Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"RNA backbone"”.
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RNA Backbone Rotamers and Chiropraxis
RNA backbone is biologically important with many roles in reactions and interactions, but has historically been a challenge in structural determination. It has many atoms and torsions to place, and often there is less data on it than one might wish. This problem leads to both random and systematic …
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The synthesis and in vitro evaluation of chemically modified siRNAs that contain internal azobenzene derivative spacers for selective and tunable photocontrol of activity
Short interfering RNAs (siRNAs) are biopolymers that are used as post-transcriptional gene regulators and as a natural endogenous defense against attack from viruses. These molecules hold a great deal of interest as pharmaceuticals for the knockdown of unregulated genes, such as in cancers. Through …
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Analysis and Error Correction in Structures of Macromolecular Interiors and Interfaces
… predictions, a better defined library of RNA backbone conformers for validation and building 3D models, and knowledge-based automatic correction of errors in protein sidechain rotamers. </p><p>My study of protein--protein interfaces identifies amino acid pairing preferences in a set of 146 …
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The reaction kinetics and three-dimensional architecture of a catalytic RNA
… promote a reaction similar to a single step in RNA polymerization: attack of a primer 3'-hydroxyl on a 5'-triphosphate, with formation of a new 3'-5' bond and release of pyrophosphate. Derivatives have been shown to catalyze general primer extension reactions, making the ligase a useful paradigm …
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Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
… acid (DNA) and Ribonucleic acid (RNA) backbone, lipid and protein. The cells of HaCaT and ORL-48 microtissues were viable and they were characterised by different nucleus size. Replating experiment demonstrated that the cells in the microtissues could spread and proliferate in the …
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Towards Optimising Lentiviral Vectors Through Structure Informed Genome Modification
… particles carry two copies of transfer vector RNA that become linked via a process known as dimerisation. I focused on improving the infectivity of vectors by targeting their dimerisation and packaging properties based on the hypothesis that WT HIV-1 regulates genome encapsidation tightly by …