Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"RNA silencing"”.
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P1/HC-Pro aus dem Weizenstrichelmosaikvirus als Suppressoren des RNA silencing in Weizen
… die Expression eingebrachter Gene durch RNA silencing vermindern zu können. In dieser Arbeit wurde untersucht, ob die Expression von Transgenen und möglicherweise auch die Transformationseffizienz (d.h. Anzahl transgener Pflanzen pro verwendetem Explantat) in Weizen durch Suppressoren des …
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The Role of Endogenous Arabidopsis Proteins In Rna Silencing and Hc-Pro-Mediated Suppression of Silencing
<p> RNA silencing is a sequence-specific RNA degradation pathway that acts as an antiviral defense pathway. Viruses have evolved suppressors of RNA silencing, including the potyviral protein Helper Component-Proteinase (HC-Pro). Our focus on evaluating the mechanism of HC-Pro function has led us to …
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Biochemical and cell biological analysis of the mechanism of RNA interference in human cells
RNA-Interferenz (RNAi) wird in menschlichen Zellen durch kurze, aus 19-24 Basenpaaren bestehende, interferierende RNA (siRNA) Duplexe ausgelöst, welche die doppelsträngigen Vorläufer der endogenen microRNAs (miRNAs) imitieren. Einzelsträngige siRNAs und miRNAs werden in Argonaute-Proteine …
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Small RNAs in Tomato: from defence to development
RNA silencing is a major regulator of gene expression in plants, controlling from development to transposable element silencing and stress responses. As part of the silencing machinery, micro (mi)RNAs orchestrate silencing of their targets, either directly or through cascades of secondary small …
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Modulation of Alphaviruses by Small RNAs
… biological transmission cycle that involves alternating virus replication in a susceptible vertebrate and mosquito host. Although infection of the vertebrate host is acute and often associated with disease, continual transmission of these viruses in nature depends on the establishment of a …
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Maize streak virus (MSV) diversity in Uganda and the assessment of gene silencing as a tool for development of resistance to MSV
… this study also included an assessment of RNA silencing as a resistance strategy against MSV.
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Engineering resistance to maize lethal necrosis
… resistant maize lines using an artificial microRNA (amiRNA) approach. MLN is a synergistic viral disease caused by the interaction of Maize chlorotic mottle virus (MCMV) with any maize-infecting member of the potyviridae. I used next-generation RNA sequencing to characterise the MLN outbreak in …
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Small RNA and genome interactions in Chlamydomonas reinhardtii recombinants
… in the formation of transgressive phenotypes. RNA silencing is an epigenetic mechanism of gene regulation relying on 20Q24nt single stranded small RNAs (sRNAs). Small RNAs, due to their ability to set up persistent epigenetic marks at a locus, have the potential to create heritable …
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Sequence-specific gene silencing by transitive signals in plants
… field that aims at unraveling the process of RNA silencing, in particular of transitive silencing. In the first, application-oriented part of the thesis, we examined the potential of transitive silencing as a tool for functional genomics, and in the second, more fundamental part, we focused on …
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of …
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Restoring and enhancing Argonaute2-catalyzed cleavage
The core of the RNA silencing pathway relies on an Argonaute protein in complex with a small RNA. Together, this complex targets transcript RNAs through sequence complementarity to induce the destruction of the transcript RNA either through Argonaute2-mediated slicing or mRNA destabilization and …
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Molecular Genetic Analysis of Non-Catalytic Pol IV and V Subunits
… have the distinction of encoding multisubunit RNA polymerases used exclusively for RNA directed DNA Methylation: RdDM) in addition to Pol I, II, and III. In Arabidopsis thaliana, Pol IV is required for the biogenesis of 24nt siRNAs whereas Pol V transcription is needed for cytosine methylation …
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Genetic and molecular analysis of <em>drop out</em>, the single homolog of the vertebrate MAST kinases in <em>Drosophila melanogaster</em>
… provided evidence that dop1 is an allele of the RNA silencing gene argonaute2 (ago2). However, results presented in this thesis showed that ago2 functions are unimpaired in dop mutant embryos using genetic and biochemical tools. Moreover genetic and molecular mapping revealed that dop mutants …
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Interactions between Cucumber mosaic virus proteins and host proteins
… into host cells. The 2b viral suppressor of RNA silencing (VSR) encoded by CMV is a potent counterdefence and pathogenicity factor that inhibits antiviral silencing by titration of short double-stranded RNAs. The 2b VSR not only influences infection, but also host interactions with one of the …
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Characterization of Inducible SMC-specific Drosha Knockout Mice
… cells. Recent studies have shown that microRNAs (miRNAs), a new class of small RNAs that function as guide molecules in RNA silencing, regulate and maintain the differentiation state of SMCs. A transgenic animal model to study SMC-specific knockout of Dicer, one of two RNase III proteins in …
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Regulation of exogenous retroviruses and endogenous retroelements by MOV10
… genetic parasites. Human MOV10 is a putative RNA helicase with inhibitory or stimulatory roles in the replication of several RNA viruses, and the homologs of which play vital roles in the restriction of viruses and endogenous retroelements. Furthermore, MOV10 interacts with antiviral APOBEC3 …
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Role of Trp Channels In Mediating The Calcium Signaling Response of Brain Endothelial Cells to Mechanical Stretch
… of the stretch-induced Ca<sup>2+</sup> influx. RNA silencing directed at individual TRP channel subtypes revealed that TRPC1 and TRPP2 largely mediate the stretch-induced Ca<sup>2+</sup> response. In addition, we found that nitric oxide (NO) levels increased as a result of mechanical stretch, …
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SYSTEMIC AND TRANSGENERATIONAL REGULATION OF GENE EXPRESSION BY SMALL RNAS IN C. ELEGANS
… (transgenerational regulation). Double- stranded RNA (dsRNA) introduced to an animal can silence a gene of matching sequence within that animal and this silencing can persist in progeny suggesting that RNA has the potential to transfer gene-specific regulatory information. Using RNA silencing in …
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Discovery and biochemical characterization of RNA interference in budding yeast
RNA interference (RNAi) is a eukaryotic pathway for the post-transcriptional regulation of gene expression. In the simplest form of RNAi, a double-stranded RNA (dsRNA) trigger is converted into small-RNA duplexes by the Dicer enzyme. These duplexes are then loaded into the effector protein …
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