Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"RNA, Small Interfering"”.
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To Develop a Small Interfering RNA (siRNA) Design and Information Resource to Facilitate Genetic Manipulaton of Human Cells
Part I: Small interfering RNAs (siRNAs) have revolutionized our ability to study the effects of altering the expression of single genes in mammalian (and other) cells through targeted knockdown of gene expression. In the past, there were a set of rules designed to develop siRNA which worked …
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The Impact of Lipid Nanoparticle Chemistry on RNA Delivery and Therapeutic Outcomes
… the ionizable cationic lipid component, impact RNA delivery. To systematically investigate how PEG lipid chemistry impacted LNP formulation and RNA delivery, a series of linear-dendritic poly(ethylene glycol) (PEG) lipids were synthesized with modulated hydrophobic domains. The chemical …
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The Function and Mechanism of RNA Interference in Neurospora
RNA interference (RNAi) is a conserved gene silencing mechanism important for various biological processes, including developmental timing, genome defense, and heterochromatin formation. RNAi is triggered by double stranded RNA (dsRNA), which is processed by Dicer to siRNA. siRNA is loaded onto …
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Engineering Lipid Nanoparticle-Mediated Delivery of Nucleic Acids to Lymphoid Organs and Immune Cells
Ribonucleic acids (RNA) and deoxyribonucleic acids (DNA) hold promise for continued application in the treatment of genetic diseases. Short interfering RNA (siRNA) and microRNA (miRNAs) have been used to silence disease-causing genes. Longer messenger RNA (mRNA) and plasmid DNA (pDNA) have been …
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Using Chemically Modified Oligonucleotides to Modulate Gene Expression, Treat Genetic Diseases, and Probe Novel Mechanisms of RNA Interference
… glutamine-expanded protein. Targeting HTT with siRNAs could be a powerful approach, but allele-selectivity is a major challenge: nearly all HD patients are heterozygous at the HTT locus, and expression of wild-type HTT may need to be preserved. One way to achieve allele-selectivity is by …
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Exploration of Nuclear Receptor Activity and siRNA-Derived Phenotypes as Therapeutics in Non-Small Cell Lung Cancer
… high-throughput biomarker platform capable of mRNA expression signature assessment from FFPE specimens. The platform was used to assess NR/CoReg expression levels across a 500+ sample FFPE dataset. Categorical NR/CoReg downregulation upon tumor progression as well as survival benefits for …