Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"RNA Recognition Motif"”.
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
The RNA recognition motif (RRM) is the most abundant RNA binding domain that is found in all organisms. RRM-containing proteins participate in most steps of gene expression, including translation, splicing, modification and transport of RNA. This dissertation aims to help understand the …
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Investigation of the dynamics and conformational changes of the U1A protein
The U1A-SL2 RNA complex is a model system for studying interactions between RNA and the RNA recognition motif (RRM), which is one of the most common RNA binding domains. Despite extensive studies conducted to study the origins of U1A-RNA affinity and specificity, little is known about the kinetics …
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Strukturelle und funktionale Analyse des DAZL1-Locus in Mammalia
… die funktionale Domäne des DAZL1-Gens, die RRM (RNA Recognition Motif)-Dömäne herausgestellt. Auch hier findet man die größten Übereinstimmungen innerhalb von Altweltaffen und Mensch. Auch zwischen den RRM-Domänen von Maus und Rind i
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
… proteins (LARPs) contain a conserved bipartite RNA binding domain, called the La module, which consists of the La motif and an RNA recognition motif (RRM). Across the LARP subfamilies, the La module interacts with a variety of RNAs, allowing this protein superfamily to perform roles as chaperone …
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Mechanisms of Human LARP6 Nuclear Export
… I collagen synthesis by binding to the encoding mRNA. However, the molecular mechanism of ligand binding and cellular localization is not fully understood. Understanding the mechanisms of LARP6 regulation requires knowledge of where and how LARP6 functions inside the cell. Previous work suggested …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
"The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Essential Protein Factors in pre-mRNA splicing : A Structural Study by Nuclear Magnetic Resonance Spectroscopy
… proteins that play important roles in eukaryotic RNA splicing. These results are discussed in Chapters 1 and 2, while biomolecular NMR techniques that were employed for the structure determination are outlined in Chapter 3. Materials and methods are described in Chapter 4. Chapter 1 presents the …
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Rbfox1 Regulates mRNA Translation to Promote Germ Cell Differentiation
… until it forms a mature oocyte. Drosophila RNA-binding Fox-1 (Rbfox1), also known as Ataxin-2 Binding Protein 1 (A2BP1), mutant females exhibit a germ cell differentiation defect that results in germline cystic tumors. The Rbfox genes encode several isoforms, many of which contain a highly …
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Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro
<p>The unique C-terminal domain (CTD) of RNA polymerase II (RNAPII), composed of tandem heptad repeats of the consensus sequence YSPTSPS, is subject to differential phosphorylation throughout the transcription cycle. Several RNA processing factors have been shown to bind the appropriately …
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Effects of BRCA1-Mediated Ubiquitination of HuR on RNA Metabolism in Breast Cancer
… dissertation examines the ubiquitination of the RNA binding protein Human antigen R (HuR) by BRCA1/BARD1 and the implications of this ubiquitination event on gene expression in breast cancer.</p> <p>HuR binds to mRNA targets in the nucleus and escorts them to the cytoplasm, typically stabilizing …
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Molecular and functional characterisation of circadian clock regulated RNA-binding proteins from Arabidopsis thaliana
The clock-regulated small RNA-binding protein AtGRP7 (Arabidopsis thaliana glycinerich RNA-binding protein) influences its own circadian oscillations by negative autoregulation at the post-transcriptional level, presumably by binding to its own transcript. The present work analyses the RNA-binding …
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Principles of HuR-RNA targeting, interaction dynamics, and functional outcomes
… of the organizational logic of gene expression. RNA-binding proteins (RBPs) and non-coding RNAs are the regulatory molecules primary responsible for interaction with target mRNAs and thereby regulating post-transcriptional processes eventually influencing characteristics of the encoded protein. …
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Elucidating the structure-function relationship of the cell-type specific splicing regulator, RBPMS
… a crucial role in regulating various aspects of RNA metabolism, including splicing. The RNA binding protein, RNA binding protein with multiple splicing (RBPMS), regulates the alternative splicing programme in differentiated vascular smooth muscle cells (VSMCs). RBPMS has an N-terminal RNA …
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Role of Non-Coding RNA NC4 in MLL and CYP33 Mediated Regulation of HOXC8
… Homeo Domain (PHD) of MLL via its N terminal RNA Recognition Motif (RRM) such that its overexpression switches the function of transcriptional activator protein MLL towards transcriptional repressor. This repression is mediated in part by an enhanced recruitment of histone deacetylase 1 to MLL …
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Characterization of split ends, a new component of the Drosophila epidermal growth factor receptor signaling pathway
… acids. It contains three tandem repeats of an RNA Recognition Motif (RRM) at its N-terminus, suggesting that Spen might function as an RNAbinding protein. Spen also contains a highly conserved SPOC (Spen Pearalogue and Orthologue C-terminal) domain at its C-terminus. Spen-like proteins exist …
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Interaction of Spliceosomal U2 snRNP Protein p14 with Its Branch Site RNA Target
<p>Newly transcribed precursor messenger RNA (pre-mRNA) molecules contain coding sequences (exons) interspersed with non-coding intervening sequences (introns). These introns must be removed in order to generate a continuous coding sequence prior to translation of the message into protein. The …
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Warten auf die Transkription: Die humanen Elongationsfaktoren NELF und DSIF
… Dabei interagieren beide Faktoren direkt mit RNA-Polymerase II. NELF bindet über die RNA recognition motif (RRM)-Domäne der Untereinheit E zusätzlich an die naszierende RNA. Eine derartige Transkriptionsblockade tritt auch während der Transkription des Genoms des humanen Immunschwächevirus 1 …
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Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
AU-Rich Elements: ARE) are cis-acting RNA sequences in the 3'UTRs of a wide range of transcripts that function to regulate mRNA stability, localization, and/or translation through interaction with ARE-Binding Proteins. Apobec-1 Complementation Factor: ACF) was originally identified as a co-factor …