Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"RNA granules"”.
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Cell-Free Formation of RNA Granules
Asymmetric RNA localization is a mechanism by which a cell can spatially and temporally regulate the translation of RNAs. This mechanism is essential for many developmental processes such as germ cell formation in Drosophila embryos, as well as establishment of cell polarity and synaptic plasticity …
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Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells
… novel interactions between SMN and two RNA-binding proteins (RBPs) known to be components of axonal RNA granules. More specifically, we demonstrated that SMN interacts with HuD and SERBP1 in a direct fashion in foci-like structures along neurites of motor neuron-derived cells. We have …
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Chemical Footprinting of Polymeric Structure of hnRNPA2 Low Complexity Domain
Many DNA and RNA regulatory proteins contain polypeptide domains that are unstructured when analyzed in cell lysates. These domains are typified by an over-representation of a limited number of amino acids and have been termed prion-like, intrinsically disordered or low complexity domains. These …
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Lysosomes and Ribonucleoprotein Dynamics in Neurons
… lysosomal surface. ANXA11 is also a component of RNA granules, and its bipartite domain organization allows it to act as a tether between RNA granules (N-terminal phase separation) and the lysosomal surface (C-terminal phospholipid binding). We find that ANXA11 enables RNA granule hitchhiking on …
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Molecular mechanisms underlying morphological effects of protein kinase C under normal conditions and cellular stress.
… intermediate filament peripherin and several mRNA-binding proteins. Peripherin and three RNA-binding proteins G3BP2, PABPC1, and IGF2BP3 were further investigated regarding their putative function in PKCε-mediated neurite outgrowth. However, no involvement could be detected for any of the …
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Axonal Translation and Links to Neuropathies
… of translation dynamics in response to external stimuli. Further studies have shown that local translation can occur on Rab7a-associated late endosomes, where mRNA recruitment and translation are coordinately regulated. Notably, mRNAs encoding mitochondria-related proteins are found …
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Investigating Microtubules Inside Cells with Cryo-Electron Tomography
… aimed to label dynein cargoes, with a focus on RNA granules, to target them by cryogenic correlative light and electron microscopy (cryo-CLEM). I describe targeting strategies and attempts at cryo-CLEM, most of which were unsuccessful. As an alternative, I established the sample preparation of …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… directly to Filamentous-actin. However, via alternative promoter usage and alternative 3’ end processing, the nesprins are able to generate multiple mRNA transcripts, leading to the production of diverse tissue specific isoforms with potential roles beyond the NE. To explore further the capacity …
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Regulating transcript fate through RNA elements in the 3' untranslated region
… on the post-transcriptional regulation of mRNAs via miRNAs, other small RNAs, and RNA binding proteins. Controlled translation of transcripts has been shown to play a vital role in rapid response to changing conditions such as dendritic tree formation, ischemia, heat shock, and immune …
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Mechanisms of dynein-based mRNA transport processes in human cells
… both space and time through the positioning of mRNA molecules in the cytoplasm. This process is commonly mediated by molecular motors, which transport mRNAs along the cytoskeleton to specific cellular regions. Despite the widespread occurrence of mRNA transport, the precise mechanisms by which …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… of finely tuning translation of selected mRNAs, do exist. Multiple types of ribosomes are defined by several elements, such as their composition, and their association with different proteins, known as ribosome-associated proteins (RAPs). In particular, these proteins can exert a direct …