Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"RNA target"”.
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Single molecule studies of rna-target interactions
… techniques to study how the folding of RNA allows it to perform its various functions by recognizing and binding a target ligand. We have shown that the c-di-GMP riboswitch undergoes a large, global conformational change upon binding of the ligand. The folding dynamics of the c-di-GMP …
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Interaction of Spliceosomal U2 snRNP Protein p14 with Its Branch Site RNA Target
<p>Newly transcribed precursor messenger RNA (pre-mRNA) molecules contain coding sequences (exons) interspersed with non-coding intervening sequences (introns). These introns must be removed in order to generate a continuous coding sequence prior to translation of the message into protein. The …
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Development of Catalytic RNAs as Potential Anti-HIV-1 Agents
<p>RNA molecules with enzymatic activity (ribozymes) targeted to the genome of HIV-1 were developed and demonstrated to mediate site-specific cleavage of the HIV-1 RNA in vitro and to inhibit viral proliferation intracellularly. Two classes of ribozymes that incorporate a "hammerhead" catalytic …
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Targeting HIV-1 RNAs with Medium Sized Branched Peptides Featuring Boron and Acridine-Branched Peptide Library Design, Synthesis, High-Throughput Screening and Validation
RNAs have gained significant attention in recent years because they can fold into well-defined secondary or tertiary structures. These three dimensional architectures provide interfaces for specific RNA-RNA or RNA-protein interactions that are essential for biological processes in a living system. …
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Cas protein diagnostics for pathogen nucleic acids
… ourselves into two teams - one focusing on a DNA target and one focusing on an RNA target. The Ajioka Lab was assigned to create a test for a DNA target and we selected Hepatitis B Virus to be our test case. In my thesis, I investigate the process for producing tests using CRISPR Cas technology …
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DNA based biosensors for environmental and medical applications
… recognize selectively a complementary sequence (RNA or DNA), named target, by a hybridization reaction. Among the sequence probes, modified locked nucleic acid (LNA) and the peptide nucleic acid (PNA) were used. In this case the screen printed electrode Abstract iv was used only as transducer …
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Understanding the intrinsic antibiotic resistance in Lysobacter enzymogenes, a ubiquitous environmental bacterium
… in environmental bacteria provides new targets for discovery of novel therapeutics. However, little is known about the antibiotic resistance of the vast majority of environmental bacteria. The ubiquitous Gram-negative Lysobacter bacteria, as promising biocontrol agents, confer intrinsic …
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Functionalizing Branched Peptides with Unnatural Amino Acids Toward Targeting HIV-1 RRE RNA and Microbials
… the protein Rev with Rev Response Element (RRE) RNA is critical to the HIV-1 life cycle as this complex is required for the export of singly-spliced and unspliced mRNAs from the nucleus to the cytoplasm. Disruption of this interaction is considered to be a powerful strategy towards the …
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Expression Profiling by DNA Microarrays : Development of Amplification Methods for the Analysis of Minimal Tumor Samples
… oligonucleotides were introduced as an alternative expression profiling platform with distinct advantages to both cDNA arrays and commercial arrays produced by in situ synthesis of multiple short oligonucleotides per gene. However, gene expression analysis using microarrays of long …
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Novel RNA Targets of the Spinal Muscular Atrophy Protein
… and the spliceosome which catalyzes pre-mRNA splicing. The biogenesis of RNPs often involves complex and elaborate pathways involving post-translational modifications, transit into specific cellular domains, and several auxiliary factors including assembly chaperones. One of the …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
The fate of a bacterial RNA transcript is controlled by three key players: ribonucleases, RNA binding proteins (RBPs) and small regulatory RNAs (sRNAs). Together these form an information-rich regulatory network that exercises post-transcriptional control of gene expression. A key RBP in bacteria …
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Targeting Alternative Conformational States of the HIV-1 Rev Response Element Stem IIB using Small Molecules
<p>RNAs are growing in their importance as regulators in multiple biological processes. A deep understanding of how RNAs function within cells requires an understanding of their dynamic behavior to enable the targeting of RNA in drug discovery as therapeutics. The HIV-1 Rev response element (RRE) …