Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 334 for “"RNA interference"”.
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The Mechanism of RNA Interference in Neurospora
In the canonical RNA interference (RNAi) pathway, small-interfering RNA (siRNA) duplexes generated by Dicer are incorporated into the RNA-induced-silencing complex (RISC), and subsequently converted to single-stranded siRNA. Generation of single stranded siRNA is a pre-requisite for recognition and …
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The Role of TNRC6 in RNA Interference
Small RNAs can influence translation, splicing, transcriptional activation, and transcriptional repression, through the RNA interference (RNAi) pathway. TNRC6, also known as GW182, and Argonaute (AGO) are the core proteins of RNAi. TNRC6 is a scaffolding protein that associates with AGO and bridges …
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Treatment of Ischemia Reperfusion Injury with RNA Interference
… with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% (p=0.968). Combinations …
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Engineering a Photo-Control Mechanism for RNA Interference
RNA interference (RNAi) is a phenomenon in which RNA molecules elicit potent and sequence-specific post-transcriptional gene silencing. Nucleic acid chemical modifications have been incorporated to improve their pharmacological properties. Despite numerous developments in chemical modifications for …
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Tackling Computational Challenges in High-Throughput RNA Interference Screening
Since the discovery of RNAi decades ago, it has been increasingly used in biomedical and biological research. The success of analyzing single genes using siRNAs has resulted in the large-scale application of RNAi for genome-wide loss-of-function phenotype screening while reducing cost and …
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The Function and Mechanism of RNA Interference in Neurospora
RNA interference (RNAi) is a conserved gene silencing mechanism important for various biological processes, including developmental timing, genome defense, and heterochromatin formation. RNAi is triggered by double stranded RNA (dsRNA), which is processed by Dicer to siRNA. siRNA is loaded onto …
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Identifying Novel Contributors to RNA Interference in Aedes aegypti
… dengue and chikungunya. The small interfering RNA (siRNA) pathway is a critical immune response for controlling viral replication in Aedes aegypti. The goal of this research is to identify components of the Aedes aegypti genome that influence this pathway. A transgenic mosquito strain that …
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Localization and Function Of RNA Interference in the Cerebral Cortex
… gene expression by harnessing the machinery of RNA interference (RNAi) pathway via small hairpin RNAs (shRNA) delivered by viral vectors. Using this technology we sought to influence plasticity of the mouse vibrissal barrel cortex. In this model system, chronic whisker plucking reliably leads to …
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RNA-interference e Farmacogenomica: dall'analisi dell'RNA agli effetti dei farmaci
… sulle rappresentazioni dei fenotipi, microRNA, grug, proteine, effetti collraterali e fattori di trascrizione. Tale ricchezza di informazioni è una valida risorsa per i ricercatori biomedici.
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Discovery and biochemical characterization of RNA interference in budding yeast
RNA interference (RNAi) is a eukaryotic pathway for the post-transcriptional regulation of gene expression. In the simplest form of RNAi, a double-stranded RNA (dsRNA) trigger is converted into small-RNA duplexes by the Dicer enzyme. These duplexes are then loaded into the effector protein …
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RNA interference targeting Glucose-Regulated-Protein 78 induces HepG2 cell Apoptosis
… targeting GRP78 with short-interfering RNA (siRNA), potent GRP78 silencing is anticipated by the RNA interference (RNAi) pathway. Silencing GRP78 expression is expected to compromise cell signaling activity, ultimately resulting in cancer cell death. Towards this effect, I have shown …
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Sustained hydrogel-based delivery of RNA interference nanocomplexes for gene knockdown
Scaffold based delivery of RNA interference (RNAi) molecules such as free small interfering RNA (siRNA) and microRNA has recently begun to be employed towards treatment of diseases such as cancer, bone regeneration, muscular dystrophy and cardiovascular disease. Effective translation from bench …
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Genetic factors affecting the RNA interference pathway of Aedes aegypti mosquitoes
… cause tremendous social and economic impact. RNA interference (RNAi) plays a crucial role in the vector competence of mosquitoes and is often targeted in studies involving mosquito innate immunity, genetics-based vector control strategies, and the development of viral-resistant transgenic …
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TOWARDS AN IN VITRO MODEL FOR RNA INTERFERENCE- MEDIATED SUPPRESSION OF S100A8
… was to assess the efficacy of short-hairpin RNAs (shRNAs) in suppressing murine S100A8 expression in vitro as a preliminary model to the study of S100A8 knockdown in CF mice. Stimulation of murine macrophage-like P388D1 and granulocytic 32D cells failed to induce S100A8 expression. COS-7 …
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Silencing mutant Huntingtin by RNA interference for the treatment of Huntington Disease
… testing to develop potential treatments for HD. RNA interference (RNAi) therapy is a targeted gene silencing approach whereby synthetic RNA constructs are shuttled into the cell by viral vectors and used by the cell’s endogenous RNAi machinery to silence a gene of interest. RNAi therapy holds …
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RNA Interference and cyclooxygenase-2 (COX-2) regulation in colon cancer cells
… attention has recently shifted onto the RNA interference (RNAi) phenomenon. Gene silencing mediated by RNAi machinery is based on short RNA molecules, small interfering RNAs (siRNAs) and microRNAs (miRNAs), that are fully o partially homologous to the mRNA of the genes being silenced, …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… first successful Phase III clinical trial of a RNA interference (RNAi)-based therapeutic is a major achievement in the field. Synthetic RNAi therapeutic oligonucleotides are either first cleaved by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and …
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An RNA-interference-based platform for genome analysis and engineering in Saccharomyces cerevisiae
… to its simplicity and effectiveness, the pooled RNA interference (RNAi) screening should satisfy the requirement of directed genome evolution. The RNAi pathway is absent in Saccharomyces cerevisiae, also known as the baker’s yeast. Recently, functional RNAi machinery has been reconstituted in …
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RNA interference of worker sterility genes: Testing mechanisms of reproductive regulation in Apis mellifera
… not well understood. Here, this study used RNA interference to knock down the expression of single genes (fruitless and ftz-f1) that have been previously identified as influential in regulating this response to the queen’s pheromone. Cage experiments were performed to monitor changes to …
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