosome is considered to be an ideal drug target. Ribosomal RNAs exhibit a high level of structural diversity. The well-known anticancer drug cisplatin was previously applied as …
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… pressure, bacteria developed a set of DNA and RNA-targeting systems to combat their invaders – bacteriophages. Fundamental research on these resistance mechanisms led to the discovery of molecular tools for genetic engineering, such as restriction enzymes, bacterial argonautes and CRISPR-Cas …
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Pathogenic Characterization and Therapeutic Development for Fiblrolamellar Hepatocellular Carcinoma
… the first systemic therapeutic for FLC, by targeting this mutation. I will show how I began by targeting the protein, which proved difficult given the similarities of the mutated protein to the wild-type protein. Then, I will discuss my work on developing an RNA-targeting therapy with …
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CRISPR/CAS9 mediated gene editing for the improvement of beef and dairy cattle
… the CRISPR/Cas9 system was paired with a guide RNA targeting the aforementioned genes to create a break in the DNA of fibroblast cells from Nelore and Angus cattle. Cells were also supplied with a 90 nucleotide single stranded-oligonucleotide repair template, which allows for homology directed …
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Evaluation of TP53 and IGHV clinical molecular prognostic testing for patients with Chronic Lymphocytic Leukaemia at Tygerberg Hospital
… of extracted total ribonucleic acid (RNA), targeting exons 2 to 11 of the entire coding region. IGHV mutational status was assessed using a two-step approach, firstly clonality was determined via fragment analysis by capillary electrophoresis of framework regions of the heavy chain …
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Lead compound discovery for myotonic dystrophy
… lead to formation of stable poly(CUG) mRNA which mislocalize splicing factors such as MBNL1 and lead to missplicing in the cell. One therapeutic strategy is to target such mutant mRNA with small molecules to prevent the sequestration of splicing factors which will prevent the multiple …
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Nuclear binding and translation regulation by FMRP through novel associated protein and putative RNA helicase MOV10
… of FMRP has not been widely studied. FMRP is an RNA binding protein that is important for normal neuronal development and maturation as well as proper cognitive development as the lack of FMRP results in fragile X syndrome (FXS). I next move out of the nucleus and study the role of FMRP and …
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Single channel studies on the modulation of connexin hemichannels by Cx43 targeting peptides and cytoplasmic Ca2+ in the context of heart disease
… signaling pathway consists of connexin targeting peptides like Gap26 and Gap27, which are identical to sequences located respectively on the first and second extracellular loop regions of Cx43. Intriguingly, despite the growing interest and wide use of connexin mimetic peptides in …
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Gene therapy for axon regeneration
… affects more than 2.5 million people (International Spinal Research Trust). Spinal cord injury often results in loss of essential bodily functions including sexual function, bladder control, and motor and sensory function. There are many factors that contribute to the failure of axon …
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THE ROLE OF CIRCULAR RNA CDR1AS IN MACROPHAGE MEDIATED CARDIAC INJURY AND REPAIR
… macrophage function during injury. Circular RNAs are newly discovered non-coding RNA generated from protein-coding genes ubiquitously expressed in mammalian tissue, highly conserved among species, and recently implicated in the possible regulation of macrophage activation. However, their role …