Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 26 for “"RNA Biology"”.
-
Bridging organic synthesis and RNA biology: total synthesis of sannamycin B and reversible RNA acylation
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-08-01
-
Mechanisms of transcription and translation dysfunction in disease: a novel FMRP-translation axis in Alzheimer’s and expansion of Pol III transcription in cancer
… across multiple layers, from chromatin state to RNA processing and translation. Crucially, a gene encodes an RNA molecule before it ever becomes a protein, and RNAs themselves are diverse in form and function. Dysregulation of RNA pathways is implicated in many diseases, like cancer, neurological …
-
Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
Cell surface RNA (csRNA) is a recent discovery in the field of RNA biology and has been implicated in playing important roles in many biological processes due to its extracellular properties. To understand the biogenesis, regulation, and function of csRNA, it is critical to develop methods to …
-
Discovery and characterization of Cas13b, a differentially regulated RNA-targeting CRISPR system
RNA plays a significant role in human biology and disease, not only as messenger RNA encoding proteins but also as noncoding RNA regulating DNA, proteins, and other RNA species. Until recently, it has been challenging to target RNA in a simple, efficient manner. CRISPR-Cas (clustered regularly …
-
Discovery of novel CRISPR enzymes for transcriptome engineering and human health
RNA plays important and diverse roles in biology, yet molecular tools to measure and manipulate RNA are limited. Recently, the bacterial adaptive immune system, CRISPR, has revolutionized our ability to manipulate DNA, but no known RNA-targeting versions exist. To discover parallel bacterial …
-
Elucidating the role of SRSF1, a prototypical splicing factor, in liver physiology
… has revealed it plays multiple roles in RNA biology. This ranges from mRNA transport, translation regulation, non-sense mediated decay and even processing of miRNA. Therefore, it is not surprising that SRSF1 is vital for the survival of the cell. While deficiency in most tissues results …
-
Yeast Ataxin-2 (Pbp1) Condensates Regulate TORC1 Activity and Autophagy in Response to Cellular Redox State
… been implicated in stress granule formation, RNA biology, and neurodegenerative disease. However, the normal endogenous function of Pbp1 and ataxin-2 remains poorly understood. In this dissertation, I identified Pbp1 as a dedicated regulator of TORC1 signaling and autophagy under conditions …
-
Generative Machine Learning Models for RNA Structure Prediction and Design
Ribonucleic acid (RNA) is a fundamental molecule in biology, central to the regulation and execution of life’s most essential processes. Its diverse roles range from encoding genetic information to catalyzing biochemical reactions. Beyond its modern biological functions, RNA is also believed to …
-
Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
-
Computational Modeling of Rna-Small Molecule and Rna-Protein Interactions
<p>The past decade has witnessed an era of RNA biology; despite the considerable discoveries nowadays, challenges still remain when one aims to screen RNA-interacting small molecule or RNA-interacting protein. These challenges imply an immediate need for cost-efficient while predictive …
-
Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… in glass nanopore sensing for both DNA and RNA analysis by leveraging nucleic acid self-assembly, all without requiring enzymatic amplification or fluorescence labelling. To establish a fundamental understanding of signal generation mechanisms, I first conducted a systematic investigation …
-
Characterisation of RNA modifications in human cancer cells
… have been studied as key modulators of RNA biology for over 50 years. There are now 171 RNA modifications that have been discovered throughout the three kingdoms of life1. These chemical changes overlay the standard RNA information and have been linked to a wide range of biological …
-
THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES
… a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been …
-
Molecular Genetic Analysis of Tumor Cells and Developmenet of RNA Interferance Therpy in Neurofibromatosis Type1(NF1)
… mechanism may be exploited therapeutically. RNA interference (RNAi) is a newly discovered and popular technology platform among researchers not only in the fields of RNA biology and molecular cell biology. It has created excitement in clinical sciences such as oncology, neurology, …
-
Assessing and understanding the generation and function of RNA decay intermediates in non-insect borne flaviviruses
… correctly to stimuli or to maintain homeostasis. RNA viruses must act to preferentially drive production of their own messenger RNAs (mRNAs) and proteins in order to successfully replicate and ensure continued infection. Due to the necessity for RNA viruses to remain in the cytoplasm, regulatory …
-
Role of RNA methyltransferases in lung cancer
In recent years, RNA-modifying enzymes have gained significant attention because their activities are relevant to cancer biology, and they are therefore potential targets for novel therapeutic intervention. Here, I target validated two RNA methyltransferases. I performed a late-stage study of …
-
Mechanistic insights into endoribonuclease activation of the mRNA 3' end processing machinery by Cleavage Factor IA
Pre-mRNAs must undergo a series of cotranscriptional processing steps for efficient export from the nucleus, translation and mRNA stability. Near the 3' end of the nascent transcript, the pre-mRNA is cleaved at specific RNA sequences, releasing the pre- mRNA from the transcribing RNA polymerase II. …
-
Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… process. Regulation of genes transcribed by RNA polymerase II is coordinated by an elegant arrangement of protein machinery and chromatin architecture, and the resulting pre-mRNA is post-transcriptionally processed and regulated at every step to buffer, reduce, or amplify its protein output. …
-
Investigating Mechanisms of Nuclear mRNA Export
In eukaryotes, the segregation of the RNA synthesis/processing in the nucleus from the translation machinery in the cytoplasm has important implications for nuclear export and retention of mRNAs. This division ensures that only correctly processed mRNAs are exported to the cytoplasm to be …
-
Understanding the physiological role of the small RNA RydC
… understand the physiological role of the small RNA (sRNA) RydC and the signals controlling RydC production. RydC was previously shown to stabilize cfa (cyclopropane fatty acid synthase) mRNA, resulting in higher levels of cyclopropane fatty acids in the cell membrane. Here, we show that …
Page 1 of 2