Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"RNA cleavage"”.
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Biochemical and Structural Studies of the Enzymes Involved in RNA Cleavage and Modification
Part IV: Cleavage of 16S rRNA. Colicin E3 is a highly specific endonuclease that cleaves a single bond in 16S rRNA between A1493 and G1494. This cleavage results in termination of protein synthesis in the cell. In order to determine whether the nucleotide sequence of the target site accounts for …
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PNA-Ligand Bioconjugates as Potential Building Blocks for Sequence-Specific, Metal-Mediated DNA-/RNA-Cleavage
… artificial enzymes for sequence-specific DNA and RNA cleavage is one of the most challenging problems in modern biotechnology, since the commercially available biotools are restricted to a limited number of promoter sequences. Possible nuclease mimics consist of a recognition- and a cleavage …
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In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
"These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… first successful Phase III clinical trial of a RNA interference (RNAi)-based therapeutic is a major achievement in the field. Synthetic RNAi therapeutic oligonucleotides are either first cleaved by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and …
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DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of Dna-Based Biosensors
… many different chemical reactions such as RNA ligation, RNA cleavage, DNA phosphorylation, DNA ligation, DNA adenylation, nucleopeptide formation, Diels-Alder reaction, and porphyrin metalation. RNA ligation is catalyzed by using either an internal 2'-hydroxyl, a terminal 3'-hydroxyl or a …
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Biochemical and Structural Studies of RNA Modification and Repair
"""RNA Repair"" is a mechanism that rectifies purposeful breaks in tRNAs and mRNAs occurred during RNA processing and under cellular stress. To date, only two repair-like and repair pathways have been identified: yeast-type and phage-type, respectively. In our studies, we identified a bacterial RNA …
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CPISPR-CAS: From a Prokaryotic Immune System to a Gene Editing Tool
… associated genes (cas) encode an adaptive, small-RNA-based immune system that protects prokaryotes from infectious phages and plasmids. CRISPR-Cas systems can be classified into three types based on their cas gene content. My thesis work focused on two parts. First, I investigated the mechanism …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
… are involved in gene expression---transcription, RNA processing, and translation. To expand our knowledge of these processes in Archaea, I have studied transcriptional promoters, messenger RNA processing, and 5'-untranslated regions in Methanocaldococcus (Methanococcus) jannaschii. The promoters …
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Nucleoid condensation in Escherichia coli by the DNAbinding protein SymE
… I TA systems consist of a toxic protein and an RNA antitoxin which interacts directly with toxin mRNA to prevent its translation. The toxins of all but two type I TA systems are small proteins that imbed into the inner membrane where they generally oligomerize and form pores that effect membrane …
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Structure, Mechanism and Chemical Modulation of the Protein Kinase-nuclease Dual-enzyme IRE1
… To activate XBP1/HAC1, IRE1 cleaves XBP1/HAC1 mRNA twice to eliminate an inhibitory intron using a dormant nuclease function in its cytoplasmic effector region (IRE1cyto). My focus was to understand the mechanism of XBP1/HAC1 activation by IRE1, the regulation of IRE1 function and the …
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Discovery and biochemical characterization of Casl3b, a Type VI-B CRISPR-Associated RNA-Guided RNase
… of endonuclease protein(s) with targeting guide RNAs that together provide interference against foreign nucleic acids. CRISPR-Cas systems exhibit wide diversity, with the ability to target DNA or RNA, and some large single effector Class 2 Cas proteins have been harnessed for genome engineering. …
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DEVELOPMENT OF E-SRS (ENVIRONMENT-SENSING RESPONSE SYSTEM) AS A NOVEL METHOD TO DISTINGUISH GENETIC ENVIRONMENTS AND RESOLVE CLOSELY RELATED NUCLEIC ACID SEQUENCES
… and response system with two components: 1) An RNA based sensor that changes conformation and activates upon binding specific NTS; 2) A Response System that is triggered by the activated sensor to initiate some physical response, such as emitting a fluorescent signal to indicate presence of the …