Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"RNA chaperone"”.
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
The E. coli RNA degradosome is a complex multi-enzyme machine which is central to the post-transcriptional regulation of the cell. Some of its functions include maturing and processing sRNA, rRNA, and tRNA, as well as degrading mRNA. The key components of the RNA degradosome include the …
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A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi
We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, <em>Borrelia burgdorferi</em>; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the …
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A small RNA regulates RpoS in the Lyme disease spirochete Borrelia burgdorferi
We have identified and characterized the first sRNA, DsrABb, in the Lyme disease spirochete, <em>Borrelia burgdorferi</em>; as well as, identified a non-canonical RNA chaperone Hfq. The alternative sigma factor RpoS (sigma 38 or sigma S) plays a central role in the reciprocal regulation of the …
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Investigating the dynamic nature of biomolecular condensates that support rotavirus replication
… NSP5, which acts as a scaffold, along with the RNA chaperone NSP2 as the major client biomolecule. While viroplasms have been recognised as ribonucleoprotein condensates that serve as replication sites for RVA, many aspects of their formation, functions, and composition remain poorly understood …
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The Characterization of PG0228 in Porphyromonas gingivalis W83
… Studies have shown a number of small non-coding RNAs (sRNAs) have been related to bacterial virulence. Many of these sRNAs require the facilitation of the bacterial Sm-like protein, Hfq, for optimum function. Hfq is a RNA chaperone involved in RNA stability, sRNA function, and polyadenylation. …
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The Response of Staphylococcus aureus to Culture in a Low-Fluid-Shear Environment
… revealed the decreased expression of the RNA chaperone gene, hfq, which parallels the low-shear response of certain Gram-negative microorganisms. This is the first report describing an Hfq association with fluid shear in a Gram-positive organism, indicating that the ability to sense and …
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Phase Separation Mediated Compartmentalisation of Rotavirus Replication
… the disordered protein NSP5 binds the RNA chaperone NSP2, viral RNAs and other viral proteins to form replication factories known as viroplasms. Early in infection, these conden- sates are liquid-like and dynamic, whereas at later stages they undergo maturation that coincides with …
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Local and global investigations into DEAD-box protein function
… cellular processes, such as gene regulation and tRNA processing, are carried out by structured RNAs. While in vitro most RNAs become kinetically trapped in non-functional misfolded states that render them inactive on a biologically-relevant time scale, RNAs folding in vivo do not share this same …
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Understanding small RNA-mediated post-transcriptional regulation of Salmonella pathogenicity island 1 activator HilA in Salmonella typhimurium
… level, and we have identified a number of small RNAs (sRNAs) that control the SPI1 regulatory circuit. The transcriptional regulator HilA activates the expression of the genes encoding the SPI1 T3SS structural and primary effector proteins. The hilA mRNA 5' untranslated region (UTR) is ~350 …
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The role of post-transcriptional regulators in pathogenesis and secondary metabolite production in Serratia sp. ATCC 39006.
… hypothesized that Hfq-dependent small regulatory RNAs (sRNAs) might also play a role. Hfq is an RNA chaperone involved in post-transcriptional regulation that plays a key role in stress response and virulence in other bacterial species. An S39006 ∆hfq mutant was constructed and in the mutants …
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Identification and Characterization of the Hfq protein and small RNAs in Francisella novicida
… were used to both determine the role of the RNA chaperone protein Hfq and identify potential novel small RNAs in <italic>F. tularensis</italic> subsp. <italic>novicida</italic> strain U112. The Hfq protein is recognized as an important regulatory factor in a variety of cellular processes, …
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Mapping RNA Binding Surfaces on Hfq Using Tryptophan Fluorescence Quenching
… a pleiotropic posttranscriptional regulator and RNA chaperone that facilitates annealing of trans-encoded sRNA/mRNA pairs. It regulates many different cellular pathways including environmental stress responses, quorum sensing, virulence and maintenance of membrane integrity. Hfq is a member of …
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Target regulation and prioritization by the small RNA SgrS in Escherichia coli
Regulation of gene expression by small non-coding RNAs is ubiquitous in all domains of life. In bacteria, small RNAs are known regulators of various stress responses. Diverse mechanisms employed by small RNAs demonstrate multi-faceted nature of gene regulation, tailored to respond to stress with …
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Role of <i>bax</i>, <i>ibpA</i>, <i>ibpB</i> and <i>cspH</i> Genes in Protecting CFT073 (Uropathogenic <i>Escherichia coli</I>) Against Salt and Urea Stress
… hypothetical protein), IbpAB (small heat shock chaperone proteins) and CspH (speculated to be DNA/RNA chaperone protein) were chosen for the current study in which their role in protecting CFT073 against salt or urea stress was investigated. First <i>bax</i>, <i>ibpAB</i> and <i>cspH</i> gene …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
The fate of a bacterial RNA transcript is controlled by three key players: ribonucleases, RNA binding proteins (RBPs) and small regulatory RNAs (sRNAs). Together these form an information-rich regulatory network that exercises post-transcriptional control of gene expression. A key RBP in bacteria …