Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 118 for “"RNA molecules"”.
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A therapeutic approach to chronic myeloid leukaemia using short hairpin RNA molecules
… method of treatment for CML through the use of RNAi to abolish the increase in tyrosine kinase activity of the abnormal fusion gene product.<br/>The K562 and KCL22 cell lines incorporating the β3α2 and β2α2 fusion points respectively were used in this project. The fusion points were cloned and …
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Aminormotiffinder - a graph grammar based tool to effectively search a minor motifs in 3D RNA molecules
RNA Motifs are three dimensional folds that play important role in RNA folding and its interaction with other molecules. They basically have modular structure and are composed of conserved building blocks dependent upon the sequence. Their automated in silico identification remains a challenging …
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Bioinformatics approaches to analysing RNA mediated regulation of gene expression
… stored genes into functional proteins, RNA – a third major molecule class – was discovered early on to act a messenger by copying the genomic information and relaying it to the protein-synthesizing machinery. Furthermore, RNA molecules were identified to assist in the assembly of amino …
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Using Domination to Analyze RNA Structures.
<p>Understanding RNA molecules is important to genomics research. Recently researchers at the Courant Institute of Mathematical Sciences used graph theory to model RNA molecules and provided a database of trees representing possible secondary RNA structures. In this thesis we use domination …
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Single molecule pull-down of viral RNA-protein complexes
Protein and RNA molecules interact with multiple protein partners to perform essential cellular processes such as post-transcriptional regulation of mRNA. Recently, a single molecule pull-down (SiMPull) assay was developed to isolate and study single protein complexes directly from cell lysates. …
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Nanoscale biomolecular mapping in cells and tissues with expansion microscopy
… that uses ExM to enable the nanoscale imaging of RNA throughout cells and tissues. A novel chemical approach covalently retains endogenous RNA molecules in the ExM hydrogel. After expansion, RNA molecules can be interrogated with in situ hybridization. ExFISH opens the door for the investigation …
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Anomalous diffusion of mRNA in the cytoplasm of HeLa cells
Information about the diffusive motion of RNA would provide insights into intracellular structures and functions, as well as gene expression and genetic regulation. We study the motion of individual messenger RNA molecules in the cytoplasm of HeLa cells. RNAs are imaged in live cells via total …
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Statistical thermodynamics of virus assembly
… of HIV-1 capsids. Secondly, the problem of RNA genome packaging inside spherical viruses is studied using RNA condensation theory. For weak adsorption strength of capsid protein, most RNA genomes are located at the center of the capsid. For strong adsorption strength, RNA genomes peak near …
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CAG repeat expansions induce cytoplasmic RNA aggregation
… ‘CAG’ motifs can result in disease. Mutant RNA molecules harboring an expanded repeat tract can promote pathology through at least two activities. First, these repeat-containing RNA molecules are prone to condensation within the nucleus. This results in the formation of liquid-like nuclear …
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Thermodynamics and kinetics of antisense oligonucleotide hybridization to a structured mRNA target
… strong function of the structure of the target mRNA. Though single-stranded, RNA molecules are typically folded into complex three-dimensional structures, formed primarily by intramolecular Watson-Crick base-pairing. If an oligonucleotide is complementary to a sequence embedded in the three …
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The use of genetic materials, RNA, to determine the age of saliva stains
… of time and the degradation rate of specific RNA molecules were assessed to find the correlation between them. Two types of approaches and two types of RNA molecules were used for this purpose. In the first approach, the relationship assessment was carried out between the saliva's age and the …
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MicroRNA Expression Signature of Retinal Pigmented Epithelial Cells Associated with the Phenotype of Age-Related Macular Degeneration
… (RPE) and then the photoreceptors. MicroRNAs (miRNAs) are a group of endogenous noncoding RNA molecules that originate from parts of the genome that were originally speculated to be “junk” DNA. However, it is now known that these small RNA molecules are responsible for gene silencing on …
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Studies of Plant Viroid RNA and Other RNA Species of Unusual Function
… dissertation concerns studies of plant viroid RNA and other RNA species of unusual function. By "RNA species of unusual function" we mean RNA species which do not function as transfer, ribosomal or messenger RNA. In the studies to be reported here we have laid the experimental and theoretical …
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Study of macromolecular interactions using computational solvent mapping
… that similar binding hot spots also occur in DNA molecules and interact with small molecules that bind to DNA with high affinity. Solvent mapping results on B-DNA, with or without Hoogsteen (HG) base pairing, have revealed the significance of "HG breathing" on the reactivity of DNA with …
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Site Directed Mutagensis of Bacteriophage HK639 and Identification of Its Integration Site
… antitermination is mediated by a phage-encoded RNA molecules. Recent results suggest that another bacteriophage called HK639 also appears to use RNA-mediated antitermination. To characterize this newly identified phage we generated site directed mutations and identified where the phage …
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The tree alignment model : algorithms, implementations and applications for the analysis of RNA secondary structures
… not sequential? There are numerous examples of RNA genes and motifs where the structure instead of the sequence determines the function (and for sure, there are a lot of unknown ones today). In this case the selective pressure acts on the structure, which conserves structure instead of sequence. …
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Chemical synthesis of glycosylated oligoribonucleotides for siRNA development
… methodology for the preparation of carbohydrate-RNA conjugates was established, which involved the use of 3,4~diethoxy-3-cyclobutene-l,2- dione (diethyl squarate) as the linking reagent. First, a glycan moiety containing an amino group reacted with diethyl squarate to form an activated glycan, …
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The regulation of RNA polymerase III transcription by protein kinase CK2
… depends primarily on the rate of translation. RNA polymerase (pol) III plays a key role in protein synthesis by catalysing the production of small, untranslated RNA molecules such as transfer (tRNA) and 5S ribosomal RNA (5S rRNA). Indeed, recent evidence suggests that tRNAiMet production is …
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High intensity exercise induced regulation of the microRNA biogenesis pathway
… reticulum. Recent studies suggest that microRNAs (miRNA) – small noncoding RNA molecules that fine-tune gene expression by silencing mRNAs through translational repression and/or transcript destabilization – dynamically respond to exercise and regulate the expression of genes involved in …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… methodologies for predicting protein-RNA binding affinities and designing RNA sequences with specific functional outcomes. The research introduces PANTHER (Protein-Affinity for Nucleic Target-binding, Hybridization, and Energy Regression), a novel machine learning-based scoring …
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