Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"RNA elements"”.
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Regulating transcript fate through RNA elements in the 3' untranslated region
… on the post-transcriptional regulation of mRNAs via miRNAs, other small RNAs, and RNA binding proteins. Controlled translation of transcripts has been shown to play a vital role in rapid response to changing conditions such as dendritic tree formation, ischemia, heat shock, and immune …
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Discovery and Validation of New Regulatory RNA Elements in Chlamydia trachomatis
… of a newly defined family of non-coding RNAs (ncRNAs) that are differentially expressed during the developmental cycle and the induction of persistence and reactivation (Using IFNγ and Carbenicillin). Non-coding RNAs were initially identified using an intergenic tiling microarray and were …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
… regulate gene expression. Specifically, various RNA-mediated genetic control elements have been discovered in the past decade through a combination of genetics, bioinformatics, and transcriptomic approaches. Together, these RNA elements control the expression of many genes involved in diverse …
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Regulation of Human Papillomavirus Type 16 Late L1 mRNA Splicing
… progression to cervical cancer. HPV-16 uses alternative splicing to regulate expression of early and late genes. HPV RNA elements and cellular factors control the expression level of viral proteins by regulating alternative splicing. This project was carried out to enhance our understanding of …
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Regulation of hnRNP A1 Cellular Localization by Protein Kinases and its Biological Impact
Human Rhinoviruses (HRVs) utilize Internal Ribosome Entry Sites (IRES) to drive viral protein synthesis. IRESs are specialized RNA elements present within the 5’ UTR of mRNAs that recruit ribosomes independently of the 5’ m7G cap structure. hnRNP A1 (heterogeneous nuclear ribonucleoprotein A1), a …
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Non-Coding RNA Features Critical to the Replication of HIV-1
<p>The HIV-1 genome contains RNA sequences and structures that control many aspects of viral replication including, but not limited to transcription, splicing, nuclear export, translation, packaging and reverse transcription. Despite this extensive existing catalogue of RNA sequences that are …
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Selenoprotein-Biosynthese in Archaea
… Folgende Ergebnisse wurden erhalten: a) Die tRNASec von M. jannaschii konnte mit gereinigter Seryl-tRNA-Synthetase von E. coli korrekt in vitro mit L-Serin beladen werden. Die Umwandlung der Seryl-Gruppe in eine Selenocysteyl-Gruppe war mit gereinigter Selenocystein-Synthase und …
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Investigating and Reprogramming RNA Folding with Molecular Probes
Ribonucleic acid (RNA) performs versatile, essential functions in all known organisms and viruses. These functions require the RNA to fold into specific secondary and tertiary structures, and in many cases to switch among multiple structural states. Predicting and experimentally determining RNA …
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Architecture of eukaryotic mRNA 3' end processing machinery and insights into the mechanism of polyadenylation
Almost all eukaryotic messenger RNAs (mRNAs) have a polyadenosine (polyA) tail at their 3′ end that is added by a multi-protein complex known as cleavage and polyadenylation factor (CPF). CPF, along with accessory cleavage factors (CF) IA and IB, cleaves the pre-mRNA within the 3' untranslated …
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Sequence determinants of pri-miRNA processing
MicroRNAs (miRNAs) are short RNAs that regulate many processes in physiology and pathology by guiding the repression of target messenger RNAs. For classification purposes, miRNAs are defined as ~22 nt RNAs that are produced by the cleavage of endogenously transcribed hairpins. From a cellular …
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Structural RNA Homology Search and Alignment Using Covariance Models
Functional RNA elements do not encode proteins, but rather function directly as RNAs. Many different types of RNAs play important roles in a wide range of cellular processes, including protein synthesis, gene regulation, protein transport, splicing, and more. Because important sequence and …
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Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… a trisegmented, single-stranded, negative-sense RNA genome, consisting of L, M and S segments. The virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA …
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Biochemical and functional characterization of human RNA binding proteins
RNA not only shuttles information between DNA and proteins but also carries out many other essential cellular functions. Nearly all steps of an RNA's life cycle are controlled by approximately one thousand RNA binding proteins (RBPs) that direct RNA splicing, cleavage and polyadenylation, …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
Base pairing small regulatory RNAs (sRNAs) are important post-transcriptional regulators of gene expression in bacteria. These sRNAs deploy novel mechanisms to regulate mRNA targets leading to various physiological outcomes during stress conditions including, but not limited to, iron starvation, …
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Making Ribosomes: Biochemical and Structural Studies of Early Ribosome Biogenesis in Yeast
… the cell. In yeast, it is made of four ribosomal RNAs and 79 proteins, asymmetrically divided in a small and large subunit. In a growing yeast cell, more than 2000 ribosomes are assembled every minute. The ribosome is assembled through a highly complex process involving more than 200 trans-acting …
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Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species
… key steps in eukaryotic gene expression is pre-mRNA splicing, whereby intronic sequences are excised from immature pre-mRNA transcripts and the remaining exonic sequences are joined together. This process is catalyzed by the spliceosome, a large complex of proteins and RNAs. A variety of RNA …