Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"RING-E3"”.
-
Origins and composition of the cullin-RING E3 ligases in trypanosomes
… the E2s and eventually their recruitment to an E3 ligase that will transfer it to the substrate. Cullin-RING is a prominent family of E3 ligases responsible for ubiquitylation of up to 20% of the human proteome. In contrast with the rest of the class, cullin-RINGs perform their function as …
-
Ligandability of protein-protein interactions and surfaces on Cullin RING E3 ubiquitin ligases
Cullin RING E3 ubiquitin ligases (CRLs) function in the ubiquitin proteasome system by catalysing the transfer of ubiquitin from E2 conjugating enzymes to specific substrate proteins. CRLs are large dynamic multi-subunit complexes that control the fate of many proteins in cells and, therefore, …
-
Regulation of BubR1 by Ultraviolet Radiation: Implications in Skin Cancer
… Cullin, F-box) complex, belonging to the Cullin RING E3 ubiquitin ligase (CRL) family of E3 ubiquitin ligases that regulates the stability of a wide range of cellular proteins by recognizing and binding specific substrates through a conserved degron motif and targeting them for ubiquitination and …
-
Development of Protein Degraders Inspired by Chemical Modifications
… that Dha itself can function as the minimal E3 ligase-recruiting handle and that tighter ternary complex formation is favored. Mechanistic elucidation via inhibitor screening confirmed that the Dha-based degrader operates through the ubiquitin-proteasome system by recruiting a Cullin-RING E3 …
-
Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
… Promoting Complex/Cyclosome (APC/C) is a RING E3 ligase that plays essential roles both within and outside of the cell cycle. At the onset of anaphase, the APC/C targets cyclin B and securin for degradation, initiating chromosome separation and mitotic exit. Regulation of APC/C activity is …
-
Design and Synthesis of an E3 ligase Activity-Based Probe and its Application for the Discovery of a New Class of E3 Ligase
… The distinct transfer cascade, involving E1-E2-E3 enzymes, serves as a promising target for drug development. However, E3 ligases (E3s) represent an important class of enzymes yet there are currently no effective tools for profiling their activity. Herein, a new class of E3 activity-based probe …
-
Mechanisms underlying substrate engagement by TRIM7 and TRIM21 and strategies for re-directing their E3 ligase activity
… TRIM7 and TRIM21. Both proteins are ubiquitin E3 ligases that ubiquitinate and degrade substrates. My work on TRIM7 has uncovered a unique molecular mechanism of substrate selection and explains why TRIM7 is reported to interact with unrelated and diverse substrates, ranging from cellular …
-
The Potential of CRL4-DCAF1 and KSR1 as Therapeutic Targets in Low-grade Merlin-Deficient Tumours
… tumour development. The activity of both the E3 ubiquitin ligase complex Cullin 4 really interesting new gene (RING) E3 ubiquitin ligase- DNA damage binding protein (DDB1) and Cullin 4 associated factor 1 (CRL4-DCAF1) and Kinase suppressor of RAS 1 (KSR1) have been shown to be upregulated in …
-
Investigating the role of DA1 in growth control.
… constitutes the harvestable yield, the engineering of larger seeds provides a possible strategy for yield improvement. A detailed understanding of the growth of plant organs in general is paramount if such advances are to be made. Utilising previously characterised regulators of plant organ …
-
Structural Insights into TRIM21 Ubiquitination Mechanisms
… in essentially all physiological processes. During ubiquitination, RING E3 ligases direct E2 Ub-conjugating enzymes to substrates to catalyze their ubiquitination. The cytosolic antibody receptor TRIM21 possesses unique ubiquitination activity that drives broad-spectrum anti-pathogen targeting …
-
Role of the RING domain in MDM2-mediated ubiquitination of p53
… for degradation by the 26s proteasome via its E3 ligase activity. The E3 ligase activity of MDM2 is dependent on its C-terminal RING domain. E3 ligases containing a RING domain are traditionally thought to catalyse the transfer of ubiquitin from their conjugating enzyme (E2) partner to the …
-
Molecular characterization of tomato brown rugose fruit virus-host interactions and development of biocontrol strategies
… degradation via SCFFBS1, a prototypical Cullin-RING E3 ubiquitin ligase complex. Phospho-mimetic assays further revealed that phosphorylated SGT1 and HSP90 negatively regulate MP abundance and ToBRFV accumulation. Application of exogenous salicylic acid (SA) could activate SGT1 and HSP90 through …
-
Protein-Protein Interactions and Small Molecule Targeting of the Multisubunit SOCS2-EloBC-Cul5-Rbx2 E3 Ubiquitin Ligase
The Cullin-RING E3 ligases (CRLs) are the largest subfamily of E3 ligases that participate in many biological processes determining the fate of proteins by catalysing ubiquitin transfer to specific substrates for proteasomal degradation. SOCS2 is a component of the multisubunit CRL E3 complex …
-
Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
DCUN1D1 is an E3 ligase of the neddylation pathway. It mediates the posttranslational modification of majority of the cullin family of proteins with NEDD8. This activity is known to enhance ubiquitination of the cullin RING E3 ligases, however, the extent of the impact of DCUN1D1's activity is …
-
Characterising the mechanism of DCUN1D1 activity in prostate cancer and identifying DCUN1D1 inhibitors for prostate cancer treatment
DCUN1D1 is an E3 ligase of the neddylation pathway. It mediates the posttranslational modification of majority of the cullin family of proteins with NEDD8. This activity is known to enhance ubiquitination of the cullin RING E3 ligases, however, the extent of the impact of DCUN1D1's activity is …
-
The p97 cofactors UBXN7 and UBXN8 interact with and modulate the function of cullin-RING complexes and Fanconi anaemia proteins FANCD2/FANCI, respectively
… most proficient in interacting with CRL (cullin-RING E3 ligase) subunits, in particular CUL2, and it was assumed that these interactions were indirect, mediated by ubiquitylated substrates. However, we show that UBXN7 interaction with CUL2 is independent of ubiquitin- and substrate binding. …
-
Regulation of the Parkin E3 ligase by PINK1-dependent phosphorylation
… onset Parkinson’s disease. Parkin encodes a RING-In Between RING-RING E3 ligase that normally resides in the cytoplasm and is<br/>regulated by autoinhibitory intramolecular interactions. PINK1 is a mitochondrial Ser/Thr kinase that is unique in possessing a N-terminal mitochondrial targeting …
-
COP9 signalosome subunit knockdown in K562 provides novel insight into the function and potential regulation of the CSN
… is mediated by three enzymes; E1, E2, E3. The largest family of E3’s is the cullin-RING E3 ubiquitin ligases (CRLs). CRLs require the cyclic addition and removal of a ubiquitin-like protein called NEDD8 to and from the cullin subunit. Removal of NEDD8 (deneddylation) is mediated by the …
-
TRIM21 Mediated Antibody-Dependent Intracellular Immunity
… I investigated the impact of naturally occurring polymorphisms on the antiviral functions of TRIM21. Interestingly, coding variants in TRIM21 are individually rare and all missense variants tested empirically retained at least some antiviral activity. This suggest that the antiviral functions …