Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"RNA polymerase (RNAP)"”.
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Mechanism of Transcription Arrest By The Nun Protein of Bacteriophage HK022
… (Roberts et al, 2008). Escherichia coli RNA polymerase (RNAP) and its auxiliary transcription factors have long been studied as a model for understanding regulation of elongation, but biochemical insights into some of these mechanisms still must be elucidated. In this study I explored the …
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Characterization of the Omega Subunit of Borrelia Burgdorferi’s RNA Polymerase
… changes in gene expression carried out by RNA polymerase (RNAP). The RNAP holoenzyme consists of two ɑ subunits, a β subunit, and a β’ subunit. Additional proteins have been implicated in the function of RNAP including RpoZ (i.e. omega subunit, ω). Investigations of RpoZ homologues from …
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Effective interactions arising from resource limitations in gene transcription networks/
… for transcription and translation, such as RNA polymerase (RNAP) and ribosomes, which are found in cells in limited amounts. As various genes in a network compete for a common pool of resources, a hidden layer of interactions among genes arises. Such interactions are not reflected by …
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Structural and Functional Studies of the Unconventional Proteobacterial Transcription Activator Crl in Complex with the Transcription Machinery
<p>The σ<sup>S</sup> subunit of RNA polymerase (RNAP) is the master regulator of stress responses in many Gram-negative bacteria. This alternative σ factor assembles with the core RNA polymerase to initiate the transcription of genes needed to survive different environmental changes. Crl is a small …
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New mechanisms in transcription regulation and chromosome organization in Caulobacter crescentus
… transcription factors, that interact with DNA or RNA polymerase (RNAP) or both, and can change the preference of RNAP for target promoters. Additionally, DNA is tightly compacted and organized inside cells. In bacteria, nucleoid-associated proteins (NAPs) play critical roles in shaping and …
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Structural Analysis of an RNA Polymerase Sigma Factor and Its Anti-sigma: sigma28/FLGM
… regulation, sigma is a dissociable subunit of RNA polymerase (RNAP), and sequence-specifically recognizes promoters, but only after associating with RNAP to form the holoenzyme. In the holoenzyme, the major sigma domains (sigma2, sigma3, and sigma4) are spread across the surface, with the …
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Structural and Computational Studies of RNA Polymerase
<p>Although the multi-subunit RNA Polymerase (RNAP) structures have revolutionized our understanding of transcription, we still do not fully understand the molecular details of bacterial promoter recognition and melting. In addition, our understanding is generally limited to highly conserved …
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Structural and Functional Studies of Mycobacterial General Transcription Factors RbpA and CarD
… kinetic studies have used Escherichia coli (Eco) RNA polymerase (RNAP) as a model for studying prokaryotic transcription initiation. However, properties of Eco RNAP are not representative of RNAPs from other bacterial species. Transcription in mycobacteria is distinct from Eco by its formation of …
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Going Beyond Phosphorylation: Acetylphosphate-Mediated Ran Polymerase Acetylation and Caper Phosphorylation Co-Regulate Transcription of Escherichia Coli Stress-Responsive Gene cpxP
… modification, Nε-lysine acety-lation of RNA polymerase (RNAP). My data implicate the acetylation of two lysine side chains on the carboxyl terminal domain of the α subunit (αCTD) of RNAP in the modulation of cpxP transcription in response to the AcP-dependent phosphorylation of CpxR. …
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… capable of making multiple contacts with RNA polymerase (RNAP). The specific role of region 4 of the Escherichia coli sigma 70 subunit of RNAP in LuxR-dependent transcriptional activation of the luxI promoter has been investigated. Rich in basic amino acids, this conserved portion of sigma …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… contacts with the alpha and sigma subunits of RNA polymerase (RNAP). Published work describing the results of alanine scanning mutagenesis performed on the C-terminal domain of LuxR (residues 190-250) has identified residues (K198, W201 and I206) that appear to play a role in positive control …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… activator making multiple contacts with RNA polymerase (RNAP). The specific role of the <font face = "symbol">a</font>CTD of RNAP in LuxR-dependent transcriptional activation of the <i>lux</i> operon promoter has been investigated. The effect of seventy alanine substitution variants of …
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Structure determination of bacteriophage [phi]21 N-peptide and boxB RNA complex by NMR spectrocsopy
… E. coli bacteriophage interacts with the nut RNA site and host factors to transform RNA polymerase (RNAP) into a termination resistant transcription complex. The [delta], P22 and [phi]21 phage N proteins share a conserved amino-terminal, arginine-rich region, which confers phage-specific …
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Factors and mechanisms of archaeal transcription termination and DNA repair
RNA synthesis by RNA polymerase (RNAP) is an essential process and must be properly regulated both temporally and spatially to ensure cellular health in dynamic environments. Regulation of RNA synthesis in response to internal and environmental stimuli is typically achieved through interactions …
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A model for sigma factor competition in bacterial cells
… such as sigma factors. They reversibly bind the RNA polymerase to form the so-called holoenzyme and direct it towards the appropriate stress response genes. In exponentially growing E. coli cells, the majority of the transcriptional activity is carried out by the housekeeping sigma factor, while …
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Characterization of major virulence regulators of Erwinia amylovora
… the transcription initiation level and its core RNA polymerase (RNAP) requires sigma factors for promoter recognition and initiation. In this study, we investigated the role of several sigma factors in regulating virulence gene expression in E.amylovora. Early studies have shown that hrp-type III …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… associated genes (cas) encode an adaptive, small-RNA-based immune system that protects prokaryotes from infectious phages and plasmids. CRISPR-Cas systems can be classified into three types based on their cas gene content. My thesis work focused on two parts. First, I investigated the mechanism …
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A Structural Perspective on Bacterial Transcription
… is a multistep process. In the first step, RNA polymerase (RNAP) contacts with the promoter DNA are established and then must be broken for the enzyme to transition into the elongation phase of RNA production; a process known as promoter escape. While single-molecule and biochemical …
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… box) or to establish associations with RNA polymerase (RNAP) at the <I>lux</I> operon promoter necessary for transcriptional initiation. A third <I>in vivo </I>assay was used to test the ability of the altered forms of LuxR to bind to the <I>lux</I> box (DNA binding assay/repression). …
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Die Regulation der prokaryontischen Transkription auf molekularer Ebene
In Escherichia coli wird die RNA-Synthese durch eine aus fünf Untereinheiten bestehende RNA Polymerase (RNAP) katalysiert. Für die Bildung der Phosphodiesterbindung in der RNA sind die beta- und beta’-Untereinheiten verantwortlich. Die aminoterminalen Domänen der beiden alpha-Untereinheiten sind …
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