Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"RNA helicases"”.
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Role of DDX RNA helicases in cancer cells
Ester Cannizzaro ROLE OF DDX RNA HELICASES IN CANCER CELLS Abstract DEAD-BOX (DDX) RNA helicases are a large family of proteins characterized by the presence of a DEAD/H (Asp-Glu-Ala-Asp/His) motif. Their main function is to unwind double stranded RNA to promote downstream molecular events. They …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
Pre-mRNA (precursor messenger RNA) splicing is a fundamental process in eukaryotic gene expression. In order to catalyse the excision of the intervening intronic sequence between two exons, the spliceosome is assembled stepwise on the pre-mRNA substrate. This ribonucleoprotein machine is extremely …
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THE ROLE OF ADARS-INTERACTING RNA HELICASES IN SHAPING THE RNA EDITOME IN CANCER
Adenosine-to-inosine (A-to-I) RNA editing, primarily catalysed by the ADAR family of proteins, is oftentimes dysregulated in cancers. However, the regulatory mechanisms controlling editing frequency in cancer cells remain largely elusive. Besides factors affecting the expression and activity of …
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Elucidating the functions of RNA helicases DDX3X and DDX3Y in normal and malignant B cells
DDX3X is an RNA helicase involved in multiple stages of RNA biogenesis, the most well-studied being as a regulator for translation. Its Y chromosome paralogue, DDX3Y shares 92% amino acid conservation. DDX3X is ubiquitously expressed and considered pan essential. While DDX3Y is widely transcribed, …
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HAGE, a novel cancer/testis antigen with strong potential as a target for immunotherapy against cancers
… in several carcinomas and leukaemias at the mRNA level only. This study proposed to investigate HAGE as a potential target for immunotherapy in a murine tumour model. HAGE mRNA was found to be expressed in a small proportion of carcinomas, some melanomas and in a strong proportion of chronic …
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CHARACTERIZATION OF THE FUNCTION OF THE DEAD-BOX RNA HELICASE DBP2
… there are highly coupled mechanisms that require RNA-binding proteins to facilitate gene expression. Proper RNA structure and ribonucleoprotein (RNP) complex formation are critical for gene expression. DEAD-box proteins are the largest class of RNA helicases that play fundamental roles in RNA and …
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Interactions and Functions of the Ubiquitin Specific Protease 7 in Human Cells
… interactions with FBXO38 and two DEAD/DEAH-box RNA helicases, DDX24 and DHX40. I show that USP11, PPM1G, DDX24, TRIP12 and FBXO38 bind USP7 through its TRAF binding pocket, while DHX40 interacts through the Ubl2 pocket. Motifs in USP11 and DDX24 that are critical for USP7 binding were also …
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Multiple Levels of Regulation of Cyclin-dependent Kinase Inhibitor 1A (p21/WAF-1) by the <i>DDX5 </i>Gene
… an archetypical member of the DEAD box family of RNA helicases, exhibits both ATPase and RNA helicase dependent/independent activities. To date the p68 protein has been implicated in most parts of RNA metabolism including ribosome biogenesis, transcription, RNA decay, miRNA processing and pre-mRNA …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… as an intracellular viral double-stranded (ds)RNA sensor through molecular cloning and the characterization of a HCV replication permissive cell line defective in IRF-3 activation. The NS3/4A protease disrupted IRF-3 signaling through both the RIG-I pathway as well as the homologous MDA-5 …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… as an intracellular viral double-stranded (ds)RNA sensor through molecular cloning and the characterization of a HCV replication permissive cell line defective in IRF-3 activation. The NS3/4A protease disrupted IRF-3 signaling through both the RIG-I pathway as well as the homologous MDA-5 …
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Functional Investigation of RNA Viral Helicases as Molecular Targets for the Discovery of Novel Antiviral Agents
… potential future spillovers and for other (+)ssRNA viruses that still lack approved pharmacological treatments such as Flaviviruses. Small molecule development stands out as the most promising approach, as it enables possibly oral administration, stockpiling, combinatorial therapy. (+)ssRNA …
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Structural Studies of the Nucleolar Stages of Ribosome Biogenesis in Yeast
<p>The ribosome is the RNA-protein machine responsible for the essential task of translating mRNA into proteins. Ribosomes are heterodimers made up of a small subunit (SSU, 40S) and a large subunit (LSU, 60S). At the interface of these subunits, the mRNA is decoded by the small subunit and peptide …
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Mechanism of Ubiquitin Induced Activation of RIG-I Like Receptors in Antiviral Innate Immunity
… MDA5, are essential for the detection of viral RNA in the cytoplasm. Upon viral recognition, RIG-I and MDA5 activate the mitochondrial signaling protein AVS, and initiate downstream signaling pathways that activate the transcription factors NF-kappaB and IRF3. These transcription factors …
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Dissecting the role of RNA helicase DDX3X in mRNA translation and B-cell function during immune responses
… time points after B-cell activation on both the RNA and protein level to markers of mRNA translation in germinal centre B-cells. Furthermore, we have performed ribosome profiling studies to establish which mRNAs are differentially translated between WT and DDX3X-depleted B-cells. Ultimately, our …