Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"RNA binding domain"”.
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NMR Studies of the RNA Binding Domain of U1A protein and its Complexes with RNA
Eukaryotic splicing is catalysed by five RNA protein complexes called snRNP's. U1A is one protein component of the U1 snRNP particle. The role of U1A in splicing is unclear, but it does bind a hairpin loop from U1 RNA. U1A also binds two internal bulges found in the 3' untranslated region of the …
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Organization of RNA Transcription Units in Amphibian Oocytes
… loops is investigated. Interestingly, the RNA binding domain of the protein is not required for recruitment specificity to the loops. Instead, a poorly characterized portion of the C-terminus of the protein is required for this process.
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Analysis of Protein and RNA Interactions of the HIV-1 rev Protein
… nucleus via it’s nuclear localization sequence/RNA binding domain and interacts with a ≈234 nt region of viral RNA, termed the Rev Response Element (RRE), which is located in the <em>env</em> region of unspliced and singly-spliced HIV-1 mRNA. The Rev nuclear export sequence (NES) then mediates …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
Ribonuclease III (RNase III) is a double-stranded-RNA-specific nuclease of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the …
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
The RNA recognition motif (RRM) is the most abundant RNA binding domain that is found in all organisms. RRM-containing proteins participate in most steps of gene expression, including translation, splicing, modification and transport of RNA. This dissertation aims to help understand the …
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Role of N-Terminal Region in Modulating RNA Binding Activity of the La Module in Vertebrate LARP6
… known, with most previous work focusing on the RNA binding domain La Module. But previous studies have shown that the N-terminal region (NTR) of LARP6 appears to play a role in RNA binding activity and specificity, as well as being suspected of interacting with the La Module domain. To further …
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Characterization of the Roles of Intrinsically Disordered Regions from RNA-Binding Proteins in Phase Separation
… are membrane-less organelles that enrich RNA and RNA-binding proteins containing intrinsically disordered regions (IDRs). I demonstrate that IDRs, coupled with RNA binding domain and RNA, can phase separate in vitro, producing dynamic liquid droplets. Over time, these droplets mature into …
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
… proteins (LARPs) contain a conserved bipartite RNA binding domain, called the La module, which consists of the La motif and an RNA recognition motif (RRM). Across the LARP subfamilies, the La module interacts with a variety of RNAs, allowing this protein superfamily to perform roles as chaperone …
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Mechanisms of Human LARP6 Nuclear Export
… the expression of type I collagen synthesis by binding to the encoding mRNA. However, the molecular mechanism of ligand binding and cellular localization is not fully understood. Understanding the mechanisms of LARP6 regulation requires knowledge of where and how LARP6 functions inside the cell. …
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Genetic Diversity And Evolution Of Disease Response Genes In Sorghum Bicolor L. Moench And Other Cereals
… alleles, while a peroxidase and a gene with a RNA binding domain have significantly reduced diversity suggesting a recent selective sweep. Finally, we show that sorghum DRGs are significantly closer to the telomere and have more exons than a control set of evenly expressed genes. The evidence …
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Fragile X Mental Retardation Protein Induces Synapse Loss Through Acute Postsynaptic Translational Regulation
… X Mental Retardation Protein (FMRP). FMRP is an RNA-binding protein that plays a complex role in translational regulation. FMRP may be an important regulator of dendritic protein synthesis, which occurs at or near synapses in response to synaptic activity. Many types of long-term synaptic change …
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The PUF proteins in Trypanosoma brucei
… the post-transcriptional level. Proteins with an RNA-binding "Puf" domain are important for post-transcriptional control by modulation of mRNA stability and regulation of translation in other species. This suggested that Puf domain proteins might also have a similar role in trypanosomes. In …
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Protein engineering for cancer therapy
… multi-agent protein-based system for targeted siRNA delivery that provides potential advantages over other nanoparticle- and proteinbased delivery vehicles. In the first agent, the double stranded RNA binding domain (dsRBD) of human protein kinase R is used as an siRNA carrier, in fusion proteins …
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The regulation and function of mRNA cap methylation in pluripotency and differentiation
… the N7-methylguanosine cap at the 5’ end of pre-mRNA occurs co-transcriptionally and is catalysed by a series of enzymes including the N7 RNA methyltransferase (RNMT), which along with its recently discovered activating subunit RAM, methylates the cap. RAM, which contains an RNA binding domain, is …
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Structure-Guided Development of Antifungal Protein Farnesyltransferase Inhibitors and DNA Polymerase Engineering
… neoformans FTase (CnFTase) shows a distinct binding mode from the starting compound, explaining the inhibition mechanism. Additionally, the compound does not exhibit significant mammalian cell toxicity up to 200 µM in cell based assays. We also derivatized and evaluated another human FTase …
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NMR Studies on Termination/- Antitermination Complexes of HIV-1 Transcription
After HIV enters the host cell the viral RNA is reverse transcribed into double stranded DNA and then integrated into the host genome. At this step, transcription of HIV RNA by Rpol II is tightly regulated by several cellular factors including NELF, DSIF, and pTEFb, and the virus encoded Tat …
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Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
AU-Rich Elements: ARE) are cis-acting RNA sequences in the 3'UTRs of a wide range of transcripts that function to regulate mRNA stability, localization, and/or translation through interaction with ARE-Binding Proteins. Apobec-1 Complementation Factor: ACF) was originally identified as a co-factor …
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Unique Features of PTB RRMs: Insight into Protein Motions and RNA Binding
Polypyrimidine tract binding protein: PTB) is a highly conserved RNA binding protein comprised of four RRMs: RNA recognition motifs). RRMs are extremely prevalent in all kingdoms of life, and have been very well characterized in terms of structure and RNA binding properties. However, all four RRMs …
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Investigation of the roX RNAs and the RNA Helicase MLE in Dosage Compensation in Drosophila melanogaster
… and is composed of the two noncoding roX (RNA on the X) RNAs and at least five proteins, including the RNA helicase Maleless (MLE). MLE contains two highly conserved double stranded RNA binding domains (DRBDs) at the N terminus. We investigated the roles of the roX RNAs and MLE helicase …