Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"RNA degradation"”.
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Estrogen Regulates Vitellogenin Gene Transcription and Messenger-Rna Degradation in Xenopus Laevis Liver
Vitellogenin gene transcription and mRNA degradation rates were measured in Xenopus laevis liver using pulse labeling and pulse chase techniques. Estrogen regulates the accumulation of vitellogenin mRNA through three processes: (1) There is a selective transcriptional activation of the vitellogenin …
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Identifying Subunit Organization and Function of The Nuclear Rna Exosome Machinery
… SUBUNIT ORGANIZATION AND FUNCTION OF THE NUCLEAR RNA EXOSOME MACHINERY </strong></p> <p><strong>Jillian Strother Losh, A.S., B.S. </strong></p> <p>The eukaryotic RNA exosome processes and degrades many classes of RNA. It is present in the nucleus and the cytoplasm, highly evolutionarily conserved, …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… catalytic antibody that binds to DNA and RNA then degrades them without sequence specificity as well known as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it …
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A Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)
… gel electrophoresis revealed extensive ribosomal RNA degradation. Poly-Ã -hydroxybutyrate, abundant in the vegetative rods, was not detectable in the coccoid cells. The results suggest that the coccoid form of A. fasciculus is a degenerative form rather than part of a life cycle.
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Analyzing the functions of human polynucleotide phosphorylase (hPNPaseold-35)
RNA degradation plays a fundamental role in maintaining cellular homeostasis, along with being a part of normal regulatory mechanisms, whether it occurs as a surveillance mechanism eliminating aberrant mRNAs or during RNA processing to generate mature transcripts. 3’-5’ exoribonucleases are …
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DECIPHERING AND EXPLOITING ANEUPLOIDY IN CANCER
… of six isogenic clones via whole-exome and RNA sequencing, along with extensive CRISPR/Cas9 and drug screening, I explored their cellular dependency landscapes. In line with earlier findings, I observed increased DNA damage and p53 pathway activation in aneuploid cells. These cells also …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… necessitates complex molecular responses to external stimuli, including environmental change. As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… necessitates complex molecular responses to external stimuli, including environmental change. As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein …
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Small molecule-degrader conjugates for MALAT1 triplex degradation
… overexpression of a specific, long non-coding RNA sequence called MALAT1. Due to its uncommon triple helix structure, this transcript has a noteworthy stability, with a half-life of up to 15 hours, resulting in its build-up in the cell environment. This work details my efforts in the design and …
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Analysis of RNA stability in forensic specimens
Ribonucleic acid (RNA) analysis presents a unique, novel opportunity to answer a wide range of questions in forensic science. The dynamicity of the transcriptome has led to it being suggested as a novel source of diagnostic information in forensic pathology. However, RNA is exceptionally labile. …
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The Role of Endogenous Arabidopsis Proteins In Rna Silencing and Hc-Pro-Mediated Suppression of Silencing
<p> RNA silencing is a sequence-specific RNA degradation pathway that acts as an antiviral defense pathway. Viruses have evolved suppressors of RNA silencing, including the potyviral protein Helper Component-Proteinase (HC-Pro). Our focus on evaluating the mechanism of HC-Pro function has led us to …
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Stability And Recovery Of Rna In Biological Stains
In theory, RNA expression patterns, including the presence and relative abundance of particular RNA species, provide cell and tissue specific information that could be of use to forensic scientists. An mRNA based approach could allow the facile identification of the tissue components present in a …
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Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… in the human chromosome 3p21.3 region. TUSC2 mRNA transcripts could be detected on Northern blots in both normal lung and some lung cancer cell lines, but no endogenous TUSC2 protein could be detected in a majority of lung cancer cell lines. Mechanisms regulating TUSC2 protein expression and …
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Harnessing transcriptomic data to better understand gene regulatory mechanisms of neurodevelopmental disorders
… This thesis investigates the nonsense-mediated mRNA decay (NMD) pathway —a key post-transcriptional mechanism with critical implications for neurodevelopment and NDDs —using transcriptomic data.. To assess the role of NMD, I first create a novel framework which utilises a data-driven approach to …
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Unifying the features that determine substrate preferences of nanoRNases
RNA polymers are synthesized, degraded, and modified through several varied cellular processes. The ability of cells to degrade and recycle their RNA products is a ubiquitous and essential function. Specific subsets of endo- and exoribonucleases catalyze the reactions that ultimately replenish the …
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Angiotensin II-mediated Regulation of the Human Angiotensin II Type 1 Receptor Gene
… of cell-surface receptor and also its mRNA. We have investigated whether the 3'- and 5'-untranslated regions of the human AT1R mRNA mediate the degradation of hAT1R mRNA by post-transcriptional mechanisms in human adrenocortical carcinoma cell line (H295R cells). Protein kinase C (PKC) …
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The Sequence Landscape of Bacterial Genes is Shaped by Long-Range mRNA Folding
… of genes in regulatory networks has led to discernable sequence patterns in bacterial genomes observed in nature. Such patterns result from gene regulatory strategies that leverage sequence-dependent interactions with key cellular machineries and regulatory molecules. While numerous regulatory …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
Post-transcriptional RNA modifications, such as N6-methyladenosine (m6A), 2'-O-methylation (Nm), and 5-methylcytosine (m5C), are critical regulators of gene expression. Mapping these modifications at single-nucleotide resolution, however, remains a major challenge as current methods often suffer …
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Accessory Nucleases Provide Robust Antiparasite Immunity for Type III CRISPR-Cas Systems
… of immunological memory. Upon reinfection, short RNAs transcribed from the CRISPR locus can guide Cas proteins to the viral genome through complementary base pairing. Cas nucleases then destroy the invader's genome. To date, six major types and multiple subtypes of CRISPR systems exist, each with …
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