Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"RNA Polymerase I"”.
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In Vivo Studies of Ribosomal RNA Transcriptional Regulation within a Drosophila Stem Cell Lineage
As the first step of ribosome biogenesis, RNA polymerase I-directed ribosomal RNA gene (rRNA) transcription is critical for cell growth, proliferation and cell survival. Upregulated rRNA levels have been observed in many types of cancers. However, the extent to which rRNA transcription is …
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Proteomics and dynamics of the human nucleolus
… to be the site where the majority of ribosomal RNAs (5.8S, 18S and 28S) are transcribed, processed and assembled with ribosomal proteins to form ribosomal subunits. The sole role of ribosome biogenesis, however, cannot explain the specific nucleolar localisations of tumour suppressors, cell …
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Zur funktionellen Architektur des Nukleolus in lebenden Zellen
… Im einzelnen wurde durch die Untersuchung von RNA-Polymerase I der rDNA Transkriptionsort im fibrillären Zentrum des Nukleolus bestätigt. Die kinetischen Analysen von zwei pol I-Untereinheiten (RPA194 und RPA53) durch FRAP in transkriptionell aktiven und inaktiven Nukleoli erlaubten direkte …
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Die Rolle des Proteins DEDD in Apoptose
… Experimente zeigten, daß DEDD spezifisch die RNA Polymerase I-abhängige Transkription in den Nukleoli inhibiert. Zudem aktiviert DEDD spezifisch Caspase-6 im Nukleus und kolokalisiert dort mit der aktiven Form des Enzyms. DEDD benötigt eine funktionelle DED, um pro-apoptotisch zu wirken, sich …
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Mechanism and regulation of ribosomal RNA transcription
The sole responsibility of RNA polymerase I (pol I) is the transcription of the ribosomal RNA (rRNA) genes. This transcription can account for up to 60% of the total cellular RNA that is being transcribed in an actively dividing cell. Due to the large amounts of energy this transcription requires, …
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A Unified View of Protein Low-complexity Regions (LCRs) Across Species
… the importance of K-rich LCR copy number for the RNA polymerase I subunit RPA43 for both assembly in vitro and localization in cells, demonstrating how principles of LCR copy number can relate these two processes. Moreover, by mapping regions of LCR sequence space to higher-order assemblies, such …
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Genetic Determinants of Junin Virus Attenuation
… effective and safe vaccines. To this end, an RNA polymerase I/II-based reverse genetics system was utilized to rescue the wild type pathogenic Romero and attenuated Candid #1 strains of JUNV. Both recombinant viruses exhibited similar in vitro growth kinetics and in vivo biological properties …
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Funktionelle Charakterisierung der CK2-vermittelten Phosphorylierung des Transkriptionsfaktors TIF-IA bei der Regulation der Synthese ribosomaler RNA
In Säugern wird die ribosomale RNA von RNA-Polymerase I (Pol I) synthetisiert. Die Interaktion der Pol I-spezifischen Faktoren TIF-IA und TIF-IB miteinander sowie die Bindung von TIF-IB an den rDNA-Promotor sind für die Transkriptionsinitiation notwendig. Ein Guanin an Position -7 (G-7) im core …
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VSG expression in African trypanosomes: Gene silencing, cell cycle and developmental control
… (ESAGs) in a polycistronic transcription unit by RNA-polymerase I, from one of approximately 15 telomeric VSG expression site (VSG-ES). This produces super-abundant transcript and protein; approximately 10 % of the cells gene expression is dedicated to this single gene. In the tsetse fly, VSG …
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Identifying individual ribosomal RNA gene repeats to investigate their behaviour
Ribosomal RNA forms the major structural and catalytic components of ribosomes. In eukaryotes, the ribosomal RNA genes (rDNA) are present as multiple tandem repeats that are transcribed by a dedicated polymerase, RNA polymerase I. While hundreds of rDNA repeats are present, only a subset is …
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Functional genetics reveals surprises about the mechanisms of action of chemotherapeutics
… cells via DNA cross-linking. However, using an RNAi-based drug mechanism of action predictive signature, we were able to determine that oxaliplatin instead exerts its cytotoxicity via inhibition of transcription/translation, not the canonical DNA crosslinking mechanism of action exhibited by …
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Molecular evolution of the RNA polymerase genes and the phylogeny of seed plants
… done using chloroplast gene rbcL and nuclear rRNA genes. While these molecular markers revolutionized plant systematics, they do not contain enough phylogenetic information to resolve the phylogeny of seed plants. I used normalized splits, as a measure from spectral analysis, in examining data …
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Molecular Characterisation and Therapeutic Targeting of NPM1-mutant Acute Myeloid Leukaemia
… progenitors displayed increased sensitivity to RNA polymerase I inhibitors, such as Actinomycin D (ActD). The combination of ActD and Venetoclax inhibited the growth and colony forming ability of pre-leukaemic and leukaemic NPM1c+ cells and low-dose ActD treatment was able to re-sensitise …
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Dynamics of human DNA Topoisomerases I and II
… and consistently colocalizes there with RNA-polymerase I. Finally, we show that certain cancer drugs believed to act by stabilising covalent catalytic DNA-intermediates of topoisomerases, do indeed immobilize the enzymes in living cells. Interestingly, these drugs do not target …
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Making Ribosomes: Biochemical and Structural Studies of Early Ribosome Biogenesis in Yeast
… the cell. In yeast, it is made of four ribosomal RNAs and 79 proteins, asymmetrically divided in a small and large subunit. In a growing yeast cell, more than 2000 ribosomes are assembled every minute. The ribosome is assembled through a highly complex process involving more than 200 trans-acting …
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Transcriptional regulation in Plasmodium falciparum: characterisation of TBP-like protein
… factor required for transcription by RNA polymerase I, II and III. Multiple gene duplication events throughout eukaryotic evolution have resulted in many eukaryotes harbouring more than one TBP family protein. In metazoans, TBP paralogs have been shown to play important roles in …
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Matrix Gene Splicing and Emergence of Highly Pathogenic Avian Influenza
… viruses were capable of producing messenger RNA 4 (mRNA4), which had direct implications in the emergence of virulent viruses from this lineage. The avirulent viruses expressed mRNA4 at high levels. We demonstrated that there was strong selection for virulent viruses with weaker mRNA4 splice …
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Expression and behaviour of GPI-anchored surface proteins in trypanosomes
… telomeric expression site and is transcribed by RNA polymerase I. VSG is an essential protein, knockdown of the active VSG mRNA using RNAi results in a pre-cytokinesis cell cycle arrest, resulting from a threshold requirement of VSG for cellular proliferation. It is not known how the cell …