Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"RNA aptamer"”.
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Development of an RNA aptamer to PD173955N through SELEX
… them less desirable for medical applications.;Aptamers are newly developed reagents and their utilization in medicinal applications is so far very limited. However their characteristics make them perfect tools to help solve problems associated with PD173955. Here we report a successful …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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Optimizing a protein-RNA aptamer gene regulatory system using an engineered peptide library
… interaction between proteins and proteinbinding RNA aptamers, takes advantage of the reversible interaction between TetR and its RNA aptamer binding partner 5-1.2 to modulate gene expression. With no tetracyclines present, TetR preferentially binds to aptamer 5-1.2 in the mRNA of a gene of …
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Encoded synthesis and evolution of clinically approved 2'-modified ribonucleic acids
… nucleic acid chemistries, such as 2'-O-methyl-RNA (2'OMe-RNA) and 2'-O-2-methoxyethyl-RNA (MOE-RNA), have shown promise in clinical applications due to their enhanced properties like high antisense-binding affinity and increased biostability. However, conventional phosphor-amidite synthesis for …
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Alternative mechanisms of translation initiation in modulation of gap junctional coupling
… including cardiac disease and cancer. The GJA1 mRNA undergoes alternative translation initiation to yield a truncated Cx43 isoform, GJA1-20k, that can regulate gap junction formation. Using epithelial-mesenchymal transition (EMT) as a cellular model of gap junction remodeling, we have …
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Molecular Sensing with Protein and Solid-State Nanopores
… molecules of biological interest, such as DNA, RNA and proteins. Of particular appeal is this technique's ability to probe these molecules without the need for chemical modification or labeling, to do so at physiological conditions, and to probe single molecules at a time, allowing the …
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Structural and functional complexity in synthetic cells from amphiphilic DNA nanostars
… of these structures makes a biological cell internally complex and enables intricate cellular functionality. It follows that those seeking to build synthetic cells would aim to replicate such architectures to incorporate structural and functional complexity into their constructs. The bottom-up …
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Development of novel RNA- and protein-based cancer therapeutics by either silencing of potential oncogenes or active restoration of a tumor suppressor gene
… selectively targets and kills the diseased cell.APTAMER-SIRNA-TRANSCRIPTSA prerequisite for successful RNAi-based cancer therapy is the identification of potent siRNA sequences that selectively silence genes crucial for tumor cell survival. Here the challenge was addressed by silencing key …
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Cell surface receptor specific delivery of short interfering RNAs via bivalent aptamer-siRNA transcripts or covalent antibody-siRNA conjugates
RNA interference (RNAi) represents an evolutionary conserved mechanism for the posttranscriptional regulation of gene expression first discovered in plants and flatworms. In higher eukaryotes posttranscriptional gene silencing is mediated by short interfering RNAs (siRNAs) that induce the sequence …