Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"RNA genome"”.
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RNA genome annotation with a focus on T. brucei
… (UTRs) and UTR-indicating patterns in the genome of T. brucei. T. brucei is an interesting organism, and as the cause of African sleeping sickness -- which infects 300,000-500,000 people and a significant number of cattle annually -- is currently the subject of considerable research. Using …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
… systems that permit replication of the viral RNA genome and virus particle production. However, the exact functions of the viral proteins, particularly those engaged in RNA synthesis, are poorly understood. It is thought that NS4B, one of the replicase components, induces the formation of …
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Molecular characterization of novel soybean-associated viruses identified by high-throughput sequencing
High-throughput sequencing of mRNA from soybean leaf samples collected from North Dakota and Illinois soybean fields revealed the presence of two novel soybean-associated viruses. The first virus has a single-stranded positive-sense RNA genome consisting of 8,693 nt that contains two large open …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… catalytic antibody that binds to DNA and RNA then degrades them without sequence specificity as well known as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it …
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Biochemical and Genetic Analyses of Rna-Protein Interactions
… of replicase and functions to encapsidate the RNA genome of the phage. The coat protein accomplishes these functions by interacting with a 21 nucleotide stem-loop structure located within the translation initiation region of the bacteriophage replicase gene. A great deal is known about the …
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Determinants of Rotavirus Polymerase Localization and Activity
… during infection where it replicates the virus' RNA genome. The work described in this dissertation focused on identifying region(s) of VP1 essential for its viroplasmic localization and its function as a polymerase. We found that a single amino acid change in a region of the polymerase called …
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VIRUS-HOST INTERACTIONS AND THE IMPACT ON HOST CELLULAR MECHANISMS
Paramyxoviridae constitutes an RNA-genome virus family that includes the emergent and deadly Nipah (NiV) and Hendra (HeV) viruses. These viruses possess substantial pandemic potential and exhibit an expansive host range, a characteristic underpinned by their conserved ephrin receptors. Being a …
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Tombusvirids Avoid and Exploit a Plant Exoribonuclease
Tombusviridae is a family of plus-strand RNA plant viruses that contain single-stranded RNA genomes with no 5' cap or 3' poly(A) tail. The 5' cap is an essential post-transcriptional modification that increases the stability of mRNA molecules, by protecting them from 5'-to-3' exoribonuclease decay. …
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Statistical thermodynamics of virus assembly
… of HIV-1 capsids. Secondly, the problem of RNA genome packaging inside spherical viruses is studied using RNA condensation theory. For weak adsorption strength of capsid protein, most RNA genomes are located at the center of the capsid. For strong adsorption strength, RNA genomes peak near …
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Countermeasures Against Viral Hemorrhagic Fevers
… often fatal outcomes. VHFs can cause both internal and external bleeding. The virus families include: Filoviridae, Arenaviridae, Bunyaviridae, and Flaviviridae. All these viruses are enveloped and contain a single-stranded RNA genome. However, the viruses differ in their pathogenesis, …
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Molecular Mechanisms Behind Sars-Cov-2 Induced Host Genome Restructuring
… least in part, to significant alterations in 3D genome organization and epigenetic landscape following SARS-CoV-2 infection that are distinct from and more severe than changes caused by other viral infections or immune stimulants. While these changes at the chromatin level are important for …
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Grapevine Fanleaf Virus: Biology, Biotechnology And Resistance
… most grape growing regions and has a bipartite RNA genome. The three goals of this research were to (1) advance our understanding of GFLV biology through studies on its satellite RNA, (2) engineer GFLV into a viral vector for grapevine functional genomics, and (3) discover a source of resistance …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… coat protein and produced genomic and subgenomic RNAs in ratios similar to those observed in protoplasts inoculated with viral RNA. Genomic RNA regions were identified that either down-regulated promoters located 5'-proximally on positive strand RNA strands or altered the site of transcription …
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Biochemical and Structural Characterization of Divergent Members of the Protein Kinase Family
… Glutamylation, and NiRAN-catalyzed RNA capping. A Legionella pneumophila effector SidJ adopts a kinase fold yet catalyzes calmodulin (CaM)-dependent glutamylation to inactivate the SidE ubiquitin ligases. SidJ becomes activated upon translocation into the host cell and uses cellular …
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Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… a trisegmented, single-stranded, negative-sense RNA genome, consisting of L, M and S segments. The virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA …
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MicroRNA-122 and Poly(C)-Binding Protein 2 Bind to the Hepatitis C Virus Genome, Regulating Viral Replication and Genome Structure
Hepatitis C virus (HCV) has a positive-sense RNA genome which is used as the template for both viral translation and negative-strand RNA synthesis. As both processes work in opposite directions on the genome the virus requires a mechanism to coordinate what process is acting on a given copy of …
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HIV needs the small molecule IP6 to build its capsid
… a mature capsid. The capsid protects the viral RNA genome which is reverse-transcribed into DNA, trafficked to the nucleus, and integrated into the host genome. Both immature and mature HIV capsid lattices need to maintain a delicate balance of stability to allow efficient assembly without …
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Upregulation of Reactive Oxygen Species During The Retrovirus Life Cycle and Their Roles In A Mutant of Moloney Murine Leukemia Virus, Ts1-Mediated Neurodegeneration
… infection, retroviral DNA is generated from its RNA genome and integrated into the host genome. In this study, we identified the life cycle of <em>ts</em>1 in the infected astrocytes. During the infection, we observed reactive oxygen species (ROS) upregulations: one at low levels during the early …
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A Proteomic Approach to Elucidating the Function of Picornavirus 2A Protease
… metabolism, protein synthesis, nuclear and RNA transport across the nuclear pore complex, apoptosis, and innate immune responses. Picornaviruses are the most dominant human disease-causing viruses and present an excellent clinical target for research studies into their molecular mechanisms. …
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Studies of Entry, Reverse Transcription, and Regulation of Splicing in Retroviruses
… when the discovery that retroviruses copy their RNA genome into DNA was made. The same enzyme that performs this step, reverse transcriptase (RT), also revolutionized molecular biology when it was used as a tool to generate full length cDNA clones of expressed genes. The impact of retroviruses on …
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