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Showing 1 to 20 of 177 for “"RFLP"”.
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Isozyme and RFLP Studies in the Genus Glycine Willd. Subgenus Glycine
… in the subgenus Glycine utilizing isozyme and RFLP analyses. Three studies were conducted and their results are described below.
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Use of RFLP markers in maize as an aid in selection during inbreeding
… Restriction fragment length polymorphisms (RFLPs) were used as markers to detect favorable quantitative trait alleles in the maize (Zea mays L.) population BS11(FR)C7 which were not in the hybrid 'FRB73 x FRMo17'. Thirty-four RFLP loci representing 16 chromosome arms were used to determine …
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Development and validation of PCR-RFLP assay for identification of Gambierdiscus species in the Greater Caribbean region
… an alternative approach, I developed a PCR-RFLP protocol for efficient, rapid, and cost-effective identification of Gambierdiscus species in the Gulf of Mexico and Caribbean Sea, where CFP cases and Gambierdiscus spp. have been reported. The assay targets the D1-D2 hypervariable regions of …
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic Assignment Tool (PAT). Species belonging to the Class Bacilli were the most abundant …
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Efficient production of doubled haploid plants through antimicrotubule agent treatment of anther-derived maize callus and the RFLP analysis of the maize anther culture response
Antimicrotubule agents, colchicine, amiprophos-methyl (APM), pronamide, oryzalin and trifluralin were used to induce chromosome doubling of maize anther-derived haploid callus before plant regeneration. Without treatment with these agents, all the plants regenerated from the callus were haploid. …
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Karakterisasi genetik udang jari (Metapenaeus elegans De Man, 1907) hasil tangkapan dari Laguna Segara Anakan, Cilacap, Jawa Tengah berdasarkan haplotipe DNA mitokondria dengan menggunakan metode PCR-RFLP
… DNA mitokondria dengan menggunakan metode PCR-RFLP. Jenis penelitian ini adalah penelitian deskriptif. Sampel yang digunakan dalam penelitian ini adalah 7 individu udang jari (Metapenaeus elegans) bagian kaki jalan dan ekor yang diambil dari hasil tangkapan dari Laguna Segara Anakan, Cilacap, …
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Restriction fragment length polymorphism mapping of quantitative and qualitative traits in soybean
… max (L.) Merr.) breeders and geneticists with RFLP markers. The thesis consists of three separate studies;In the first study, quantitative trait loci (QTL) for seed size, protein and oil content and for the content of the fatty acids palmitate, stearate, oleate, linoleate and linolenate were …
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Epidemiología molecular de la tuberculosis en Asturias
… la tuberculosis. Las técnicas moleculares: RFLP-IS6110, MIRU-VNTR y Spoligotyping, son en la actualidad las herramientas más empleadas en los estudios de epidemiología molecular de la tuberculosis. Con el presente estudio nos propusimos valorar la aportación de dos técnicas moleculares de …
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Molekularsystematische Untersuchungen an Vertretern der pflanzenparasitischen Gattung Exobasidium (Basidiomycota)
… phylogenetischer Zusammenhänge mit Hilfe von RFLPs und Sequenzdaten untersucht. Der Schwerpunkt liegt auf Pilzstämmen von europäischen Wirtsarten der Gattungen Vaccinium und Rhododendron, wozu hefeartig wachsende Kulturen der saprotrophen Lebensphase dieser Pilze bearbeitet wurden. Zur …
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Molecular Mapping Of A Soybean Mosaic Virus (SMV) Resistance Gene In Soybean (Glycine Max)
… 136 restriction fragment length polymorphism (RFLP) markers yielded 36 markers showing variation between the two parents. These polymorphic RFLP markers unable to provided any indication of linkage to Rsv3. As an alternative strategy, amplified fragment length polymorphic (AFLP) marker analysis …
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An evaluation of DNA fingerprinting methods for subtyping Salmonella
… restriction fragment length polymorphism (RFLP) analysis, and polymerase chain reaction (PCR) fingerprinting methods were evaluated. Plasmid analysis, including plasmid profiles and plasmid fingerprints have been shown to be useful primarily in short-term investigations of disease outbreak. …
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Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles
… complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase …
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A comparison of community composition analyses for the assessment of responses to wood-ash soil amendment by free-living nematodes
… molecular assessment (morpho-richness using T-RFLP) was responsive to experimental treatments. Trait-based approaches (Maturity Index (MI) and Body Size Spectra (BSS)) were more sensitive to forest harvest and wood-ash amendment treatments. The efficacy of these methods was also qualitatively …
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Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis
… DNA restriction fragment length polymorphism (RFLP) analysis and have the speed, safety, and reduced cost of colorimetric detection. The assessment was made on the basis of: cost, sensitivity, time of operation, ease of operation, and ease of multiple probings. Human DNA was extracted from …
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Diversity of the butyrate-producing microflora of the human gut.
… isolates were grouped by 16S rDNA PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) with the restriction enzyme Alu-I, which proved to give the best ribotype discrimination from a total selection of four restriction enzymes. A total of 18 Alu-I PCR-RFLP …
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Human immunodeficiency virus type-1 distribution in South Africa and the relevance of genetic diversity on vaccine design
… on restriction fragment length polymorphism (RFLP) analysis of 400bp (p17) or 650bp (p17 and 5' p24) long PCR fragments. This strategy was appl i ed to eighty-six samples (Cape Town n=47, Johannesburg n=20, Bloemfontein n=17 and Durban n=2) previously subtyped by either sequence analysis of …
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Molecular Genetics on the Genus Glycine: I. Phylogenetic Study of the Subtribe Glycininae. II. Development of a Universal Soybean Genetic Map
… traits but also on molecular traits such as RFLP, RAPD, AFLP, and SSR data. None of these, however, has been associated with specific chromosomes of soybean. Therefore, this study was conducted with the objective of integrating all available classical genetic i.e. pathological, morphological, …
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High-Resolution Mapping of the Region around the Soybean Virus Resistance Genes, Rsv1 and Rpv1
… and 21 restriction fragment length polymorphism (RFLP) markers. Included in these RFLP markers were seven resistance gene candidate (RGC) and five resistance gene candidate flanking (RGCF) markers. RGC probes encode a protein with homology to previously cloned plant disease resistance genes, and …
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Molecular genetic analysis of host resistance to soybean mosaic virus
… DNA restriction fragment length polymorphisms (RFLPs) and microsatellites (or simple sequence repeats, SSRs) were used as genetic markers to identify the chromosomal location of Rsvl1 in a cross between PI 96983 (resistant) and a susceptible cultivar. Twenty five RFLP and three SSR loci …
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