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Showing 1 to 15 of 15 for “"RNA localization"”.
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RNA Localization and Translational Regulation on the Endoplasmic Reticulum
<p>mRNA localization is emerging as a critical cellular mechanism for the spatiotemporal regulation of protein expression and serves important roles in oogenesis, embryogenesis, cell fate specification, and synapse formation. Signal sequence-encoding mRNAs are localized to the endoplasmic reticulum …
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A role for RNA localization in the human neuromuscular disease myotonic dystrophy
RNA localization, a regulated step of gene expression, is fundamentally important in development and differentiation. In multidisciplinary experiments, we discovered that RNA (mis)localization underlies the human disease myotonic dystrophy (DM). DM, the most prevalent adult muscular dystrophy, is …
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The role of RNA localization and translational regulation in Drosophila germ cell determination
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Design and synthesis of probes for detection of protein-protein interaction and RNA localization
The use of the ketone biotin - benzophenone-biotin hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB p50 dimers was assessed. A series of benzophenone-based probes were synthesized and tested for photocrosslinking activity to investigate the efficiency of …
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Cell-Free Formation of RNA Granules
Asymmetric RNA localization is a mechanism by which a cell can spatially and temporally regulate the translation of RNAs. This mechanism is essential for many developmental processes such as germ cell formation in Drosophila embryos, as well as establishment of cell polarity and synaptic plasticity …
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Transcriptome-wide organization of subcellular microenvironments revealed by ATLAS-Seq
Subcellular localization of RNAs is a ubiquitous and evolutionarily conserved process that provides an additional layer of transcriptome organization promoting coordinated control of gene expression in both space and time. It has been shown to contribute to processes ranging from cell fate …
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
The spatial organization of RNA within cells is crucial for the regulation of a wide range of biological functions, spanning all kingdoms of life. However, a general understanding of RNA localization has been hindered by a lack of simple, high-throughput methods for mapping the transcriptomes of …
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Biochemical purification and functional characterization of the She RNP complex from S. cerevisiae
Asymmetric mRNA localization is a widely used mechanism to sort cell fate determinants in development. In the budding yeast Saccharomyces cerevisae ASH1 (for 'asymmetric synthesis of HO') mRNA localization to the tip of the growing bud leads to targeting of Ash1p to the daughter cell nucleus prior …
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Spatially precise in situ transcriptomics in intact biological systems
… disease. Current methods for highly multiplexed RNA imaging are limited in their spatial resolution and lack precise subcellular landmarks, limiting the ability to localize transcripts to nanoscale and subcellular compartments. Here, I describe the development of targeted expansion sequencing …
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A proteomic approach for studying LTR retrotransposon host factors in saccharomyces cerevisae
… in the nucleus with the transcription of Ty RNA. Following translation of Ty proteins, virus-like particles (VLPs) assemble in the cytoplasm. VLPs contain Ty RNA and enzymes surrounded by a capsid shell. Specific cellular tRNAs are also packaged within VLPs and serve as primers for reverse …
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Sequence determinants of pri-miRNA processing
MicroRNAs (miRNAs) are short RNAs that regulate many processes in physiology and pathology by guiding the repression of target messenger RNAs. For classification purposes, miRNAs are defined as ~22 nt RNAs that are produced by the cleavage of endogenously transcribed hairpins. From a cellular …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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Dynamic relocalization of maternal transcripts during oocyte meiotic maturation
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Identifizierung und funktionelle Charakterisierung neuer RNA-Transportfaktoren in der Xenopus laevis Oozyte
Die Lokalisierung von RNA-Molekülen ist ein wichtiger Mechanismus zur Schaffung intrazellulärer Asymmetrien und spielt eine bedeutende Rolle in der Embryogenese verschiedener Organismen. Die Aufklärung dieses Prozesses, sowie die funktionelle Analyse lokalisierter RNAs können folglich zum …
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Identifizierung neuer vegetal lokalisierter RNAs in der Xenopus laevis Oozyte und deren funktionelle Charakterisierung
Am vegetalen Pol der Xenopus laevis lokalisierte mRNA Moleküle haben eine wichtige Funktion während der frühembryonalen Entwicklung. Mit dem Ziel, neue vegetal lokalisierte mRNA Moleküle zu isolieren, wurden 10000 cDNA Klone einer mit vegetal-kortikal lokalisierten Transkripten angereicherten cDNA …