Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 135 for “"RNA interference(RNAi)"”.
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Das Sap47-Gen aus Drosophila melanogaster : Gezielte Mutagenisierung und Suche nach Interaktionspartnern
… Mutagenese durch homologe Rekombination, RNA interference (RNAi) und Transposon Remobilisierung. Um einen Interaktionspartner für das SAP47 Protein zu identifizieren wurden ein Yeast-Two-Hybrid System und das "CytoTrap" Verfahren eingesetzt.
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Development of a SCA7 patient-derived lymphoblast cell model for testing RNAi knock-down of the disease-causing gene
… been exploited in a potential allele-specific RNA interference (RNAi) based therapy, in order to knock down the expression of the mutant transcript in heterozygous patients. Although this approach has been tested in an artificial cellbased model of SCA7, focus has shifted towards testing the …
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Engineering periodic short hairpin RNA delivery systems for enhanced therapeutic efficacy
RNA interference (RNAi) presents a highly promising approach for cancer therapeutics via specific silencing of disease-implicated genes, but its clinical translation remains severely limited by barriers in delivering short interfering RNA (siRNA). Numerous delivery vehicles have been developed to …
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teashirt is required for head-versus-tail regeneration polarity in planarians
… Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes [beta]-catenin-1, wntl, Dvl-1/2, or wntless. teashirt was required for [beta]-catenin-I-dependent …
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RNAi based allele-specific silencing of the disease-causing gene in black South African patients with SCA7
… linked to a common ancestor. The manipulation of RNA interference (RNAi) has been used with increasing success to selectively knockdown the expression of disease-causing genes at the RNA level. Thus, the possibility of applying this method to SCA7 in South Africa was considered. However, the …
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Characterization of the roles of Xrn1p in small-RNA-mediated gene-silencing pathways
RNA interference (RNAi) is a small-RNA-mediated gene-silencing pathway that is involved in viral defense, transposon silencing, heterochromatin formation, and post-transcriptional gene silencing. Most RNAi pathways are initiated by long, dsRNA which is processed by Dicer into small interfering RNAs …
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The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives
RNA Interference (RNAi) is a natural cellular process that silences the expression of a target gene in a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is developing an effective delivery system for siRNAs. Recently, fatty acid …
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Functional genomic studies of regeneration in the planarian Schmidtea mediterranea
… gene. Functional analysis using double-stranded RNA-mediated RNA interference (RNAi) was implemented to determine effects on regeneration. The hypothesis was that effectors of regeneration are only conserved in animals capable of somatic tissue regeneration as adults. No genes fitting that …
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Elucidating the role of effector caspases in immune development using lentiviral RNAi
… interaction between them is important. We used RNA interference (RNAi) to examine their roles in immune cells. RNAi has revolutionized the field of mammalian genetics by expediting the interrogation of gene function. Endogenous genes are targeted for silencing by the introduction of double …
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Drosophila TRPA1 controls thermotactic behavior
… were unknown. We used a reverse genetic RNA interference (RNAi)-based strategy to identify Drosophila TRPA1 as a key regulator of thermotaxis. Larvae in which dTRPA1 expression has been knocked down with RNAi fail to avoid regions of moderately elevated temperature (~310C--350C).
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation
… critical for mosquito reproduction. Utilizing RNA interference (RNAi), the research identifies DP-1 as a significant regulator of follicle growth post-eclosion (PE), highlighting its vital role in the mosquito reproductive regulatory pathway. The experimental approach included RNAi-mediated …
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RNAi-related genes in Toxoplasma gondii.
RNA interference (RNAi) is an evolutionarily conserved mechanism through which double-stranded RNA (dsRNA) triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced …
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Computational prediction of RNA-based gene regulatory mechanisms in human and Tetrahymena
… impact of gene regulation mediated by small RNAs (sRNAs) is just beginning to come into focus. RNA interference (RNAi) pathways have been shown to mediate processes such as genomic rearrangement in ciliates and developmental timing and tissue differentiation in plants and animals. Here we …
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The Role of Dax-1 in Regulating Pluripotency in mouse Embryonic Stem Cells
… not as well understood. Recent studies employing RNA interference (RNAi) to specifically reduce the expression of the Dax-1 gene in mouse ES cells found that upon the knock down of Dax-1, ES cells differentiated. These findings indicate that Dax-1 functions in a novel role in the maintenance of a …
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Loss of function of Gene X protects against α-dicarbonyl stress through the skn-1 pathway in C. elegans
… an unbiased screen for the human SNPs utilizing RNA interference (RNAi) of the <em>C. elegans </em>orthologs, and discovered that RNAi of <em>Gene X </em>ameliorated α-dc pathologies such as neuropathy and stress. To understand the role this gene plays in regulating these pathologies, we examined …
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Translational regulation by short RNAs in mammalian cells
… are transcribed in the nucleus into messenger RNA, spliced, and exported to the cytoplasm, where they are translated into proteins. It was therefore a profound surprise when RNA itself, in the form of [approx.]21nt short RNAs, was discovered to have major generalized roles in the regulation of …
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MicroRNAs in cellular transformation and tumorigenesis
MicroRNAs (miRNAs) are a novel class of small (approximately 23 nucleotides long), highly conserved, non-coding RNAs that function by broadly regulating gene expression. In animals, this regulation is achieved via interaction with target messenger RNAs (mRNAs), largely through their imperfect base …
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Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort
… disease particularly amenable to allele-specific RNA interference (RNAi)-based therapy. Thus, this study also evaluated the feasibility of these cell models as a vehicle to test previously-developed RNAi therapeutics, using the alteration of expression of key transcripts as a phenotypic marker. …
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Liver fluke development: a target for parasite control?
… them and examine downstream effects. Using an RNA interference (RNAi) platform, the function of a key effector (fgfrA) was investigated and silenced juveniles exhibited a significant reduction in the number of EdU+ cells. The findings of this study highlight the stem-like characteristics of …
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