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Showing 1 to 20 of 28 for “"RING domain"”.
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Analysis of The RING Domain And BRCT Repeats of BRCA1
… that may be important for BRCA1's functions: the RING domain and the BRCT repeats. BRCA1's RING domain has E3 ubiquitin ligase activity, which is greatly enhanced when it forms a heterodimer with Bard1. This region is of particular interest because it displays the only known enzymatic activity of …
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Role of the RING domain in MDM2-mediated ubiquitination of p53
… activity of MDM2 is dependent on its C-terminal RING domain. E3 ligases containing a RING domain are traditionally thought to catalyse the transfer of ubiquitin from their conjugating enzyme (E2) partner to the target protein, in the final step of the ubiquitination cascade. Various E2 enzymes …
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Two Redundant Ubiquitin-dependent Pathways of BRCA1 Localization to DNA Damage Sites
… activity of the BRCA1 C-terminal (BRCT) domains is essential for ubiquitin-dependent recruitment and it is through these domains that BRCA1 indirectly interacts with RAP80, a ubiquitin-interacting protein. RAP80 targets BRCA1 to DSBs but whether or not RAP80 is the sole targeting subunit …
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바이러스 감염시 미토콘드리아 E3 ubiquitin ligase 인 MARCH5의 새로운 생체 기능
… to RNA viruses. MARCH5 bound MAVS only during viral stimulation when MAVS forms aggregates, and these interactions required the RING domain of MARCH5 and the CARD of MAVS. MARCH5, but not its RING mutant of MARCH5H43W, reduced the level of MAVS aggregates. MARCH5 transferred ubiquitin to …
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Effects of BRCA1-Mediated Ubiquitination of HuR on RNA Metabolism in Breast Cancer
… BRCA1, together with BARD1 (BRCA1 Associated RING Domain 1) also functions as an E3 ubiquitin (Ub) ligase. BRCA1 mutation in the RING domain, which is responsible for E3 ligase activity, is associated with increased risk of developing breast cancer. Despite extensive research on the activities …
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Design and Synthesis of an E3 ligase Activity-Based Probe and its Application for the Discovery of a New Class of E3 Ligase
… (ABP) is presented which is built by re-engineering ubiquitin (Ub)-charged E2 conjugating enzymes. The utility of these probes has been demonstrated by the rapid dissection of the activation determinants of the RING-Between-RING E3 (RBR) E3, Parkin. Furthermore, biotin-E3 ABPs allow us to …
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Biochemical and Genetic Studies on CC2D1A, a new NF-κB Activator and a Regulator of Synaptic Functions
… belongs to a new protein family with four DM14 domains at the NH2 terminus and a C2 domain at the COOH terminus. The function of this protein family remains largely unknown. CC2D1A has been identified as a new NF-κB activator through a large scale screen of human genes by Matsuda et al. Here I …
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Transcriptional Regulation of Neurogenic Atrophy-Induced Gene Expression by Muscle Ring Finger-1 and Myogenic Regulatory Factors
… in repression of MuRF1 expression, a MuRF1 RING domain mutant and a MuRF1 c-terminal mutant were created. The mutant constructs were then co-transfected along with MRF expression plasmids and the MuRF1 reporter construct into C2C12 cells and reporter gene activity was assessed. The MuRF1 …
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The identification of NRAGE
… indicated that this interaction requires the RING domain. The widely studied 32D cell system was chosen to investigate the effect of NRAGE on apoptosis. NRAGE was stably transduced in 32D cells, and found to augment cell death induced by the withdrawal of Interleukin-3. One reason for this …
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Mechanisms of attenuation of the live Junin virus vaccine strain, Candid #1
… degree. An amino acid substitution within the RING domain of the Candid #1 Z protein results in the protein budding from the cell independently, and there is a significant decrease in interaction between GP and Z in a plasmid expression system. Together, these results demonstrate that the …
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Structural Insights into TRIM21 Ubiquitination Mechanisms
… in essentially all physiological processes. During ubiquitination, RING E3 ligases direct E2 Ub-conjugating enzymes to substrates to catalyze their ubiquitination. The cytosolic antibody receptor TRIM21 possesses unique ubiquitination activity that drives broad-spectrum anti-pathogen targeting …
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ELUCIDATION OF REGULATORY MECHANISMS OF TRANSCRIPTION AND COUPLED mRNA EXPORT
… role of ubiquitylation in mRNA export via HECT domain-containing E3 ligases like Tom1 and Rsp5. My work has revealed the role of an F-box protein (Mdm30) component of RING domain-containing SCF (Skp-Cullin-F-box) family of E3 ligases in mRNA export, thereby implicating the role of ubiquitylation …
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On the function of the interferon-inducible p53 target gene TRIM22
… Motif) family. The TRIM family is a family of RING (Really Interesting New Gene) proteins, defined through a tripartite motif, containing a RING-domain, one or two B-boxes and a Coiled-coil-domain. The TRIM proteins are involved in diverse biological functions, such as apoptosis, cell-cycle- …
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TRIM21 Mediated Antibody-Dependent Intracellular Immunity
… I investigated the impact of naturally occurring polymorphisms on the antiviral functions of TRIM21. Interestingly, coding variants in TRIM21 are individually rare and all missense variants tested empirically retained at least some antiviral activity. This suggest that the antiviral functions …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
… replication and dissemination to neighboring cells. Shigella flexneri and other bacteria including Salmonella and Yersinia secreted E3 ubiquitin ligases into the host cell cytoplasm via the Type III secretion system (T3SS) apparatus. The invasion plasmid antigen Hs (IpaHs) are a novel …
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REGULATION OF EUKARYOTIC TRANSCRIPTIONAL ELONGATION AND ASSOCIATED DNA REPAIR
… monoubiquitination. However, deletion of Bre1p’s RING domain that is essential for histone H2B monoubiquitination led to an impaired RNAPII association with the active gene. This observation indicates a role of the non-RING domain of Bre1p in repressing the association of RNAPII with the active …
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The Bca2 And Ampk Paradigm: Unraveling The Cancer Connection
… Breast Cancer Associated Gene 2 (BCA2/Rabring7/RNF115), a novel RING-finger ubiquitin E3 ligase, is overexpressed in >50% of breast tumors. Herein, I hypothesized that BCA2 is an endogenous inhibitor of AMPK activation in breast cancer cells and that BCA2 inhibition would therefore …
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An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… as with the F1-ATP synthase or accompany domain motions during a conformational change such as the relative rotation of the large and small ribosomal subunits during protein synthesis. Studying these motions can provide insight into the mechanics of enzyme function that cannot be obtained …
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Characterising the interaction between TRIM28 and members of the Krab-ZFP family in transcription silencing
… ERV elements are under tight control during early embryogenesis, largely through TRIM28-mediated transcription repression. TRIM28 (also known as KAP1 or TIF1β) functions as the co-repressor protein for the largest family of transcription factors, namely the Krüppel-associated …
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PHDS, Stress, And Starvation: The Identification Of A New RPD3 Deacetylase Complex Involved In The Yeast Oxidative Stress And Metabolism Pathways
… or repressing transcription. One protein domain involved in targeting chromatin regulators is the plant homeodomain, or PHD finger, a module that preferentially interacts with either methylated or unmethylated lysines on histones, and has important functions in human health. Despite recent …
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