Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"RNA knockdown"”.
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RNA Knockdown of the Immune Checkpoint VISTA Promotes Tumor Regression
… we used Antisense oligonucleotides (ASOs) to knockdown VISTA expression on myeloid cells. ASOs are synthetically generated single stranded oligonucleotides that alter messenger RNA to either diminish, recover, or adapt protein expression. We hypothesize that reducing VISTA expression on …
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Role and regulation of mitochondrial permeability transition in cell death
… cytochrome c leading to apoptosis. Interference RNA knockdown of Bax blocked OPA1 and cytochrome c release after THG treatment, but did not prevent the MPT showing that Bax was essential for cytochrome c release by MPT. In isolated mitochondria, MPT led to OPA1 and cytochrome c release …
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The regulation of mouse embryonic stem cell differentiation by Nrf2
… by pharmacological activator, short hairpin RNA knockdown, and CRISPR-Cas9 genome editing showed that Nrf2 could promote pluripotency and inhibit differentiation, especially during early differentiation toward neural lineage. This study suggested a new player in transcription control that …
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Development of synthetic RNA-targeting catalysts from biostable nucleic acid analogues
RNAs are major biomolecular players in biology and disease, and are a rich source of novel drug targets for molecular medicine. However, therapeutic modulation of specific RNAs remains a significant technical challenge. Single-stranded nucleic acids can fold into complex three-dimensional …
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Eukaryotic protein mimicry as an infection strategy for Ehrlichia chaffeensis
… and surface plasmon resonance (SPR). Interfering RNA knockdown of Wnt receptors, coreceptors, and signaling pathway components significantly reduced E. ch. infection demonstrating complex and redundant interactions are involved in Wnt pathway exploitation. We utilized in silico approaches to …
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Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
Complementary short interfering RNAs (siRNAs) are routinely used to knockdown gene expression. siRNAs bind to their target sequence and guide transcript cleavage and subsequent degradation. This type of silencing is associated with equivalent levels of RNA and protein knockdown. siRNA-mediated …