Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"RIBOSOMAL SUBUNITS"”.
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Molecular visualization and localization of ribosomal subunits interaction in cells
… which occurs in the cytoplasm. Ribosome subunits and several translation factors are present in the nucleus but it is understood that they can interact to form the functional ribosome only during translation in the cytoplasm. Recent studies, however, have suggested that translation may …
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The Role of CASK in Central Nervous System Function and Disorder
… as synaptic proteins, metabolic proteins, and ribosomal subunits. Thus, absence of CASK in half of brain cells seems to affect synaptic protein content, cell metabolism, and protein homeostasis. Extending these observations, I conducted GFP-trap immunoprecipitation followed by tandem mass …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
… apparent block in the nucleolar release of large ribosomal subunits in the terminally differentiated larval midgut polyploid cells. Depletion of 60% of NS1 transcripts lead to larval and pupal lethality. Ultra-structural examination of highly differentiated larval Malpighian tubule cells depleted …
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Exploring conformational variability of an rna domain in the ribosome: from structure and function to potential antibiotic targeting
… to expand the genetic code. One of such RNAs is ribosomal RNA (rRNA), which contains several modified bases, particularly around the functionally significant sites. We have focused on understanding the influences of modified base on RNA structure and function by employing helix 69 (H69), which is …
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An Investigation of Bacterial Ribonucleases as an Antibiotic Target
… macrolides specifically target the 30S or 50S subunits to inhibit bacterial growth. During the assembly of the bacterial ribosome, ribosomal RNA of the 30S and 50S ribosomal subunits is processed by bacterial ribonucleases (RNases). RNases are also involved in the degradation and turnover of …
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Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
… larger than ~40 kDa such as proteins, mRNAs, and ribosomal subunits must be aided across by shuttle proteins (karyopherins, or Kaps). Kap-mediated transport involves FG-nups, a family of NPC proteins. While much has been learned about the mechanism of nucleocytoplasmic transport, many details are …
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"fine-tuning" of ribosomal structure and functions by pseudouridylation and rna-protein interactions
<p>ABSTRACT</p> <p>"Fine-tuning" of ribosomal structure and functions by pseudouridylation and RNA-protein interactions</p> <p>by</p> <p>JUN JIANG</p> <p>AUGUST 2012</p> <p>Advisor: Prof. John SantaLucia Jr.</p> <p>Major: Chemistry (Biochemistry)</p> <p>Degree: Doctor of Philosophy</p> <p>Ribosomal …
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Studies on Chloroplast Protein Synthesis In Vivo and In Vitro
… proteins, thylakoid membranes, and chloroplast ribosomal subunits. The use of high specific activity sulfate and improved gel electrophoretic techniques were key features of this study because they permitted minor chloroplast products to be detected for the first time. It was determined that …
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Antibiotics that Inhibit 30S or 50S Ribosomal Subunit Formation: Hygromycin B, Quinupristin-Dalfopristin and XRP 2868.
… that prevent translation by binding to ribosomal subunits have been shown to also inhibit ribosomal subunit assembly (Champney and Tober 2003). The aminoglycoside hygromycin B was examined in <em>Escherichia coli</em> cells for inhibitory effects on translation and ribosomal subunit …
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Elucidating the Roles of Eukaryotic Initiation Factors Involved in DAP5 Mediated Translation
… interplay between messenger RNAs (mRNAs), ribosomal subunits, and a host of initiation factors, ensuring precise start codon selection and the subsequent assembly of the translation machinery. This process has well been known to be mediated by the eukaryotic Initiation Factor (eIF4F), which …
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The Impact of Depleting the Essential Bacterial Enzyme Peptidyl-tRNA Hydrolase on Translation
… Pth can process pep-tRNAs associated with large ribosomal subunits and possibly even translating ribosomes. The discovery of these additional Pth substrates demonstrates that the physiological role of Pth may be more complex than was previously thought and brings longstanding beliefs about the …
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Mechanisms of persistent translation arrest following global brain ischemia and reperfusion
… the focus onto mRNA metabolism and away from ribosomal molecular biology. I have identified new pathways to investigate for understanding why there is selective delayed death in post-ischemic neurons, however my work also gives insight into why resistant neurons survive. The results were …
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Translational Control by the Ribosome-Associated Complex in the Unfolded Protein Response
… and the decoding center on the 60S and 40S ribosomal subunits, respectively. Its unique position on the ribosome implies the coordinating role of de novo protein folding with translation. Deletion of RAC causes growth defects and sensitizes to osmotic, cold, and aminoglycoside stresses in …
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Investigating the Molecular Mechanism of Ribosome Recycling
… a key step during protein synthesis that ensures ribosomal subunits remain available for new rounds of translation, has been largely overlooked. Despite being essential to the survival of the cell, several mechanistic aspects of ribosome recycling remain unclear. Aminoglycosides are a class of …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… greater than 28S were comprised mostly of free ribosomal subunits associated with eRF1. Glucose deprivation and cycloheximide stress treatments revealed that these complexes are likely post-termination complexes rather than pre-initiation or translationally active complexes.</p><p>Additional …
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Dissecting SBDS function in Arabidopsis thaliana
… contributing to its eviction from the 60S ribosomal subunit as part of a ribosome quality control system. Furthermore, I propose that this domain plays a critical role in ribosome biogenesis and plant viability. Using A. thaliana as a model organism, this study investigates the functional …
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An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… as the relative rotation of the large and small ribosomal subunits during protein synthesis. Studying these motions can provide insight into the mechanics of enzyme function that cannot be obtained by measuring its localization or chemical output alone. Rotational tracking can be done in the …
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Proteomics and dynamics of the human nucleolus
… established to be the site where the majority of ribosomal RNAs (5.8S, 18S and 28S) are transcribed, processed and assembled with ribosomal proteins to form ribosomal subunits. The sole role of ribosome biogenesis, however, cannot explain the specific nucleolar localisations of tumour suppressors, …
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Tracking translation factors in fission yeast nucleus
… the ribosome, yet it has been reported that many ribosomal proteins (RPs) and other translation factors are found at transcription sites of Drosophila melanogaster (D. melanogaster) polytene chromosomes. Whilst these findings might indicate the presence of ribosomal subunits at transcription …
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Mining common genetic Variants impacting on Allele-Specific Translation and cancer risk
… to ribonucleoprotein particles (RNPs) and 40S ribosomal subunits, suggesting functional similarity. Notably, the alternative allele produced higher levels of functional eIF4H protein, suggesting the rs1554710467 as a potential gain-of-function variant. Taken collectively, this thesis …
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