Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"RNA modification"”.
-
Biochemical and Structural Studies of RNA Modification and Repair
"""RNA Repair"" is a mechanism that rectifies purposeful breaks in tRNAs and mRNAs occurred during RNA processing and under cellular stress. To date, only two repair-like and repair pathways have been identified: yeast-type and phage-type, respectively. In our studies, we identified a bacterial RNA …
-
INTERROGATING THE ROLE OF N6-METHYLADENOSINE RNA MODIFICATION ON TELOMERASE BIOLOGY
… DNA of chromosomes lost during replication. The RNA subunit of telomerase, TERC, hTR acts as a template for its reverse transcriptase activity and as a scaffold for the binding of proteins involved in the maturation, stabilization, and trafficking of the telomerase holoenzyme. Mutations or …
-
Function and Mechanism of Alkbh5 In N6-Methyl-Adenosine Rna Modification In Glioblastoma
… (m<sup>6</sup>A) is the most prevalent internal chemical modification of mRNAs in eukaryotes. In mammals, m<sup>6</sup>A installed by m<sup>6</sup>A methyltransferases METTL3 and METTL14 is erased by two members of the AlkB family of nonheme Fe(II)/a-ketoglutarate (a-KG)-dependent …
-
Investigations into the role of RNA modification in the function of the c-Myc IRES
… pathways are used for the translation of mRNAs required as part of the cell stress response/permit cell survival, and internal ribosome entry segments (IRES) have been shown to recruit the mRNAs to ribosomes directly in these conditions. It is known that the majority of cellular IRESs, …
-
Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
… were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA molecule (acceptor) to form a phosphodiester bond and the AMP portion is released. E. coli and D. melanogaster 5S RNA, yeast …
-
Are Post-Transcriptional RNA Modification Events in the Glutamate-Gated and GABA-Gated Chloride Channels of Anopheles minimus and Anopheles dirus associated with Ivermectin Insensitivity?
… and bioinformatic techniques such as DNA/RNA extraction, PCR amplification and DNA/RNA sequence analysis of the GluCl and RDL channels, this study aimed to elucidate any differences in DNA and RNA sequences in the mosquito species Anopheles minimus and Anopheles dirus and their possible …
-
Role of m<sup>6</sup>A RNA "reader" YTHDF2 in Mammalian Germ Cells and Early Embryo Development
RNA modifications affect the processing and metabolism of mRNA which ultimately leads to changes in gene expression. Regulation of gene expression is essential for every tissue and every organism, specifically in cell fate determination. N6-methyladenosine (m6A) is one of the most abundant and …
-
Characterisation of RNA modifications in human cancer cells
Chemical modifications have been studied as key modulators of RNA biology for over 50 years. There are now 171 RNA modifications that have been discovered throughout the three kingdoms of life1. These chemical changes overlay the standard RNA information and have been linked to a wide range of …
-
Characterizing Modified Nucleosides in RNA by LC/UV/MS
<p>Posttranscriptional nucleoside modification is an important characteristic in all types of ribonucleic acids (RNA) and increases the functional diversity of RNA, exceeding that of the four canonical bases. In ribosomal RNA (rRNA) and transfer RNA (tRNA) these modifications enhance RNA structure …
-
Elucidating The Direct Role of M6A In The Human Transcriptome
<p>The best characterized and most abundant internal RNA modification is the methylation of adenosine at position 6 to give N6-methyladenosine (m6A). m6A modification on cellular RNAs was discovered in the 1970s. The past decade has witnessed major progress that illustrated important roles of this …
-
Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
… of gene expression is mediated in part by RNA binding proteins (RBPs). Roles for RBPs have emerged in the immune system but remain largely unexplored. My project utilised high-throughput genetic screens to identify roles for RBPs in lymphocytes. Initially, I generated custom sgRNA libraries …
-
Acing nucleic acids and chemistry : exogenous RNA/DNA acetylation and modeling universal data formats.
N4-acetylcytidine (ac4C) is an epitranscriptomic RNA modification formed by the acetylation of the primary amine of cytosine by an N-acetyltransferase with acetyl-CoA as the acetyl donor. This highly conserved modification is involved in RNA stability and translation efficacy. The natural …
-
Genome wide search for pseudo knotted non-coding RNAs
Non-coding RNAs (ncRNAs) are the functional RNA molecules that are involved in many biological processes including gene regulation, chromosome replication and RNA modification. Searching genomes using computational methods has become an important asset for prediction and annotation of ncRNAs. To …
-
Inosine-containing mRNA induces an innate immune response and is translated with lower efficiency
… of adenosine by adenosine deaminases acting on RNA (ADAR). ADAR editing activity is known to play a key role in modulating the host cell’s immune response to RNA. Here, we specifically study the effect of the presence of inosine in RNA by generating an inosine-containing reporter mRNA sequence. …
-
To the Frontier of RNA Glycosylation: Novel Insights from Metabolic Labelling
The recent discovery of glycosylated RNA (glycoRNA) has redefined our comprehension of the glyco-conjugate universe, uncovering an unprecedented link between glycans and RNA. The pioneering exploration of this novel biopolymer was driven by the metabolic carbohydrate reporter, per-O-acetylated …
-
CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
N6-methyladenosine (m6A) is the most abundant internal RNA modification of mRNA in mammals. The m6A -pathway comprises numerous effectors, which are classified based on their function. m6A is deposited co-transcriptionally by “writers”, including methyltransferase-like 3 (METTL3) and …
-
Regulation of Metabolic Stress-Induced snoRNAs
… previously unsuspected role for small nucleolar RNAs: snoRNAs) as critical mediators of lipotoxic cell death.</p><p>Herein we show that under lipotoxic conditions, intronic snoRNAs in the <italic>rpL13a</italic> gene accumulate in the cytosol during metabolic stress, suggesting that these …
-
Car-Modified T Cells Capable of Distinguishing Normal Cells From Malignant Cells
… target tumor-associated antigen (TAA) by genetic modification to express a chimeric antigen receptor (CAR), which fuses the specificity derived from an antibody to T-cell activation domains to result in lysis of TAA-expressing cells. Due to the potential for on-target, off-tissue toxicity, …
-
The role of RNA modifications in mitochondrial translation
… the mitochondrial ribosome. Post-transcriptional RNA nucleotide modifications have been identified in mitochondrial (mt)-rRNAs. However, little is known about their role in terms of mitochondrial translation and/or ribosome assembly. The mitochondrial methyltransferase MRM2 modifies the human 16S …
-
Transcriptome-wide pseudouridine profiling reveals modification of critical E. coli mRNAs
Pseudouridine (Ψ) is an ubiquitous RNA modification, present in the tRNAs and rRNAs of species across all domains of life, and is now known to be present in the mRNAs of diverse eukaryotes. However, the modification was yet to be identified in bacterial mRNAs, and its functional roles remain …
Page 1 of 2