Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"RNA isolation"”.
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Techniques of RNA Isolation and Characterization in Infectious Pancreatic Necrosis Virus-Infected Chinook Salmon Embryo Cells
… This prototype naked, double-stranded RNA virus of the Birnaviradae family was studied to test new techniques for isolation and characterization of its ambiguous early post-infection viral RNA products. RNA was extracted by either the classical phenol method or a new kit prepared by …
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Insulin-like growth factor-I in growing horses and RNA isolation from small articular cartilage samples
… rotor-stator homogenizer produced quanitfiable RNA yields, and was used to evaluate three different RNA isolation protocols. Two of the protocols were commercially available RNA extraction kits, with the third a modified guanidinium isothiocyanate/acid-phenol extraction procedure. The combined …
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BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF RAFFINOSE FAMILY OLIGOSACCHARIDE (RFO) BIOSYNTHESIS IN LENTIL (Lens culinaris Medik.)
… necessitated an optimized method to extract RNA with high purity in adequate quantities. Previously used RNA isolation procedures, namely (i) Trizol based RNA isolation; (ii) Phenol guanidine based RNA isolation; and (iii) Lysis buffer; were tested with modifications. All three methods did …
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Optimizing Conditions for Cloning cDNA from Chicken Brain
… of specific steps. These steps included RNA isolation, competent cell preparation, and cell transformation. The study results suggest RNA isolations can be increased with an augmented ethanol precipitation and include a shorter protocol for competent cell preparation which increases …
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Protective effect of TM-25659, known as TAZ activator, on high-fat diet-induced insulin resistance in C57BL/6J mice
… 2. Cell culture 10 3. Immunoblot analysis 11 4. RNA isolation and quantitative real-time polymerase chain reaction (PCR) 12 5. siRNAs 13 6. Uptake of 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino-2-deoxyglucose (2-NBDG) 14 7. Animal experiments 14 8. FGF21 secretion 15 9. IL-1β assay 15 10. …
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Differential expression of small RNAs in soybean
Small RNAs are non-protein coding RNAs that regulate gene expression in plants primarily by cleaving mRNA. Two major classes of small non-coding RNAs are microRNAs (miRNAs) and short interfering RNAs (siRNAs) of 21-24 nucleotides. I selected some small RNAs that show tissue specificity based on …
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Using RNA sequencing to identify transcriptomic differences in the renal pelvis
… in this thesis we optimized cell and tissue RNA isolation methods to obtain higher yields and high-quality RNA for our studies. We use a combination of transcriptomic approaches including bulk and single-cell RNA sequencing to reveal gene expression in multiple renal pelvis cell types, …
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Molecular Characterization of the Heat Shock-Regulatedgroesl Operon in Lactobacillus Zeae Strain 102S
… shock response genes groESL (HSP60/10). A rapid RNA isolation procedure was developed, and specific heat induction conditions for the groESL operon were determined by Northern and slot blot analyses. Changes in the level of the molecular chaperone GroEL (HSP 60) were analyzed by Western blotting …
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Regulation von Faktoren der Angiogenese durch die kurzkettige Fettsäure Butyrat
… stützten sich einerseits auf die Analyse der mRNA und der Proteine. Hierfür wurden die Zellkultur, die RNA-Isolation, ein RNase Protection Essay (RPA), der Westernblot und ein Enzyme-Linked Immunosorbent Assay (ELISA) durchgeführt. Die Analysen zeigten einen positiven Effekt auf die …
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Evaluation of small extracellular vesicular microRNAs as biomarkersfor exposure of nicotine and E-liquids on human lung cells
… health effects. In this study, we explored miRNAs from small extracellular vesicles (sEVs) for nicotine and E-liquid-associated biomarkers. We investigated miRNAs using NGS and further focused on a large miRNA cluster on C14q32 (C14MC), known to be dysregulated in lung-associated diseases. We …
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High Throughput Sequencing of SARS-CoV-2
… of the process including initial sample quality, RNA isolation method, and sequencing platforms which all contribute to quality sequencing results. We experimentally determined that the Maxwell automated RNA extraction method was the preferred method for vRNA isolation. We experimentally …
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Development and Evaluation of a Multiplex Suspension Array Protocol for the Detection of Enteric Pathogens from Clinical Specimens
… when extracted with the Ambion® MagMAX™-96 Viral RNA Isolation Kit combined with the KingFisher® Magnetic Particle Processor.
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Mapping the Major Epitope(s) of the Glycoprotein of Ebolavirus with QB Phage Display System
… of 1012 pfu/ml, followed by precipitation, and RNA isolation. The recombinant RNA was used to obtain cDNA for PCR amplification. The amplified cDNA was analyzed by agarose gel electrophoresis and sequenced to confirm the presence, position, and orientation of the glycoprotein segment within the …
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Equine Trophectoderm Cells and Their Role in Fetal-Maternal Recognition
… the embryo to the mare, a process known as maternal recognition. Six days after fertilization, the trophectoderm (TE), a placenta precursor is formed. Signals emanating from the TE to the uterine environment are critical to maternal recognition of pregnancy. The identity of factors necessary for …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… PCR (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA isolation, cDNA synthesis and real time PCR amplification have been optimized. Oligonucleotide primers were designed to amplify 165 bp specific to MAP and the …
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Molecular Basis and Modification of a Neural Crest Deficit in a Down Syndrome Mouse Model
… the established deficits. Through the use of RNA isolation techniques and cell culture systems of cell from the PA1 and NT of E9.5 Ts65Dn, Ts1Rhr, and control embryos, we established that trisomic genes Dyrk1a and Rcan1 ara dysregulated in both structures and that these two genes may interact. …
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Effect of Corn Quality and Enzyme Supplementation on Broiler Performance, Gastrointestinal Enzyme Activity, Nutrient Retention, Intestinal Mucin, and Jejunal Gene Expression
… tissue on d 28 was also collected for total RNA isolation and a subsequent genome-wide microarray assay. On d 50, 54 birds per trt were processed to evaluate carcass yield. Interactions of ME and AZ were observed in both EXP 1 and 2. In EXP 1, interactions of ME and AZ on BWG were observed …
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Smad Proteins and theTgf-Beta Signaling Pathway During In Vitro Progression of Hpv16-Immortalized Human Keratinocytes' and 'Microarray Gene Expression Profiling of Exfoliated Cervical Cells'
… We performed microarray experiments on RNA samples isolated from exfoliated cervical cells to search for biomarkers that could predict persistent HPV16 infections, the ones at higher risk of progressing into cervical cancer, which would constitute a cheaper, non-invasive and more …
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RNA helicase MOV10 is essential for gastrulation and normal CNS development
… we demonstrated that FMRP associates with the RNA helicase Moloney Leukemia Virus 10 (Mov10) in brain and modulates its translational activity through the microRNA (miRNA) pathway. FMRP is critical for normal cognition and our findings hypothesize a role for Mov10 in brain function. …
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Molecular Mechanisms of Antibacterial Resistance Among Pseudomonas aeruginosa: Multi-Drug Efflux Pumps and Their Interactions with Other Resistance Mechanisms
… pumps had varying results depending upon which RNA isolation method and endogenous controls w'ere utilized. When RNA was isolated by the hot phenol method and analyzed by semi-quantitative RT-PCR, similar conclusions were reached with other investigators as data demonstrated a growth-phase …
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