Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"RNA decay"”.
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The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay
Control of nuclear RNA stability is an important determinant of gene expression, but the factors involved in nuclear RNA decay remain largely unknown in higher eukaryotes. Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human …
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Assessing and understanding the generation and function of RNA decay intermediates in non-insect borne flaviviruses
… correctly to stimuli or to maintain homeostasis. RNA viruses must act to preferentially drive production of their own messenger RNAs (mRNAs) and proteins in order to successfully replicate and ensure continued infection. Due to the necessity for RNA viruses to remain in the cytoplasm, regulatory …
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Defining the Precision and Sequence Determinants of Protein Synthesis Rates
… I turn my focus towards understanding how RNA decay and processing rates, two poorly understood processes central to the quantitative tuning of protein synthesis, are encoded in bacterial mRNAs. Using high-resolution RNA end-mapping techniques in combination with genetic perturbations to …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… necessitates complex molecular responses to external stimuli, including environmental change. As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein …
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From cradle to grave: The influence of mRNA metabolism on plant stress responses
… necessitates complex molecular responses to external stimuli, including environmental change. As the intermediary between DNA and protein, mRNA is a crucial cog in plant gene expression, and consequently a key regulatory target. During stress, control of mRNA metabolism (and downstream protein …
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Relationship Between TDP-43 Toxicity and Aggregation in Saccharomyces Cerevisiae
… a natively dimeric protein and that binding to RNA inhibits aggregation. Corresponding genetic results in yeast in which specific components of the RNA-decay machinery have been knocked-out, indicate that the buildup of specific cellular RNAs is capable of counteracting TDP-43 aggregation and …
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The balance of poly(U) polymerase activity ensures germline identity, survival, and development in Caenorhabditis elegans
<p>Uridylation of RNAs has been found to be highly prevalent and conserved among eukaryotes. The untemplated addition of uridines to the 3' end of RNA molecules is catalyzed by poly (U) polymerase (PUP). Uridylation has a fundamental role in RNA decay. The emerging discoveries regarding uridylation …
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Alternative Mrna Splicing Redefines The Landscape Of Commonly Dysregulated Genes Across The Acute Myeloid Leukemia Patient Population.
… less than 10% of patients, suggesting that alternative mechanisms of gene disruption contribute to this disease. Here, I investigate pre-mRNA splicing events with significant variation and striking coregulation across distinct AML cohorts. I find that most splicing events are expected to alter …
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Analysis of RNA stability in forensic specimens
Ribonucleic acid (RNA) analysis presents a unique, novel opportunity to answer a wide range of questions in forensic science. The dynamicity of the transcriptome has led to it being suggested as a novel source of diagnostic information in forensic pathology. However, RNA is exceptionally labile. …
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Multiple Levels of Regulation of Cyclin-dependent Kinase Inhibitor 1A (p21/WAF-1) by the <i>DDX5 </i>Gene
… an archetypical member of the DEAD box family of RNA helicases, exhibits both ATPase and RNA helicase dependent/independent activities. To date the p68 protein has been implicated in most parts of RNA metabolism including ribosome biogenesis, transcription, RNA decay, miRNA processing and pre-mRNA …
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Destabilization of IL-8 mRNA by Anthrax Lethal Toxin: Demonstration of the Requirement for TTP and Examination of its Cellular Interactions
Control of mRNA stability is an important aspect in the regulation of gene expression. A well studied signal for rapid transcript decay in mammalian cells is the AU-rich element (ARE), which is found in the 3’ untranslated region (UTR) of many labile transcripts. These sequence elements confer …
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Analyzing the functions of human polynucleotide phosphorylase (hPNPaseold-35)
RNA degradation plays a fundamental role in maintaining cellular homeostasis, along with being a part of normal regulatory mechanisms, whether it occurs as a surveillance mechanism eliminating aberrant mRNAs or during RNA processing to generate mature transcripts. 3’-5’ exoribonucleases are …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
… regulate gene expression. Specifically, various RNA-mediated genetic control elements have been discovered in the past decade through a combination of genetics, bioinformatics, and transcriptomic approaches. Together, these RNA elements control the expression of many genes involved in diverse …
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THE ROLE OF THE RNA-BINDING PROTEIN TRISTETRAPROLIN IN CERVICAL AND COLORECTAL CANCERS
<p> Messenger RNA decay is a critical mechanism to control the expression of many inflammation- and cancer-associated genes. These transcripts are targeted for rapid degradation through AU-rich element (ARE) motifs present in the mRNA 3' untranslated region (3'UTR). Tristetraprolin (TTP) is an …
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Investigations of cellular stress-responsive proteins and PGF gene expression in human trophoblast
… in preeclampsia (PE), a leading cause of maternal morbidity and mortality. The pathophysiology of PE is thought to be manifested by a poorly perfused placenta hampered by hypoxic stress. Two stress-mediate angiogenic responses include post-transcriptional regulation of mRNA stability, and …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
… in these studies. The genomic and subgenomic RNAs of Sindbis virus strongly resemble cellular mRNAs as they are capped at their 5’ ends and polyadenylated at their 3’ termini. These features allow the viral RNAs to act like cellular mRNAs and make them prime substrates for the cellular mRNA …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
Almost all mature eukaryotic mRNAs contain a 3′ polyadenosine (poly(A)) tail, which promotes nuclear export of mRNAs, protects transcripts from exonucleolytic decay, and increases translation efficiency. As the poly(A) tail is involved in many steps of gene expression, its length is highly …
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Role of long non-coding RNAs in breast cancer progression
… of genome is transcribed into non-protein coding RNAs (ncRNAs), including thousands of long ncRNAs (lncRNAs). At present, we do not know the biological function of majority of these lncRNAs. MALAT1 (metastasis associated lung adenocarcinoma transcript1) is a lncRNA which its expression is …
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Regulation and function of the RNA-binding protein CELF1
… Coordinate regulation of the rate of decay of networks of functionally related mRNA provides a cell the ability to quickly and precisely alter its transcriptome in response to environmental signals. An important regulator of mRNA decay is the RNA binding protein CUGBP1 and Etr3 Like …
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The Regulation of ARE-Mediated mRNA Decay During Colorectal Tumorigenesis
… in the 3' untranslated region (3'UTR) of their mRNA, thereby targeting them for rapid decay in a process known as ARE-mediated mRNA decay. It has been previously shown that ARE-mediated mRNA decay occurs in cytoplasmic RNA decay centers known as processing bodies (P-bodies). However, the …
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