Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"RNA splicing"”.
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The evolution and specificity of RNA splicing
… excise all the introns in a process known as splicing. This excision is determined by the sequence of the gene, but in a complicated way that is not fully understood. By analyzing gene sequences we can learn about how cells decide which sequences to splice. We have developed two new …
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Development of site-specific RNA labeling strategies to probe alternative RNA splicing
… was to develop a method for the labeling of RNA at specific locations using non-natural base pairs. This thesis details the efforts made towards this objective through the development of a modular synthetic platform for an expanded genetic alphabet and its evaluation using transcriptional …
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Genetic Interactions of Factors That Regulate Alternative Rna Splicing In The Male Germ Line of Drosophila
<p>Alternative RNA splicing is a critical process that contributes variety to protein functions, and further controls cell differentiation and normal development. Although it is known that most eukaryotic genes produce multiple transcripts in which splice site selection is regulated, how RNA …
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CP1 domain of leucyl-tRNA synthetase: dissecting its dual roles in amino acid editing and RNA splicing
Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2012-06-27T21:32:36Z Item is restricted until 2014-06-27T21:32:23Z
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Characterization of Leucyl -Trna Synthetase From Escherichia Coli and Saccharomyces Cerevisiae in Aminoacylation, Amino Acid Editing, and Protein-Dependent RNA Splicing
… aminoacylation, amino acid editing, and RNA-protein interactions.
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Mechanisms of leucyl-tRNA synthetase dependent group I intron splicing
Leucyl-tRNA synthetase (LeuRS) plays dual roles within the yeast mitochondria. In addition to protein synthesis, it is also essential to RNA splicing of critical respiratory genes. The LeuRS collaborates with a maturase to excise the bI4 and aI4α introns from the cob and cox1α genes respectively. …
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Identifying Novel Causes of X-Linked Heterotaxy
… of an intronic sequence as a pseudoexon during RNA splicing. Further analysis revealed the variant profoundly altered RNA splicing, resulting in the production of several novel ZIC3 isoforms and reduced expression of normal ZIC3 protein. These novel isoforms displayed abnormal function in a …
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Serine Starvation Modulates mRNA Splicing Through Depletion of SR Proteins
… serine starvation induces dramatic changes in mRNA splicing. These effects are due, in part, to reduced translation of serine-rich proteins in serine-starved conditions, including the serine/arginine-rich splicing factor (SRSF) proteins that are known regulators of mRNA splicing. Indeed, we have …
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Quantitative isoforrm profiling & isoform convergence
Alternative pre-messenger RNA splicing is a crucial step in eukaryotic gene expression, and therefore it is subject to tight regulation. Given its importance in conferring protein diversity, alternative splicing is sensitive to changes in cellular states including malignancy. We present a new …
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
The RNA recognition motif (RRM) is the most abundant RNA binding domain that is found in all organisms. RRM-containing proteins participate in most steps of gene expression, including translation, splicing, modification and transport of RNA. This dissertation aims to help understand the …
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New Biological Pathways
… information storage, I propose and develop trans-splicing as a strategy to barcode the introduction of genetic elements en masse, and show that cell transcriptomes can be reliably barcoded for facile information storage. For information editing, I propose and develop a new RNA splicing machine – …
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Biochemical and Structural Studies of the Role of FKBP(L)s in the fly and Mammalian Circadian Cycle
… include protein folding, molecular trafficking, RNA splicing and cell cycle regulation among others. Bride of Doubletime (BDBT) previously designated as CG17282 in the flybase was identified as a circadian clock component that stimulates DBT’s circadian function. Here, we have used biochemical …
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Lessons from a rare disease
… such as gene expression, DNA regulation, RNA splicing, protein processing, cellular aging, and stem cell differentiation. Instrumental to the discovery of the progeria gene and the growth of scientific interest since 2002 has been The Progeria Research Foundation.
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Structures and Regulation of the N6-Methyladenosine Methyltransferase METTL16
Chemical modifications of RNA control the abundance and function of both coding and non-coding RNA molecules. The most abundant mRNA modification is N6 methyladenosine (m6A), which has been shown to regulate RNA splicing, translation and decay. The m6A modification is deposited by …
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Studies of exon scrambling and mutually exclusive alternative splicing
… this thesis work were to study two special alternative splicing events: exon scrambling at the RNA splicing level and mutually exclusive alternative splicing (MEAS) by computational and experimental methods. Chapter 1 presents work on the study of exon scrambling, in which exons are spliced at …
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Polymorphisms in the Flt1 gene and their relation to expression of the secreted Flt1 variant
… form of the receptor (sFlt1) translated from an mRNA in which a portion of intron 13 is preserved. sFlt1 retains high affinity for VEGF and thereby inhibits its angiogenic activity. Intron 13 contains important cis elements involved in sFlt1 mRNA formation. Here, we test the hypothesis that …
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Computational methods for splice site prediction
… are removed from the primary transcript during RNA splicing, the exons are the coding regions that are spliced together during the mRNA maturation process. The borders between exons and introns present conserved dinucleotides, and are called splice sites; the intron-exon boundary is referred to …
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Single-cell transcriptomics identifies key components in metabolic pathways of drug perturbed activated CD4+ T cells
… and the effector status of cells. Leveraging RNA splicing information, I demonstrate that expression changes of genes targeted by compounds resulted in altered T cell lineage development. Furthermore, my analysis highlights important transcription factors and metabolic pathways regulated by …
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