Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"RNA -- Analysis"”.
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THE VASCULAR RESPONSE WITHIN THE ISLET DURING BETA CELL DEVELOPMENT AND REGENERATION
… of β cells. Immunohistochemical and quantitative RNA analysis was performed to observe the ontogeny of changes to microvasculature, endocrine tissue, and growth factors over the subsequent 40 days. Normal islet development involved remodelling of the islet vasculature around weaning which may …
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The Regulation of the Phosphatidate Phosphatase Gene <em>PAH1</em> and Its Regulatory Role on Cell Homeostasis
… WT in the presence of inositol. Furthermore, our RNA analysis showed that UAS<em><sub>INO</sub></em> genes were upregulated in the <em>pah1∆</em> strain when compared to WT cells, strongly suggesting the role of <em>PAH1</em> as a negative regulator in the lipid biosynthesis pathway. …
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Analysis of RNA stability in forensic specimens
Ribonucleic acid (RNA) analysis presents a unique, novel opportunity to answer a wide range of questions in forensic science. The dynamicity of the transcriptome has led to it being suggested as a novel source of diagnostic information in forensic pathology. However, RNA is exceptionally labile. …
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Single-Molecule Characterization of RNA via Nanopore Sensing
Precise characterization of RNA is essential for understanding a wide range of biological processes, studying gene expression, developing new RNA-based therapeutic technologies, and elucidating disease mechanisms. However, current RNA characterization methods often provide an incomplete picture of …
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Optimization of Molecular Beacon-Based Multicomponent Probes for Analysis of Nucleic Acids
… single nucleotide substitutions (SNS) in DNA and RNA has a growing importance in biology and medicine. One traditional approach for recognition of SNS takes advantage of hybridization probes that bind target nucleic acids followed by measuring ?Tm, the difference in melting temperatures of matched …
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The mRNA Cap and Discontinuous Transcription in Trypansoma brucei
… phylogenetic order, Kinetoplastida, synthesize mRNA by a novel pathway that joins RNAs from physically unlinked gene segments. This results in the presence of a short non-coding RNA leader, called the miniexon, at the 5' end of most, probably all, mRNA. Indirect evidence suggests that …
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Development of a framework for multiomic analysis in disease research
… Medicine Centre (NIGMC), 815 PAXgene (for RNA analysis) and 738 buffy coats (for DNA and epigenetic analysis) were collected and stored, with the intention of further multi-omic analysis where whole genome sequencing by the 100KGP was insufficient to provide a diagnosis for patients with …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
Post-transcriptional RNA modifications, such as N6-methyladenosine (m6A), 2'-O-methylation (Nm), and 5-methylcytosine (m5C), are critical regulators of gene expression. Mapping these modifications at single-nucleotide resolution, however, remains a major challenge as current methods often suffer …
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The microaerophilic nature of <i>Wolinella recta, Wolinella curva, Bacteroides ureolyticus</i>, and <i>Bacteroides gracilis</i>
… among bacteria can be identified by ribosomal RNA analysis, but the resulting groups may not be easily definable by phenotypic characteristics. This is exemplified by the genus <i>Campylobacter</i>, which consists of at least three separate groups that cannot be differentiated readily by …
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Comparative analysis of transcriptome of fracture healing under normal and hypophosphatemic conditions
… in bone regeneration. This transcriptomic analysis investigates the intricate relationship between environmental effects of phosphate deficiency on the time course of fracture healing within the C57BL/6J (B6) mouse strain. To manipulate the healing environment, a low phosphate diet was used …
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Regulation of floury2-mRFP expression in response to long term selection for grain protein concentration in maize
… many genes with small phenotypic effects. An alternative theory explored here is that the response depends on quantitative expression variation of a few major regulators. Analysis of crosses between IHP and Illinois Reverse High Protein (IRHP) provides evidence that the response to selection in …
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Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… in glass nanopore sensing for both DNA and RNA analysis by leveraging nucleic acid self-assembly, all without requiring enzymatic amplification or fluorescence labelling. To establish a fundamental understanding of signal generation mechanisms, I first conducted a systematic investigation …
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DNA chip designed antisense oligodeoxynucleotides targeting EGFR MRNA for brain tumour therapy
… into protein. By specifically binding to mRNA in an antisense manner, they can bring about its degradation by a variety of mechanisms including the activation of RNase H and thus have great potential as therapeutic agents. One of the main drawbacks to the utilisation of this therapy so far …
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Effects of the OTX2ECR2 enhancer & PROX1 overexpression in murine retina development
… photoreceptor markers, or by doing single cell RNA analysis to further elucidate the genetic profile of these cells.</p>
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Application of radioisotope imaging and therapy in patients with breast carcinomas that express the sodium iodide symporter gene
… by immunohistochemistry and ribonucleic acid(RNA) analysis, and this was correlated with functional activity of the NIS protein, by scintigraphic scanning of patients with metastatic breast cancer. Results Twenty-four patients had a Tc99m Pertechnetate scan; 20 patients had their RNA analysed; …
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The Use of 2D and 3D Models to Study Competition in the Context of Barrett’s Oesophagus and Oesophageal Adenocarcinoma
… tagged BO and OAC organoids and imaged for analysis. Fusion events between BO and OAC organoids were captured by wide-field microscopy and quantified, providing an additional method to study competition. In addition, 2D cell-lines with loss of tumour suppressors *p53 and SMAD4* which are …